PMID- 36403793
OWN - NLM
STAT- MEDLINE
DCOM- 20230310
LR  - 20230316
IS  - 1879-2650 (Electronic)
IS  - 0005-2728 (Linking)
VI  - 1864
IP  - 2
DP  - 2023 Apr 1
TI  - Individual heme a and heme a(3) contributions to the Soret absorption spectrum of 
      the reduced bovine cytochrome c oxidase.
PG  - 148937
LID - S0005-2728(22)00407-8 [pii]
LID - 10.1016/j.bbabio.2022.148937 [doi]
AB  - Bovine cytochrome c oxidase (CcO) contains two hemes, a and a(3), chemically 
      identical but differing in coordination and spin state. The Soret absorption band 
      of reduced aa(3)-type cytochrome c oxidase consists of overlapping bands of the 
      hemes a(2+) and a(3)(2+). It shows a peak at  approximately 444 nm and a distinct shoulder at 
       approximately 425 nm. However, attribution of individual spectral lineshapes to hemes a(2+) 
      and a(3)(2+) in the Soret is controversial. In the present work, we characterized 
      spectral contributions of hemes a(2+) and a(3)(2+) using two approaches. First, 
      we reconstructed bovine CcO heme a(2+) spectrum using a selective Ca(2+)-induced 
      spectral shift of the heme a(2+). Second, we investigated photobleaching of the 
      reduced Thermus thermophilus ba(3)- and bovine aa(3)-oxidases in the Soret 
      induced by femtosecond laser pulses in the Q-band. The resolved spectra show 
      splitting of the electronic B(0x)-, B(0y)-transitions of both reduced hemes. The 
      heme a(2+) spectrum is shifted to the red relative to heme a(3)(2+) spectrum. The 
       approximately 425 nm shoulder is mostly attributed to heme a(3)(2+).
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Diuba, Artem V
AU  - Diuba AV
AD  - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State 
      University, Leninskie Gory 1, Bld.40, Moscow 119992, Russia. Electronic address: 
      dyubon@gmail.com.
FAU - Vygodina, Tatiana V
AU  - Vygodina TV
AD  - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State 
      University, Leninskie Gory 1, Bld.40, Moscow 119992, Russia. Electronic address: 
      vygodina@belozersky.msu.ru.
FAU - Azarkina, Natalia V
AU  - Azarkina NV
AD  - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State 
      University, Leninskie Gory 1, Bld.40, Moscow 119992, Russia. Electronic address: 
      azarkina@yahoo.com.
FAU - Arutyunyan, Alexander M
AU  - Arutyunyan AM
AD  - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State 
      University, Leninskie Gory 1, Bld.40, Moscow 119992, Russia. Electronic address: 
      alarut@genebee.msu.ru.
FAU - Soulimane, Tewfik
AU  - Soulimane T
AD  - Materials and Surface Science Institute, University of Limerick, V94 T9PX, 
      Ireland. Electronic address: Tewfik.Soulimane@ul.ie.
FAU - Vos, Marten H
AU  - Vos MH
AD  - LOB, CNRS, INSERM, Ecole Polytechnique, Institut Polytechnique de Paris, 
      Palaiseau 91120, France. Electronic address: marten.vos@polytechnique.edu.
FAU - Konstantinov, Alexander A
AU  - Konstantinov AA
AD  - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State 
      University, Leninskie Gory 1, Bld.40, Moscow 119992, Russia.
LA  - eng
GR  - 55005615/HHMI/Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221118
PL  - Netherlands
TA  - Biochim Biophys Acta Bioenerg
JT  - Biochimica et biophysica acta. Bioenergetics
JID - 101731706
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - 18535-39-2 (heme a)
RN  - EC 1.- (Oxidoreductases)
RN  - 42VZT0U6YR (Heme)
SB  - IM
MH  - Cattle
MH  - Animals
MH  - *Electron Transport Complex IV/metabolism
MH  - Oxidation-Reduction
MH  - *Oxidoreductases/metabolism
MH  - Heme/metabolism
OTO - NOTNLM
OT  - Absorption spectra
OT  - Cytochrome c oxidase
OT  - Femtosecond pump-probe spectroscopy
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/11/21 06:00
MHDA- 2023/03/11 06:00
CRDT- 2022/11/20 19:33
PHST- 2022/05/30 00:00 [received]
PHST- 2022/10/24 00:00 [revised]
PHST- 2022/11/11 00:00 [accepted]
PHST- 2022/11/21 06:00 [pubmed]
PHST- 2023/03/11 06:00 [medline]
PHST- 2022/11/20 19:33 [entrez]
AID - S0005-2728(22)00407-8 [pii]
AID - 10.1016/j.bbabio.2022.148937 [doi]
PST - ppublish
SO  - Biochim Biophys Acta Bioenerg. 2023 Apr 1;1864(2):148937. doi: 
      10.1016/j.bbabio.2022.148937. Epub 2022 Nov 18.