PMID- 36415512
OWN - NLM
STAT- MEDLINE
DCOM- 20221125
LR  - 20221125
IS  - 1844-3117 (Electronic)
IS  - 1844-122X (Print)
IS  - 1844-122X (Linking)
VI  - 15
IP  - 9
DP  - 2022 Sep
TI  - Analysis of TERT Rs2736100 genotype distribution in laryngeal squamous cell 
      carcinoma patients.
PG  - 1191-1197
LID - 10.25122/jml-2022-0114 [doi]
AB  - The applicability of the telomerase reverse transcriptase (TERT) gene Rs2736100 
      polymorphism in cancer research has been well documented for various malignancies 
      except for head and neck cancers, where data is sparse. This study aimed to 
      analyze this polymorphism with the pathological characterizers of laryngeal 
      squamous cell carcinoma (LSCC) patients. Genetic testing was performed using the 
      Real-time PCR technique on 56 paired samples of biological material (blood and 
      tissue). Data were analyzed using Epi info 7 software. The subjects were 
      predominantly male (95% vs. 5%), with a median age of 62 years, and smokers 
      (89%). The primary tumor origin site was the glottic region (34%), and the 
      advanced clinical stages III-IV were more common (46% vs. 18%). Results show high 
      frequencies for the mutated variants of Rs2736100 (CC 36%>AC 34%>AA 30%), while 
      distribution according to tumor classification criteria leaned towards moderately 
      differentiated carcinoma specimens in T3-T4 stages for the AC/CC variants 
      (P-value without statistical significance) but positively favored the 
      relationship between the AA variant and lack of lymph node metastasis (P=0.0106). 
      The genotypes tend to associate themselves with a better histological 
      presentation regarding the pattern of tumor invasion and, thus, better prognostic 
      values for LSCC. Results suggest that the wild-type genotype of TERT Rs2736100 
      may be a protective factor for lymph node metastasis and histological pattern of 
      tumor invasion in LSCC. Results regarding the synergistic relationship between 
      cancer and smoking corroborate literature data for moderate to severe smokers 
      regardless of the genetic variant.
CI  - (c)2022 JOURNAL of MEDICINE and LIFE.
FAU - Cornean, Corina Iulia
AU  - Cornean CI
AD  - Department of Otolaryngology-Head and Neck Surgery, Iuliu Hatieganu University of 
      Medicine and Pharmacy, Cluj-Napoca, Romania.
FAU - Maniu, Alma Aurelia
AU  - Maniu AA
AD  - Department of Otolaryngology-Head and Neck Surgery, Iuliu Hatieganu University of 
      Medicine and Pharmacy, Cluj-Napoca, Romania.
LA  - eng
PT  - Journal Article
PL  - Romania
TA  - J Med Life
JT  - Journal of medicine and life
JID - 101477617
RN  - EC 2.7.7.49 (Telomerase)
RN  - EC 2.7.7.49 (TERT protein, human)
SB  - IM
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Genotype
MH  - *Laryngeal Neoplasms/genetics/pathology
MH  - Lymphatic Metastasis
MH  - *Squamous Cell Carcinoma of Head and Neck/genetics/pathology
MH  - *Telomerase/genetics
PMC - PMC9635231
OTO - NOTNLM
OT  - ETDA - ethylenediaminetetraacetic-acid
OT  - G1 - well-differentiated squamous cell carcinoma
OT  - G2 - moderately differentiated squamous cell carcinoma
OT  - G3 - poorly differentiated squamous cell carcinoma
OT  - HNC - head and neck cancer
OT  - LSCC - laryngeal squamous cell carcinoma
OT  - M - distant metastasis
OT  - N - regional node metastasis
OT  - PCR analysis
OT  - Real-time-PCR - Real-time polymerase chain reaction
OT  - SCC - squamous cell carcinoma
OT  - TERC - telomere ARN component
OT  - TERT Rs2736100
OT  - TERT - telomerase reverse transcriptase
OT  - TNM - tumor classification criteria-T (the primary tumor)
OT  - UADT - upper aerodigestive tract
OT  - laryngeal squamous cell carcinoma
COIS- The authors declare no conflicts of interest.
EDAT- 2022/11/24 06:00
MHDA- 2022/11/25 06:00
PMCR- 2022/09/01
CRDT- 2022/11/23 02:08
PHST- 2022/04/13 00:00 [received]
PHST- 2022/08/09 00:00 [accepted]
PHST- 2022/11/23 02:08 [entrez]
PHST- 2022/11/24 06:00 [pubmed]
PHST- 2022/11/25 06:00 [medline]
PHST- 2022/09/01 00:00 [pmc-release]
AID - JMedLife-15-1191 [pii]
AID - 10.25122/jml-2022-0114 [doi]
PST - ppublish
SO  - J Med Life. 2022 Sep;15(9):1191-1197. doi: 10.25122/jml-2022-0114.