PMID- 36403919
OWN - NLM
STAT- MEDLINE
DCOM- 20221216
LR  - 20221221
IS  - 1567-7257 (Electronic)
IS  - 1567-1348 (Linking)
VI  - 106
DP  - 2022 Dec
TI  - Tracing the origin of an imported HIV-1 sub-subtype A6 strain first identified in 
      Zhejiang Province, China.
PG  - 105388
LID - S1567-1348(22)00185-X [pii]
LID - 10.1016/j.meegid.2022.105388 [doi]
AB  - Human migration and mobility have been identified as key drivers of HIV 
      dissemination among nations, which increases the problem of genetic diversity. 
      Here, we report the near full-length genome of HIV-1 A6 identified in a female 
      patient in the remote mountain area of Lishui, Zhejiang Province, which is the 
      first time A6 has been reported in China. The near full-length genome was 
      amplified with two large amplicons of 5.5 kb and 3.7 kb, and then target PCR 
      products were sequenced by Sanger sequencing. The A6 strain was confirmed by the 
      Basic Local Alignment Search Tool (BLAST) and a maximum-likelihood (ML) 
      phylogenetic tree. The time to the most recent common ancestor (tMRCA) was 
      inferred to be 2004 (95% HPD interval: 2003-2006). The sequence harbored the L74I 
      mutation, which is a key characteristic genetic marker of A6. Combining the above 
      evidence with epidemiological investigations, this A6 strain was determined to be 
      from Ukraine, which was supported by phylogenetic analysis. This study identified 
      a foreign imported strain, indicating a trend of increasing complication in the 
      HIV-1 epidemic in Zhejiang, China.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Zhang, Jiafeng
AU  - Zhang J
AD  - Department of HIV/AIDS & STD Control and Prevention, Zhejiang Provincial Center 
      for Disease Control and Prevention, Hangzhou, China.
FAU - Fan, Qin
AU  - Fan Q
AD  - Department of HIV/AIDS & STD Control and Prevention, Zhejiang Provincial Center 
      for Disease Control and Prevention, Hangzhou, China.
FAU - Ye, Ling
AU  - Ye L
AD  - Lishui Prefectural Center for Disease Control and Prevention, Lishui, China.
FAU - Liu, Jing
AU  - Liu J
AD  - School of Laboratory Medicine and Life Science, Wenzhou Medical University, 
      Wenzhou, China.
FAU - Chen, Xiaolei
AU  - Chen X
AD  - Lishui Prefectural Center for Disease Control and Prevention, Lishui, China.
FAU - Li, Xingguang
AU  - Li X
AD  - Department of Technology R&D, Ningbo Institute of Life and Health Industry, 
      University of Chinese Academy of Sciences, Ningbo, China; Hwa Mei Hospital, 
      University of Chinese Academy of Sciences, Ningbo, China.
FAU - Chai, Chengliang
AU  - Chai C
AD  - Department of HIV/AIDS & STD Control and Prevention, Zhejiang Provincial Center 
      for Disease Control and Prevention, Hangzhou, China. Electronic address: 
      chlchai@cdc.zj.cn.
LA  - eng
PT  - Journal Article
DEP - 20221117
PL  - Netherlands
TA  - Infect Genet Evol
JT  - Infection, genetics and evolution : journal of molecular epidemiology and 
      evolutionary genetics in infectious diseases
JID - 101084138
SB  - IM
MH  - Humans
MH  - *HIV-1/genetics
MH  - Phylogeny
MH  - China/epidemiology
MH  - Ukraine
MH  - *HIV Infections/epidemiology
OTO - NOTNLM
OT  - A6
OT  - HIV-1
OT  - Near full-length genome
COIS- Declaration of Competing Interest The authors declare that there are no conflicts 
      of interest.
EDAT- 2022/11/21 06:00
MHDA- 2022/12/15 06:00
CRDT- 2022/11/20 19:35
PHST- 2022/08/26 00:00 [received]
PHST- 2022/11/14 00:00 [revised]
PHST- 2022/11/16 00:00 [accepted]
PHST- 2022/11/21 06:00 [pubmed]
PHST- 2022/12/15 06:00 [medline]
PHST- 2022/11/20 19:35 [entrez]
AID - S1567-1348(22)00185-X [pii]
AID - 10.1016/j.meegid.2022.105388 [doi]
PST - ppublish
SO  - Infect Genet Evol. 2022 Dec;106:105388. doi: 10.1016/j.meegid.2022.105388. Epub 
      2022 Nov 17.