PMID- 36415143
OWN - NLM
STAT- MEDLINE
DCOM- 20230307
LR  - 20230307
IS  - 1099-1573 (Electronic)
IS  - 0951-418X (Linking)
VI  - 37
IP  - 3
DP  - 2023 Mar
TI  - Pectolinarigenin shows lipid-lowering effects by inhibiting fatty acid 
      biosynthesis in vitro and in vivo.
PG  - 913-925
LID - 10.1002/ptr.7679 [doi]
AB  - Pectolinarigenin is the main flavonoid compound and presents in Linaria vulgaris 
      and Cirsium chanroenicum. In this study, RNA sequencing (RNA-seq) was applied to 
      dissect the effect of pectolinarigenin on the transcriptome changes in the high 
      lipid Huh-7 cells induced by oleic acid. RNA-seq results revealed that 15 
      pathways enriched by downregulated genes are associated with cell metabolism 
      including cholesterol metabolism, glycerophospholipid metabolism, steroid 
      biosynthesis, steroid hormone biosynthesis, fatty acid biosynthesis, etc. 
      Moreover, 13 key genes related to lipid metabolism were selected. Among them, 
      PPARG coactivator 1 beta (PPARGC1B) and carnitine palmitoyltransferase 1A (CPT1A) 
      were found to be upregulated, solute carrier family 27 member 1(SLC27A1), 
      acetyl-CoA carboxylase alpha (ACACA), fatty-acid synthase (FASN), 
      3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR), etc. were found to be 
      downregulated. Glycolysis/gluconeogenesis, steroid hormone biosynthesis, and 
      fatty acid biosynthesis were all significantly downregulated, according to gene 
      set variation analysis and gene set enrichment analysis. Besides, protein levels 
      of FASN, ACACA, and SLC27A1 were all decreased, whereas PPARgamma and CPT1A were 
      increased. Docking models showed that PPARgamma may be a target for pectolinarigenin. 
      Furthermore, pectolinarigenin reduced serum TG and hepatic TG, and improved 
      insulin sensitivity in vivo. Our findings suggest that pectolinarigenin may 
      target PPARgamma and prevent fatty acid biosynthesis.
CI  - (c) 2022 John Wiley & Sons Ltd.
FAU - Lan, Zhou
AU  - Lan Z
AUID- ORCID: 0000-0001-7210-4181
AD  - Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, Jiangxi Key 
      Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal 
      University, Nanchang, China.
FAU - Zhang, Kun
AU  - Zhang K
AD  - Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, Jiangxi Key 
      Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal 
      University, Nanchang, China.
FAU - He, Jianhui
AU  - He J
AD  - Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, Jiangxi Key 
      Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal 
      University, Nanchang, China.
FAU - Kang, Qiong
AU  - Kang Q
AD  - Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, Jiangxi Key 
      Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal 
      University, Nanchang, China.
FAU - Meng, Wei
AU  - Meng W
AD  - Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, Jiangxi Key 
      Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal 
      University, Nanchang, China.
FAU - Wang, Songhua
AU  - Wang S
AUID- ORCID: 0000-0003-3605-4484
AD  - Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, Jiangxi Key 
      Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal 
      University, Nanchang, China.
LA  - eng
GR  - 31860605/National Natural Science Foundation of China/
GR  - 81860692/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20221122
PL  - England
TA  - Phytother Res
JT  - Phytotherapy research : PTR
JID - 8904486
RN  - 0 (pectolinarigenin)
RN  - 0 (PPAR gamma)
RN  - EC 2.3.1.85 (Fatty Acid Synthases)
RN  - 0 (Fatty Acids)
RN  - 0 (Lipids)
RN  - 0 (Steroids)
RN  - 0 (Hormones)
SB  - IM
MH  - *PPAR gamma/metabolism
MH  - *Liver
MH  - Lipid Metabolism
MH  - Fatty Acid Synthases/metabolism
MH  - Fatty Acids/pharmacology
MH  - Lipids
MH  - Steroids
MH  - Hormones/metabolism/pharmacology
OTO - NOTNLM
OT  - RNA-seq
OT  - flavonoid
OT  - high fat diet
OT  - lipid
OT  - pectolinarigenin
EDAT- 2022/11/24 06:00
MHDA- 2023/03/08 06:00
CRDT- 2022/11/23 01:43
PHST- 2022/10/21 00:00 [revised]
PHST- 2022/08/05 00:00 [received]
PHST- 2022/11/06 00:00 [accepted]
PHST- 2022/11/24 06:00 [pubmed]
PHST- 2023/03/08 06:00 [medline]
PHST- 2022/11/23 01:43 [entrez]
AID - 10.1002/ptr.7679 [doi]
PST - ppublish
SO  - Phytother Res. 2023 Mar;37(3):913-925. doi: 10.1002/ptr.7679. Epub 2022 Nov 22.