PMID- 36422056
OWN - NLM
STAT- MEDLINE
DCOM- 20230202
LR  - 20230202
IS  - 1097-0339 (Electronic)
IS  - 1097-0339 (Linking)
VI  - 51
IP  - 3
DP  - 2023 Mar
TI  - A novel method for making cell blocks with higher cellular yield.
PG  - 182-190
LID - 10.1002/dc.25081 [doi]
AB  - INTRODUCTION: Cytopathology is an important part of pathology that is used to 
      diagnose disease on the cellular level. The application of the cell block (CB) 
      technique plays a vital role in cytological diagnosis, as blocks and slides can 
      be further used for special stains, immunohistochemistry (IHC), and molecular 
      pathological analysis. Several methods for making CBs have been reported, but 
      their procedures and cellular yield are still deemed unsatisfactory. In this 
      article, we used gellan gum (GG) as an adjuvant for CBs, which resulted in higher 
      cellular yield with simpler procedures. METHODS: CBs were prepared by using GG, 
      copper sulfate, plasma/thrombin, or pregelatinized starch methods. The procedures 
      of each of these four methods were then compared. CB sections were stained with 
      hematoxylin and eosin (H&E), and the background and morphological features seen 
      by H&E staining were compared. A preliminary IHC and fluorescence in situ 
      hybridization (FISH) study was performed using cytology specimens from eleven and 
      five cases, respectively. The expression of immunocomplex by IHC and the 
      molecular signals detected by FISH were compared in CB sections made by the four 
      methods and a section derived from the biopsy specimen block from the same 
      patient. Feulgen staining, Alcian blue staining, and Masson trichrome staining 
      were performed on the CB sections from 3 cases of pleural fluid. The cellular 
      yield of CB sections from 83 cases according to the four methods was compared 
      using NDP analysis software. RESULTS: The results demonstrated that sections 
      derived from CBs made with GG had a clear background and good morphological 
      features by H&E staining. The expression of immunocomplex by IHC and the 
      molecular signals of FISH detection in the sections from CBs made by GG were 
      accurately located just as those in biopsy sections from the same patient. The 
      DNA, acidic mucus, and fibrin could be clearly identified through special stains 
      in the CB sections. The procedures involved in the GG method were easily 
      controllable and the coagulated gel increased the ease by which the CB was 
      embedded and sectioned. Specifically, sections from CBs made by the GG method 
      contained higher cellular yield because cells could be concentrated on the bottom 
      of the gel after centrifugation. CONCLUSION: This novel method for making CBs is 
      a practical, simple method that can result in higher cellular yield. This method 
      is therefore worth promoting in clinical applications.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Liu, Hongying
AU  - Liu H
AD  - Department of Pathology, The Second Clinical College of Guangzhou University of 
      Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 
      China.
FAU - Huang, Zhendong
AU  - Huang Z
AD  - Department of Pathology, The Second Clinical College of Guangzhou University of 
      Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 
      China.
FAU - Li, Weiping
AU  - Li W
AD  - Department of Pathology, The Second Clinical College of Guangzhou University of 
      Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 
      China.
FAU - Cao, Zhuo
AU  - Cao Z
AD  - Department of Pathology, The Second Clinical College of Guangzhou University of 
      Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 
      China.
FAU - Luo, Biyi
AU  - Luo B
AD  - Department of Pathology, The Second Clinical College of Guangzhou University of 
      Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 
      China.
FAU - Peng, Yan
AU  - Peng Y
AD  - Department of Pathology, The Second Clinical College of Guangzhou University of 
      Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 
      China.
FAU - Liu, Ying
AU  - Liu Y
AD  - Department of Pathology, The Second Clinical College of Guangzhou University of 
      Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 
      China.
FAU - Zheng, Guangjuan
AU  - Zheng G
AD  - Department of Pathology, The Second Clinical College of Guangzhou University of 
      Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 
      China.
FAU - He, Qinglian
AU  - He Q
AUID- ORCID: 0000-0002-8046-4539
AD  - Department of Pathology, The Second Clinical College of Guangzhou University of 
      Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20221124
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
SB  - IM
MH  - Humans
MH  - *Cytodiagnosis/methods
MH  - In Situ Hybridization, Fluorescence
MH  - Immunohistochemistry
MH  - Biopsy
OTO - NOTNLM
OT  - cell block
OT  - cellular yield
OT  - cytological specimens
OT  - cytology
OT  - gellan gum
EDAT- 2022/11/25 06:00
MHDA- 2023/02/03 06:00
CRDT- 2022/11/24 10:23
PHST- 2022/10/25 00:00 [revised]
PHST- 2022/07/31 00:00 [received]
PHST- 2022/11/09 00:00 [accepted]
PHST- 2022/11/25 06:00 [pubmed]
PHST- 2023/02/03 06:00 [medline]
PHST- 2022/11/24 10:23 [entrez]
AID - 10.1002/dc.25081 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2023 Mar;51(3):182-190. doi: 10.1002/dc.25081. Epub 2022 Nov 
      24.