PMID- 36423098
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221128
IS  - 2306-7381 (Electronic)
IS  - 2306-7381 (Linking)
VI  - 9
IP  - 11
DP  - 2022 Nov 21
TI  - PPARgamma/mTOR Regulates the Synthesis and Release of Prostaglandins in Ovine 
      Trophoblast Cells in Early Pregnancy.
LID - 10.3390/vetsci9110649 [doi]
LID - 649
AB  - Trophoblast cells synthesize and secrete prostaglandins (PGs), which are 
      essential for ruminants in early gestation to recognize pregnancy. Hormones in 
      the intrauterine environment play an important role in regulating PGs synthesis 
      during implantation, but the underlying mechanism remains unclear. In this study, 
      co-treatment of sheep trophoblast cells (STCs) with progesterone (P(4)), 
      estradiol (E(2)), and interferon-tau (IFN-tau) increased the ratio of prostaglandin 
      E2 (PGE(2)) to prostaglandin F2alpha (PGF(2alpha)) and upregulated peroxisome 
      proliferator-activated receptor gamma (PPARgamma) expression, while inhibiting the 
      mechanistic target of rapamycin (mTOR) pathway and activating cellular autophagy. 
      Under hormone treatment, inhibition of PPARgamma activity decreased the ratio of 
      PGE(2)/PGF2alpha and cellular activity, while activating expression of the mTOR 
      downstream marker-the phosphorylation of p70S6K (p-p70S6K). We also found that 
      the PPARgamma/mTOR pathway played an important role in regulating trophoblast cell 
      function. Inhibition of the mTOR pathway by rapamycin increased the ratio of 
      PGE(2)/PGF(2alpha) and decreased the expression of apoptosis-related proteins after 
      inhibiting PPARgamma activity. In conclusion, our findings provide new insights into 
      the molecular mechanism of prostaglandin regulation of trophoblast cells in sheep 
      during early pregnancy, indicating that the PPARgamma/mTOR pathway plays an important 
      role in PGs secretion and cell viability.
FAU - Hao, Kexing
AU  - Hao K
AD  - College of Animal Science and Technology, Shihezi University, Shihezi 832000, 
      China.
FAU - Wang, Jing
AU  - Wang J
AD  - College of Animal Science and Technology, Shihezi University, Shihezi 832000, 
      China.
FAU - Li, Zhiyuan
AU  - Li Z
AD  - College of Animal Science and Technology, Shihezi University, Shihezi 832000, 
      China.
FAU - Chen, Huihui
AU  - Chen H
AD  - College of Animal Science and Technology, Shihezi University, Shihezi 832000, 
      China.
FAU - Jia, Bin
AU  - Jia B
AD  - College of Animal Science and Technology, Shihezi University, Shihezi 832000, 
      China.
FAU - Hu, Guangdong
AU  - Hu G
AD  - College of Animal Science and Technology, Shihezi University, Shihezi 832000, 
      China.
LA  - eng
GR  - 32060751/National Natural Science Foundation of China/
GR  - 32160833/National Natural Science Foundation of China/
GR  - MYSKLKF201905/Open Project of State Key Laboratory of Sheep Genetic Improvement 
      and Healthy Production/
GR  - 2020BTDJ05/Open Project of Key Laboratory of Animal Disease Prevention and 
      Control Corps/
PT  - Journal Article
DEP - 20221121
PL  - Switzerland
TA  - Vet Sci
JT  - Veterinary sciences
JID - 101680127
PMC - PMC9694237
OTO - NOTNLM
OT  - PGE2/PGF2alpha
OT  - PPARgamma
OT  - mTOR
OT  - prostaglandins
OT  - trophoblast cells
COIS- The authors declare no conflict of interest.
EDAT- 2022/11/25 06:00
MHDA- 2022/11/25 06:01
PMCR- 2022/11/21
CRDT- 2022/11/24 12:03
PHST- 2022/10/12 00:00 [received]
PHST- 2022/11/04 00:00 [revised]
PHST- 2022/11/17 00:00 [accepted]
PHST- 2022/11/24 12:03 [entrez]
PHST- 2022/11/25 06:00 [pubmed]
PHST- 2022/11/25 06:01 [medline]
PHST- 2022/11/21 00:00 [pmc-release]
AID - vetsci9110649 [pii]
AID - vetsci-09-00649 [pii]
AID - 10.3390/vetsci9110649 [doi]
PST - epublish
SO  - Vet Sci. 2022 Nov 21;9(11):649. doi: 10.3390/vetsci9110649.