PMID- 36403477
OWN - NLM
STAT- MEDLINE
DCOM- 20230126
LR  - 20230201
IS  - 1448-5990 (Electronic)
IS  - 1031-3613 (Linking)
VI  - 35
IP  - 3
DP  - 2023 Jan
TI  - Persistent organic pollutants affect steroidogenic and apoptotic activities in 
      granulosa cells and reactive oxygen species concentrations in oocytes in the 
      mouse.
PG  - 294-305
LID - 10.1071/RD21326 [doi]
AB  - CONTEXT: The destruction of granulosa cells (GCs), the main functional cell type 
      in the ovary, prevents steroid hormone production, which in turn may damage 
      oocytes, resulting in ovarian failure. The accumulation of a number of persistent 
      organic pollutants (POPs) in the ovarian follicular fluid (FF) has been 
      documented, which raises serious questions regarding their impact on female 
      fertility. AIMS: We aimed to determine whether a mixture of POPs reflecting the 
      profile found in FF influences mouse GCs or oocyte function and viability. 
      METHODS: A mixture of POPs, comprising perfluorooctanoate, perfluorooctane 
      sulfonate, 2,2-dichlorodiphenyldichloroethylene, polychlorinated biphenyl 153, 
      and hexachlorobenzene, was used. In addition to using the exact concentration of 
      POPs previously measured in human FF, we tested two other mixtures, one 
      with10-fold lower and another with 10-fold higher concentrations of each POP. KEY 
      RESULTS: Steroidogenesis was disrupted in GCs by the POP mixture, as demonstrated 
      by lower oestradiol and progesterone secretion and greater lipid droplet 
      accumulation. Furthermore, the POP mixture reduced GC viability and increased 
      apoptosis, assessed using caspase-3 activity. The POP mixture significantly 
      increased the number of oocytes that successfully progressed to the second 
      meiotic metaphase and the oocyte reactive oxygen species (ROS) concentration. 
      CONCLUSIONS: Thus, a mixture of POPs that are typically present in human FF has 
      detrimental effects on ovarian function: it reduces the viability of GCs, and 
      increases the oocyte concentrations of ROS. IMPLICATIONS: These results indicate 
      that chronic exposure to POPs adversely affects female reproductive health.
FAU - Krawczyk, Kinga
AU  - Krawczyk K
AD  - Laboratory of Physiology and Toxicology of Reproduction, Institute of Zoology and 
      Biomedical Research, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, 
      Poland.
FAU - Marynowicz, Weronika
AU  - Marynowicz W
AD  - Laboratory of Physiology and Toxicology of Reproduction, Institute of Zoology and 
      Biomedical Research, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, 
      Poland.
FAU - Pich, Karolina
AU  - Pich K
AD  - Laboratory of Physiology and Toxicology of Reproduction, Institute of Zoology and 
      Biomedical Research, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, 
      Poland.
FAU - Jedruch, Oliwia
AU  - Jedruch O
AD  - Laboratory of Genetics and Evolution, Institute of Zoology and Biomedical 
      Research, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland.
FAU - Kania, Gabriela
AU  - Kania G
AD  - Laboratory of Genetics and Evolution, Institute of Zoology and Biomedical 
      Research, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland.
FAU - Gogola-Mruk, Justyna
AU  - Gogola-Mruk J
AD  - Laboratory of Physiology and Toxicology of Reproduction, Institute of Zoology and 
      Biomedical Research, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, 
      Poland.
FAU - Tworzydlo, Waclaw
AU  - Tworzydlo W
AD  - Department of Developmental Biology and Invertebrate Morphology, Institute of 
      Zoology and Biomedical Research, Jagiellonian University, Gronostajowa 9, 30-387 
      Krakow, Poland.
FAU - Polanski, Zbigniew
AU  - Polanski Z
AD  - Laboratory of Genetics and Evolution, Institute of Zoology and Biomedical 
      Research, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland.
FAU - Ptak, Anna
AU  - Ptak A
AD  - Laboratory of Physiology and Toxicology of Reproduction, Institute of Zoology and 
      Biomedical Research, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, 
      Poland.
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Persistent Organic Pollutants)
RN  - 0 (Environmental Pollutants)
SB  - IM
MH  - Female
MH  - Animals
MH  - Humans
MH  - Mice
MH  - Reactive Oxygen Species/metabolism
MH  - *Persistent Organic Pollutants/metabolism
MH  - Granulosa Cells/metabolism
MH  - Oocytes/metabolism
MH  - *Environmental Pollutants/toxicity
EDAT- 2022/11/21 06:00
MHDA- 2023/01/27 06:00
CRDT- 2022/11/20 18:19
PHST- 2021/11/12 00:00 [received]
PHST- 2022/10/28 00:00 [accepted]
PHST- 2022/11/21 06:00 [pubmed]
PHST- 2023/01/27 06:00 [medline]
PHST- 2022/11/20 18:19 [entrez]
AID - RD21326 [pii]
AID - 10.1071/RD21326 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 2023 Jan;35(3):294-305. doi: 10.1071/RD21326.