PMID- 36408965
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231103
IS  - 2049-3614 (Print)
IS  - 2049-3614 (Electronic)
IS  - 2049-3614 (Linking)
VI  - 12
IP  - 1
DP  - 2023 Jan 1
TI  - Long-term effects of prenatal undernutrition on female rat hypothalamic KNDy 
      neurons.
LID - e220307 [pii]
LID - 10.1530/EC-22-0307 [doi]
AB  - The nutritional environment during development periods induces metabolic 
      programming, leading to metabolic disorders and detrimental influences on human 
      reproductive health. This study aimed to determine the long-term adverse effect 
      of intrauterine malnutrition on the reproductive center kisspeptin-neurokinin 
      B-dynorphin A (KNDy) neurons in the hypothalamic arcuate nucleus (ARC) of female 
      offspring. Twelve pregnant rats were divided into ad-lib-fed (control, n = 6) and 
      50% undernutrition (UN, n = 6) groups. The UN group was restricted to 50% daily 
      food intake of the control dams from gestation day 9 until term delivery. 
      Differences between the two groups in terms of various maternal parameters, 
      including body weight (BW), pregnancy duration, and litter size, as well as birth 
      weight, puberty onset, estrous cyclicity, pulsatile luteinizing hormone (LH) 
      secretion, and hypothalamic gene expression of offspring, were determined. Female 
      offspring of UN dams exhibited low BW from birth to 3 weeks, whereas UN offspring 
      showed signs of precocious puberty; hypothalamic Tac3 (a neurokinin B gene) 
      expression was increased in prepubertal UN offspring, and the BW at the virginal 
      opening was lower in UN offspring than that in the control group. Interestingly, 
      the UN offspring showed significant decreases in the number of KNDy 
      gene-expressing cells after 29 weeks of age, but the number of ARC 
      kisspeptin-immunoreactive cells, pulsatile LH secretions, and estrous cyclicity 
      were comparable between the groups. In conclusion, intrauterine undernutrition 
      induced various changes in KNDy gene expression depending on the life stage. 
      Thus, intrauterine undernutrition affected hypothalamic developmental programming 
      in female rats.
FAU - Minabe, Shiori
AU  - Minabe S
AUID- ORCID: 0000-0001-7902-925X
AD  - Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon 
      Medical School, Tokyo, Japan.
AD  - Division of Biomedical Information Analysis, Iwate Tohoku Medical Megabank 
      Organization, Disaster Reconstruction Center, Iwate Medical University, Yahaba, 
      Japan.
FAU - Iwata, Kinuyo
AU  - Iwata K
AD  - Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon 
      Medical School, Tokyo, Japan.
FAU - Watanabe, Youki
AU  - Watanabe Y
AD  - Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon 
      Medical School, Tokyo, Japan.
FAU - Ishii, Hirotaka
AU  - Ishii H
AD  - Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon 
      Medical School, Tokyo, Japan.
FAU - Ozawa, Hitoshi
AU  - Ozawa H
AD  - Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon 
      Medical School, Tokyo, Japan.
AD  - Faculty of Health Science, Bukkyo University, Kyoto, Japan.
LA  - eng
PT  - Journal Article
DEP - 20221215
PL  - England
TA  - Endocr Connect
JT  - Endocrine connections
JID - 101598413
PMC - PMC9782422
OTO - NOTNLM
OT  - fetal programing
OT  - kisspeptin
OT  - low birth weight
OT  - prenatal undernutrition
COIS- The authors declare no conflict of interest.
EDAT- 2022/11/22 06:00
MHDA- 2022/11/22 06:01
PMCR- 2022/12/15
CRDT- 2022/11/21 07:52
PHST- 2022/11/07 00:00 [received]
PHST- 2022/11/21 00:00 [accepted]
PHST- 2022/11/22 06:00 [pubmed]
PHST- 2022/11/22 06:01 [medline]
PHST- 2022/11/21 07:52 [entrez]
PHST- 2022/12/15 00:00 [pmc-release]
AID - e220307 [pii]
AID - EC-22-0307 [pii]
AID - 10.1530/EC-22-0307 [doi]
PST - epublish
SO  - Endocr Connect. 2022 Dec 15;12(1):e220307. doi: 10.1530/EC-22-0307. Print 2023 
      Jan 1.