PMID- 36410115
OWN - NLM
STAT- MEDLINE
DCOM- 20221220
LR  - 20221222
IS  - 2352-3964 (Electronic)
IS  - 2352-3964 (Linking)
VI  - 86
DP  - 2022 Dec
TI  - Comprehensive morphometric assessment of deltoid muscle development in children: 
      A cross-sectional study.
PG  - 104367
LID - S2352-3964(22)00549-7 [pii]
LID - 10.1016/j.ebiom.2022.104367 [doi]
LID - 104367
AB  - BACKGROUND: Normative values for different morphometric parameters of muscle 
      fibres during paediatric development, i.e. from 0 to 18 years, are currently 
      unavailable. They would be of major importance to accurately evaluate 
      pathological changes and could be used as reference biomarkers for evaluating 
      treatment response in clinical trials, or physiological adjustments in sports or 
      ageing. METHODS: Data were derived from 482 images with a total of 33 094 fibres 
      from 10 mum cross-sections of snap-frozen muscle from 83 deltoid muscle biopsies 
      from patients, 0-18 years, without neuromuscular pathology stained with ATPase 
      9.4. Data was acquired and analysed with patented image analysis algorithms from 
      "CARPACCIO.cloud". Several parameters were extracted or calculated, including 
      cross-sectional area (CSA), fibre type, circularity, as well as the Minimum 
      diameter of Feret (MinFeret). FINDINGS: This study illustrates changes in 
      quantitative parameters for muscle morphology over the course of paediatric 
      development and the pivotal changes occurring around puberty. Only fibre size 
      parameters (MinFeret, CSA) are dependent on gender, and only after puberty. All 
      other parameters vary in a similar manner for females and males. The proportion 
      of type 1 fibres is essentially constant from birth to age 10, decreasing to  approximately 40% 
      by age 18. Circularity decreases with age, to plateau after age 10 for both fibre 
      types. INTERPRETATION: Normative values and reference charts for muscle fibre 
      types in this age range have been generated to allow comparison of data from 
      patients in pathology laboratories working on neuromuscular diseases. FUNDING: 
      BPI FRANCE, PULSALYS, Association de l'Institut de Myologie, French National 
      Research Agency (ANR), LABEX CORTEX of Universite de Lyon.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Evangelista, Teresinha
AU  - Evangelista T
AD  - AP-HP, H. Pitie-Salpetriere, Institut de Myologie, Unite de Morphologie 
      Neuromusculaire, France; AP-HP, H. Pitie-Salpetriere, Centre de reference des 
      maladies neuromusculaires Nord/Est/Ile de France, France; Sorbonne Universite, 
      INSERM, Institut de Myologie, Centre de Recherche en Myologie, France. Electronic 
      address: t.evangelista@institut-myologie.org.
FAU - Kandji, Malick
AU  - Kandji M
AD  - CARPACCIO.cloud, Lyon, France.
FAU - Lacene, Emmanuelle
AU  - Lacene E
AD  - AP-HP, H. Pitie-Salpetriere, Institut de Myologie, Unite de Morphologie 
      Neuromusculaire, France.
FAU - Chanut, Anais
AU  - Chanut A
AD  - AP-HP, H. Pitie-Salpetriere, Institut de Myologie, Unite de Morphologie 
      Neuromusculaire, France.
FAU - Bui, Mai Thao
AU  - Bui MT
AD  - AP-HP, H. Pitie-Salpetriere, Institut de Myologie, Unite de Morphologie 
      Neuromusculaire, France.
FAU - Marty, Rudy
AU  - Marty R
AD  - CARPACCIO.cloud, Lyon, France.
FAU - Buffat, Laurent
AU  - Buffat L
AD  - AltraBio, Lyon, France.
FAU - Knoblauch, Kenneth
AU  - Knoblauch K
AD  - Univ Lyon, Universite Claude Bernard Lyon 1, Inserm, Stem-cell & Brain Research 
      Institute, Bron, France; National Centre for Optics, Vision and Eye Care, Faculty 
      of Health and Social Sciences, University of South-Eastern Norway, Kongsberg, 
      Norway.
FAU - Rudkin, Brian B
AU  - Rudkin BB
AD  - CARPACCIO.cloud, Lyon, France; Univ Lyon, Universite Claude Bernard Lyon 1, 
      Inserm, Stem-cell & Brain Research Institute, Bron, France; Shanghai Jiao Tong 
      University Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai, 
      PR China. Electronic address: brian.rudkin@inserm.fr.
FAU - Romero, Norma Beatriz
AU  - Romero NB
AD  - AP-HP, H. Pitie-Salpetriere, Institut de Myologie, Unite de Morphologie 
      Neuromusculaire, France; AP-HP, H. Pitie-Salpetriere, Centre de reference des 
      maladies neuromusculaires Nord/Est/Ile de France, France; Sorbonne Universite, 
      INSERM, Institut de Myologie, Centre de Recherche en Myologie, France.
LA  - eng
PT  - Journal Article
DEP - 20221118
PL  - Netherlands
TA  - EBioMedicine
JT  - EBioMedicine
JID - 101647039
SB  - IM
EIN - EBioMedicine. 2022 Dec 14;87:104400. PMID: 36525724
MH  - Male
MH  - Female
MH  - Humans
MH  - Child
MH  - Adolescent
MH  - Cross-Sectional Studies
MH  - *Muscle Development
MH  - *Muscle Fibers, Skeletal
MH  - Biopsy
MH  - Aging
MH  - Muscle, Skeletal
PMC - PMC9677083
OTO - NOTNLM
OT  - Automated analysis
OT  - Human
OT  - Morphometry
OT  - Normal skeletal muscle
OT  - Paediatric age
COIS- Declaration of interests The authors have no conflicts of interests regarding 
      this manuscript. BBR is an inventor on the patent describing the algorithms used 
      in this study.
EDAT- 2022/11/22 06:00
MHDA- 2022/12/21 06:00
PMCR- 2022/11/18
CRDT- 2022/11/21 18:18
PHST- 2022/04/27 00:00 [received]
PHST- 2022/10/31 00:00 [revised]
PHST- 2022/10/31 00:00 [accepted]
PHST- 2022/11/22 06:00 [pubmed]
PHST- 2022/12/21 06:00 [medline]
PHST- 2022/11/21 18:18 [entrez]
PHST- 2022/11/18 00:00 [pmc-release]
AID - S2352-3964(22)00549-7 [pii]
AID - 104367 [pii]
AID - 10.1016/j.ebiom.2022.104367 [doi]
PST - ppublish
SO  - EBioMedicine. 2022 Dec;86:104367. doi: 10.1016/j.ebiom.2022.104367. Epub 2022 Nov 
      18.