PMID- 36399925
OWN - NLM
STAT- MEDLINE
DCOM- 20221215
LR  - 20230117
IS  - 1876-7753 (Electronic)
IS  - 1873-5061 (Linking)
VI  - 65
DP  - 2022 Dec
TI  - Stem cell sheet fabrication from human umbilical cord mesenchymal stem cell and 
      Col-T scaffold.
PG  - 102960
LID - S1873-5061(22)00309-9 [pii]
LID - 10.1016/j.scr.2022.102960 [doi]
AB  - Today, stem cell therapy has been shown to be a remarkable progress and an 
      important application in the regeneration of defective tissues and organs. To 
      deliver stem cells to the injured area, several methods have been proposed such 
      as an intravenous infusion, direct damaged tissue injection, or stem cell sheet 
      transplantation. In this study, we aimed to fabricate a stem cell sheet by 
      culturing human umbilical cord mesenchymal stem cells (hUC-MSCs) on a Col-T 
      scaffold to recover the structure and function of damaged tissues. The results 
      showed that cells reach confluent on the scaffold surface 18 h after seeding. 
      These stem cells were able to survive and proliferate on Col-T scaffold. The 
      average tensile strength of the stem cell sheet was 2.65 MPa. The sheet reached 
      the sterile standards when tested for total bacteria, Candida albicans, 
      Pseudomonas aeruginosa, and Staphylococcus aureus according to Circular number 
      06/2011/TT-BYT of Vietnam Ministry of Health. In addition, the stem cell sheet 
      was non-toxic when evaluated for exposure toxicity and fluid toxicity according 
      to iSO-10993. Importantly, 5 days after culturing on the Col-T scaffold, the 
      seeded hUC-MSCs were still possessed all properties of MSC such as 
      spindle-shaped, adhesive, could differentiate into mesoderm-derived cells, showed 
      to be CD90, CD105, CD73 positive and CD45, CD34, CD11b, CD19, HLA-DR negative. In 
      summary, our study was successful in creating a stem cell sheet from hUC-MSCs and 
      Col-T scaffold for subsequent in vivo transplantation in the future.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Le-Buu Pham, Truc
AU  - Le-Buu Pham T
AD  - Biotechnology Center of Ho Chi Minh City 700000, Viet Nam.
FAU - Nguyen, Tram Mai
AU  - Nguyen TM
AD  - University of Copenhagen, Norregade 10, 1165 Kobenhavn, Denmark.
FAU - Phu-Hai Nguyen, Dang
AU  - Phu-Hai Nguyen D
AD  - International University, Vietnam National University, 700000, Ho Chi Minh City, 
      Viet Nam.
FAU - Tran, Huynh Nhu
AU  - Tran HN
AD  - Biotechnology Center of Ho Chi Minh City 700000, Viet Nam.
FAU - Thi-Thanh Nguyen, Tam
AU  - Thi-Thanh Nguyen T
AD  - Biotechnology Center of Ho Chi Minh City 700000, Viet Nam.
FAU - Binh, Nguyen Trong
AU  - Binh NT
AD  - Biotechnology Center of Ho Chi Minh City 700000, Viet Nam.
FAU - Nguyen, Quan Dang
AU  - Nguyen QD
AD  - Biotechnology Center of Ho Chi Minh City 700000, Viet Nam. Electronic address: 
      ndquan.snn@tphcm.gov.vn.
FAU - Bui, Hong-Thuy
AU  - Bui HT
AD  - International University, Vietnam National University, 700000, Ho Chi Minh City, 
      Viet Nam. Electronic address: bhthuy@hcmiu.edu.vn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221107
PL  - England
TA  - Stem Cell Res
JT  - Stem cell research
JID - 101316957
SB  - IM
MH  - Humans
MH  - *Umbilical Cord
MH  - Stem Cells
MH  - *Mesenchymal Stem Cells
OTO - NOTNLM
OT  - Cell sheet
OT  - Col-T scaffold
OT  - Mesenchymal stem cells
OT  - Regenerative medicine
OT  - Umbilical cord
COIS- Declaration of Competing Interest The authors declare the following financial 
      interests/personal relationships which may be considered as potential competing 
      interests: Pham Le Buu Truc reports financial support was provided by 
      Biotechnology Center of Ho Chi Minh City, Vietnam. Bui Hong Thuy reports a 
      relationship with International University that includes: board membership. The 
      remaining authors declare that they have no known competing financial interests 
      or personal relationships that could have appeared to influence the work reported 
      in this paper.
EDAT- 2022/11/19 06:00
MHDA- 2022/12/15 06:00
CRDT- 2022/11/18 18:22
PHST- 2022/05/13 00:00 [received]
PHST- 2022/11/02 00:00 [revised]
PHST- 2022/11/04 00:00 [accepted]
PHST- 2022/11/19 06:00 [pubmed]
PHST- 2022/12/15 06:00 [medline]
PHST- 2022/11/18 18:22 [entrez]
AID - S1873-5061(22)00309-9 [pii]
AID - 10.1016/j.scr.2022.102960 [doi]
PST - ppublish
SO  - Stem Cell Res. 2022 Dec;65:102960. doi: 10.1016/j.scr.2022.102960. Epub 2022 Nov 
      7.