PMID- 36417483
OWN - NLM
STAT- MEDLINE
DCOM- 20221128
LR  - 20230106
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 17
IP  - 11
DP  - 2022
TI  - The GGDEF-EAL protein CdgB from Azospirillum baldaniorum Sp245, is a dual 
      function enzyme with potential polar localization.
PG  - e0278036
LID - 10.1371/journal.pone.0278036 [doi]
LID - e0278036
AB  - Azospirillum baldaniorum Sp245, a plant growth-promoting rhizobacterium, can form 
      biofilms through a process controlled by the second messenger cyclic diguanylate 
      monophosphate (c-di-GMP). A. baldaniorum has a variety of proteins potentially 
      involved in controlling the turnover of c-di-GMP many of which are coupled to 
      sensory domains that could be involved in establishing a mutualistic relationship 
      with the host. Here, we present in silico analysis and experimental 
      characterization of the function of CdgB (AZOBR_p410089), a predicted 
      MHYT-PAS-GGDEF-EAL multidomain protein from A. baldaniorum Sp245. When 
      overproduced, CdgB behaves predominantly as a c-di-GMP phosphodiesterase (PDE) in 
      A. baldaniorum Sp245. It inhibits biofilm formation and extracellular polymeric 
      substances production and promotes swimming motility. However, a CdgB variant 
      with a degenerate PDE domain behaves as diguanylate cyclase (DGC). This strongly 
      suggest that CdgB is capable of dual activity. Variants with alterations in the 
      DGC domain and the MHYT domain negatively affects extracellular polymeric 
      substances production and induction of swimming motility. Surprisingly, we 
      observed that overproduction of CdgB results in increased c-di-GMP accumulation 
      in the heterologous host Escherichia coli, suggesting under certain conditions, 
      the WT CdgB variant can behave predominantly as a DGC. Furthermore, we also 
      demonstrated that CdgB is anchored to the cell membrane and localizes potentially 
      to the cell poles. This localization is dependent on the presence of the MHYT 
      domain. In summary, our results suggest that CdgB can provide versatility to 
      signaling modules that control motile and sessile lifestyles in response to key 
      environmental signals in A. baldaniorum.
CI  - Copyright: (c) 2022 Viruega-Gongora et al. This is an open access article 
      distributed under the terms of the Creative Commons Attribution License, which 
      permits unrestricted use, distribution, and reproduction in any medium, provided 
      the original author and source are credited.
FAU - Viruega-Gongora, Victor I
AU  - Viruega-Gongora VI
AD  - Laboratorio de la Interaccion bacteria-planta, Centro de Investigaciones en 
      Ciencias Microbiologicas, Benemerita Universidad Autonoma de Puebla, Puebla Pue, 
      Mexico.
FAU - Acatitla-Jacome, Iris S
AU  - Acatitla-Jacome IS
AD  - Laboratorio de la Interaccion bacteria-planta, Centro de Investigaciones en 
      Ciencias Microbiologicas, Benemerita Universidad Autonoma de Puebla, Puebla Pue, 
      Mexico.
FAU - Zamorano-Sanchez, David
AU  - Zamorano-Sanchez D
AUID- ORCID: 0000-0002-5580-7499
AD  - Programa de Biologia de Sistemas y Biologia Sintetica, Centro de Ciencias 
      Genomicas, Universidad Nacional Autonoma de Mexico, Cuernavaca, Mexico.
FAU - Reyes-Carmona, Sandra R
AU  - Reyes-Carmona SR
AD  - Laboratorio de la Interaccion bacteria-planta, Centro de Investigaciones en 
      Ciencias Microbiologicas, Benemerita Universidad Autonoma de Puebla, Puebla Pue, 
      Mexico.
FAU - Xiqui-Vazquez, Maria L
AU  - Xiqui-Vazquez ML
AD  - Laboratorio de la Interaccion bacteria-planta, Centro de Investigaciones en 
      Ciencias Microbiologicas, Benemerita Universidad Autonoma de Puebla, Puebla Pue, 
      Mexico.
FAU - Baca, Beatriz Eugenia
AU  - Baca BE
AD  - Laboratorio de la Interaccion bacteria-planta, Centro de Investigaciones en 
      Ciencias Microbiologicas, Benemerita Universidad Autonoma de Puebla, Puebla Pue, 
      Mexico.
FAU - Ramirez-Mata, Alberto
AU  - Ramirez-Mata A
AUID- ORCID: 0000-0002-2119-2254
AD  - Laboratorio de la Interaccion bacteria-planta, Centro de Investigaciones en 
      Ciencias Microbiologicas, Benemerita Universidad Autonoma de Puebla, Puebla Pue, 
      Mexico.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221123
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Bacterial Proteins)
RN  - H2D2X058MU (Cyclic GMP)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
SB  - IM
MH  - *Azospirillum
MH  - *Bacterial Proteins/genetics/metabolism
MH  - Cyclic GMP/metabolism
MH  - Gene Expression Regulation, Bacterial
MH  - Phosphoric Diester Hydrolases/metabolism
PMC - PMC9683572
COIS- The authors have declared that no competing interests exist.
EDAT- 2022/11/24 06:00
MHDA- 2022/11/26 06:00
PMCR- 2022/11/23
CRDT- 2022/11/23 13:54
PHST- 2022/08/02 00:00 [received]
PHST- 2022/11/08 00:00 [accepted]
PHST- 2022/11/23 13:54 [entrez]
PHST- 2022/11/24 06:00 [pubmed]
PHST- 2022/11/26 06:00 [medline]
PHST- 2022/11/23 00:00 [pmc-release]
AID - PONE-D-22-21548 [pii]
AID - 10.1371/journal.pone.0278036 [doi]
PST - epublish
SO  - PLoS One. 2022 Nov 23;17(11):e0278036. doi: 10.1371/journal.pone.0278036. 
      eCollection 2022.