PMID- 36420482
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221125
IS  - 2667-0119 (Electronic)
IS  - 2667-0119 (Linking)
VI  - 2
DP  - 2021 Dec
TI  - Immunological characteristics of dendritic cells marker CD83 in flounder 
      (Paralichthys olivaceus).
PG  - 100030
LID - 10.1016/j.fsirep.2021.100030 [doi]
LID - 100030
AB  - Dendritic cells (DCs) are the professional antigen presenting cells, which play 
      important roles in regulating the immune response and tolerance. In mammals, CD83 
      is considered as a maturation marker for DCs, which is a type I membrane 
      glycoprotein. In this research, the gene sequence of the CD83 in Paralichthys 
      olivaceus was obtained on NCBI, the sequence characteristics were studied and 
      CD83 was detected in the head kidney, spleen, gut, gill, liver and muscle, with 
      lower expression in the liver and muscle and higher expression in the head kidney 
      and spleen. And then the CD83 recombinant protein was expressed and the specific 
      antibodies (Abs) were prepared, transiently expressed CD83 eukaryotic proteins 
      could be identified by western blot and indirect immunofluorescence. Then single 
      cell suspensions of head kidney were obtained and DCs were collected by density 
      gradient centrifugation after 7 days of cultivation. Giemsa staining revealed 
      that the nuclei were kidney and dumbbell shaped, and the cell surfaces had 
      dendritic or pseudopod-like protrusions. In addition, the DCs could phagocytose 
      fluorescent microspheres, and the phagocytosis rate was 79.27+/-1.01%. After 
      incubation with allogeneic lymphocytes, it was found that the lymphocytes 
      clustered and proliferated significantly with the ratio ranging from 39.0% to 
      59.8% in a dose-dependent manner, which proved that the DCs could elicit mixed 
      lymphocyte responses. After LPS immunization in vivo, the relative expression of 
      CD83 in the head kidney and spleen showed a trend of increasing and then 
      decreasing, peaking at 6 h. Meanwhile, the expression of CD83, MHC II, CD80/86 
      and CD40 were significantly up-regulated in DCs after stimulated with LPS for 
      24 h in vitro. And then IIF revealed that CD83(+) cells could be detected in the 
      LPS-stimulated group using CD83 Abs, while no positive signal was detected in the 
      control group, which suggested that CD83 protein may be considered as a specific 
      marker for the maturation of DCs in teleost.
CI  - (c) 2021 The Author(s).
FAU - Dong, Fujing
AU  - Dong F
AD  - Laboratory of pathology and Immunology of Aquatic Animals, KLMME, Ocean 
      University of China, Qingdao, 266003, China.
FAU - Song, Xiangdi
AU  - Song X
AD  - Laboratory of pathology and Immunology of Aquatic Animals, KLMME, Ocean 
      University of China, Qingdao, 266003, China.
FAU - Xing, Jing
AU  - Xing J
AD  - Laboratory of pathology and Immunology of Aquatic Animals, KLMME, Ocean 
      University of China, Qingdao, 266003, China.
AD  - Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 
      National Laboratory for Marine Science and Technology, Qingdao 266071, China.
FAU - Tang, Xiaoqian
AU  - Tang X
AD  - Laboratory of pathology and Immunology of Aquatic Animals, KLMME, Ocean 
      University of China, Qingdao, 266003, China.
FAU - Sheng, Xiuzhen
AU  - Sheng X
AD  - Laboratory of pathology and Immunology of Aquatic Animals, KLMME, Ocean 
      University of China, Qingdao, 266003, China.
FAU - Chi, Heng
AU  - Chi H
AD  - Laboratory of pathology and Immunology of Aquatic Animals, KLMME, Ocean 
      University of China, Qingdao, 266003, China.
FAU - Zhan, Wenbin
AU  - Zhan W
AD  - Laboratory of pathology and Immunology of Aquatic Animals, KLMME, Ocean 
      University of China, Qingdao, 266003, China.
AD  - Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 
      National Laboratory for Marine Science and Technology, Qingdao 266071, China.
LA  - eng
PT  - Journal Article
DEP - 20211011
PL  - England
TA  - Fish Shellfish Immunol Rep
JT  - Fish and shellfish immunology reports
JID - 9918486785306676
PMC - PMC9680054
OTO - NOTNLM
OT  - CD83
OT  - Dendritic cells
OT  - Immunological characteristic
OT  - Paralichthys olivaceus
OT  - Tissue distribution
COIS- The authors state that the study was conducted without any commercial and 
      financial relationships, which can be interpreted as potential conflicts of 
      interest.
EDAT- 2022/11/25 06:00
MHDA- 2022/11/25 06:01
PMCR- 2021/10/11
CRDT- 2022/11/24 02:41
PHST- 2021/08/31 00:00 [received]
PHST- 2021/10/08 00:00 [revised]
PHST- 2021/10/08 00:00 [accepted]
PHST- 2022/11/24 02:41 [entrez]
PHST- 2022/11/25 06:00 [pubmed]
PHST- 2022/11/25 06:01 [medline]
PHST- 2021/10/11 00:00 [pmc-release]
AID - S2667-0119(21)00025-6 [pii]
AID - 100030 [pii]
AID - 10.1016/j.fsirep.2021.100030 [doi]
PST - epublish
SO  - Fish Shellfish Immunol Rep. 2021 Oct 11;2:100030. doi: 
      10.1016/j.fsirep.2021.100030. eCollection 2021 Dec.