PMID- 36419253
OWN - NLM
STAT- MEDLINE
DCOM- 20230131
LR  - 20230202
IS  - 1793-6853 (Electronic)
IS  - 0192-415X (Linking)
VI  - 51
IP  - 1
DP  - 2023
TI  - Andrographolide Inhibits Lipotoxicity-Induced Activation of the NLRP3 
      Inflammasome in Bone Marrow-Derived Macrophages.
PG  - 129-147
LID - 10.1142/S0192415X23500088 [doi]
AB  - Andrographolide is the major bioactive component of the herb Andrographis 
      paniculata and is a potent anti-inflammatory agent. Obesity leads to an excess of 
      free fatty acids, particularly palmitic acid (PA), in the circulation. Obesity 
      also causes the deposition of ectopic fat in nonadipose tissues, which leads to 
      lipotoxicity, a condition closely associated with inflammation. Here, we 
      investigated whether andrographolide could inhibit PA-induced inflammation by 
      activating autophagy, activating the antioxidant defense system, and blocking the 
      activation of the NLRP3 inflammasome. Bone marrow-derived macrophages (BMDMs) 
      were primed with lipopolysaccharide (LPS) and then activated with PA. LPS/PA 
      treatment increased both the mRNA expression of NLRP3 and IL-1[Formula: see text] 
      and the release of IL-1[Formula: see text] in BMDMs. Andrographolide inhibited 
      the LPS/PA-induced protein expression of caspase-1 and the release of 
      IL-1[Formula: see text]. Furthermore, andrographolide attenuated LPS/PA-induced 
      mtROS generation by first promoting autophagic flux and catalase activity, and 
      ultimately inhibiting activation of the NLRP3 inflammasome. Our results suggest 
      that the mechanisms by which andrographolide downregulates LPS/PA-induced 
      IL-1[Formula: see text] release in BMDMs involve promoting autophagic flux and 
      catalase activity. Andrographolide may thus be a candidate to prevent obesity- 
      and lipotoxicity-driven chronic inflammatory disease.
FAU - Yen, Chih-Ching
AU  - Yen CC
AD  - Department of Respiratory Therapy, China Medical University, Taichung, Taiwan.
AD  - Department of Internal Medicine, China Medical University Hospital, Taichung, 
      Taiwan.
FAU - Lii, Chong-Kuei
AU  - Lii CK
AD  - Department of Nutrition, China Medical University, Taichung, Taiwan.
AD  - Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, 
      Taiwan.
FAU - Chen, Chih-Chieh
AU  - Chen CC
AD  - Department of Nutrition, China Medical University, Taichung, Taiwan.
FAU - Li, Chien-Chun
AU  - Li CC
AD  - Department of Nutrition, Chung Shan Medical University, Taichung, Taiwan.
AD  - Department of Nutrition, Chung Shan Medical University Hospital, Taichung, 
      Taiwan.
FAU - Tseng, Meng-Hsien
AU  - Tseng MH
AD  - Department of Nutrition, China Medical University, Taichung, Taiwan.
FAU - Lo, Chia-Wen
AU  - Lo CW
AD  - Department of Nutrition, China Medical University, Taichung, Taiwan.
FAU - Liu, Kai-Li
AU  - Liu KL
AD  - Department of Nutrition, Chung Shan Medical University, Taichung, Taiwan.
AD  - Department of Nutrition, Chung Shan Medical University Hospital, Taichung, 
      Taiwan.
FAU - Yang, Ya-Chen
AU  - Yang YC
AD  - Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, 
      Taiwan.
FAU - Chen, Haw-Wen
AU  - Chen HW
AD  - Department of Nutrition, China Medical University, Taichung, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20221125
PL  - Singapore
TA  - Am J Chin Med
JT  - The American journal of Chinese medicine
JID - 7901431
RN  - 0 (Inflammasomes)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 410105JHGR (andrographolide)
RN  - 0 (Lipopolysaccharides)
RN  - EC 1.11.1.6 (Catalase)
RN  - 0 (Antioxidants)
RN  - 0 (Interleukin-1)
RN  - 0 (Nlrp3 protein, mouse)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Inflammasomes/metabolism
MH  - *NLR Family, Pyrin Domain-Containing 3 Protein/genetics/metabolism
MH  - Lipopolysaccharides/adverse effects
MH  - Catalase/metabolism
MH  - Macrophages/metabolism
MH  - Inflammation/metabolism
MH  - Antioxidants/metabolism
MH  - Interleukin-1/metabolism
MH  - Mice, Inbred C57BL
OTO - NOTNLM
OT  - Andrographolide (AND)
OT  - Autophagy
OT  - Bone Marrow-Derived Macrophages (BMDMs)
OT  - NLRP3 Inflammasome
OT  - Palmitic Acid (PA)
EDAT- 2022/11/25 06:00
MHDA- 2023/02/01 06:00
CRDT- 2022/11/24 00:52
PHST- 2022/11/25 06:00 [pubmed]
PHST- 2023/02/01 06:00 [medline]
PHST- 2022/11/24 00:52 [entrez]
AID - 10.1142/S0192415X23500088 [doi]
PST - ppublish
SO  - Am J Chin Med. 2023;51(1):129-147. doi: 10.1142/S0192415X23500088. Epub 2022 Nov 
      25.