PMID- 36407109
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221122
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 10
DP  - 2022
TI  - Liver mitochondria-associated endoplasmic reticulum membrane proteomics for 
      studying the effects of ZiBuPiYin recipe on Zucker diabetic fatty rats after 
      chronic psychological stress.
PG  - 995732
LID - 10.3389/fcell.2022.995732 [doi]
LID - 995732
AB  - Type 2 diabetes mellitus (T2DM) is a complex metabolic disease with multiple 
      etiologies, involving both genetic and environmental factors. With changes 
      associated with modern life, increasing attention has been paid to chronic 
      psychological stressors such as work stress. Chronic psychological stress can 
      induce or aggravate diabetes mellitus, and conversely, with the deterioration of 
      T2DM, patients often experience different degrees of depression, anxiety, and 
      other negative emotions. In order to clarify the role of ZiBuPiYin recipe (ZBPYR) 
      in regulating the liver mitochondria-associated endoplasmic reticulum membrane 
      proteome to improve T2DM with chronic psychological stress, differentially 
      expressed proteins (DEPs) were identified among Zucker lean littermates (control 
      group), chronic psychological stress T2DM rats (model group), and ZBPYR 
      administration rats (ZBPYR group) through iTRAQ with LC-MS/MS. Using Mfuzz soft 
      clustering analysis, DEPs were divided into six different clusters. Clusters 1-6 
      contained 5, 68, 44, 57, 28, and 32 DEPs, respectively. Given that ZBPYR can 
      alleviate T2DM symptoms and affect exploratory behavior during T2DM with chronic 
      psychological stress, we focused on the clusters with opposite expression trends 
      between model:control and ZBPYR:model groups. We screened out the DEPs in 
      clusters 1, 3, and 4, which may be good candidates for the prevention and 
      treatment of T2DM with chronic psychological stress, and further conducted 
      bioinformatics analyses. DEPs were mainly involved in the insulin signaling 
      pathway, oxidative phosphorylation, tricarboxylic acid cycle, amino acid 
      metabolism, lysosome-related processes, and lipid metabolism. This may indicate 
      the pathogenic basis of T2DM with chronic psychological stress and the potential 
      therapeutic mechanism of ZBPYR. In addition, two key proteins, 
      lysosome-associated protein (Lamp2) and tricarboxylic acid cycle-related protein 
      (Suclg1), may represent novel biomarkers for T2DM with chronic psychological 
      stress and drug targets of ZBPYR. Western blot analyses also showed similar 
      expression patterns of these two proteins in liver MAMs of the model and ZBPYR 
      groups.
CI  - Copyright (c) 2022 Xu, Zhou, Zhan, Bi and Lu.
FAU - Xu, Huiying
AU  - Xu H
AD  - Modern Research Laboratory of Spleen Visceral Manifestations Theory, School of 
      Traditional Chinese Medicine, School of Integrated Chinese and Western Medicine, 
      Nanjing University of Chinese Medicine, Nanjing, China.
FAU - Zhou, Wen
AU  - Zhou W
AD  - Modern Research Laboratory of Spleen Visceral Manifestations Theory, School of 
      Traditional Chinese Medicine, School of Integrated Chinese and Western Medicine, 
      Nanjing University of Chinese Medicine, Nanjing, China.
FAU - Zhan, Libin
AU  - Zhan L
AD  - Center for Innovative Engineering Technology in Traditional Chinese Medicine, 
      Liaoning University of Traditional Chinese Medicine, Shenyang, China.
AD  - Key Laboratory of Ministry of Education for TCM Viscera-State Theory and 
      Applications, Liaoning University of Traditional Chinese Medicine, Shenyang, 
      China.
FAU - Bi, Tingting
AU  - Bi T
AD  - Modern Research Laboratory of Spleen Visceral Manifestations Theory, School of 
      Traditional Chinese Medicine, School of Integrated Chinese and Western Medicine, 
      Nanjing University of Chinese Medicine, Nanjing, China.
FAU - Lu, Xiaoguang
AU  - Lu X
AD  - Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20221103
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC9669571
OTO - NOTNLM
OT  - ZiBuPiYin recipe
OT  - chronic psychological stress
OT  - mitochondria-associated ER membrane
OT  - proteomics
OT  - type 2 diabetes mellitus
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/11/22 06:00
MHDA- 2022/11/22 06:01
PMCR- 2022/01/01
CRDT- 2022/11/21 04:28
PHST- 2022/07/16 00:00 [received]
PHST- 2022/10/18 00:00 [accepted]
PHST- 2022/11/21 04:28 [entrez]
PHST- 2022/11/22 06:00 [pubmed]
PHST- 2022/11/22 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 995732 [pii]
AID - 10.3389/fcell.2022.995732 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2022 Nov 3;10:995732. doi: 10.3389/fcell.2022.995732. 
      eCollection 2022.