PMID- 36423715
OWN - NLM
STAT- MEDLINE
DCOM- 20221220
LR  - 20221221
IS  - 1879-3169 (Electronic)
IS  - 0378-4274 (Linking)
VI  - 373
DP  - 2023 Jan 15
TI  - Effect of trichloroethanol on TLR2 and TLR4/NF-kappaB-mediated antigen processing and 
      presentation in HLA-B* 13:01-transfected antigen-presenting cells.
PG  - 123-131
LID - S0378-4274(22)01793-3 [pii]
LID - 10.1016/j.toxlet.2022.11.009 [doi]
AB  - Trichloroethanol (TCOH), as a metabolite of trichloroethylene, has sensitization 
      in the pathogenesis of trichloroethylene-induced hypersensitivity dermatitis 
      (TIHD) which the human leukocyte antigen (HLA)-B *13:01 gene is strongly 
      associated with it. However, it is still obscure how TCOH participates in the 
      pathogenesis of TIHD. Here, we demonstrate that TLR2 and TLR4 signaling through 
      MyD88 and TRAF6-dependent pathway could activate NF-kappaB by promoting degradation 
      of the inhibitor IkappaB-alpha to stimulate the process of NF-kappaB nuclear translocation. 
      Besides, the crucial molecules of antigen processing and presentation, including 
      TAP1, LMP2, LMP7, and HLA-B* 13:01, were all enhanced and the abundance of 
      HLA-B* 13:01 on the surface of CIR-B* 13:01 cells was also up-regulated with the 
      TCOH concentration increasing. Notably, we used 50 muM pyrrolidinedithiocarbamate 
      (ammonium) to effectively inhibit the activation of NF-kappaB, which could 
      effectively reverse the stimulation of antigen processing and presentation in 
      TCOH-treated CIR-B* 13:01 cells. Taken together, we speculated that TCOH could 
      promote the abundance of HLA complex on the antigen-presenting cells via TLR2 and 
      TLR4/NF-kappaB to induce the severe reactivation of T lymphocytes, leading to the 
      extreme immune response.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Yi, Mengnan
AU  - Yi M
AD  - Key Laboratory of Chemical Safety and Health, National Institute for Occupational 
      Health and Poison Control, Chinese Center for Disease Control and Prevention, 
      Beijing 100050, China.
FAU - Liu, Shuai
AU  - Liu S
AD  - Key Laboratory of Chemical Safety and Health, National Institute for Occupational 
      Health and Poison Control, Chinese Center for Disease Control and Prevention, 
      Beijing 100050, China.
FAU - Jiao, Bo
AU  - Jiao B
AD  - Key Laboratory of Chemical Safety and Health, National Institute for Occupational 
      Health and Poison Control, Chinese Center for Disease Control and Prevention, 
      Beijing 100050, China.
FAU - Niu, Yong
AU  - Niu Y
AD  - Key Laboratory of Chemical Safety and Health, National Institute for Occupational 
      Health and Poison Control, Chinese Center for Disease Control and Prevention, 
      Beijing 100050, China.
FAU - Shen, Meili
AU  - Shen M
AD  - Key Laboratory of Chemical Safety and Health, National Institute for Occupational 
      Health and Poison Control, Chinese Center for Disease Control and Prevention, 
      Beijing 100050, China.
FAU - Duan, Huawei
AU  - Duan H
AD  - Key Laboratory of Chemical Safety and Health, National Institute for Occupational 
      Health and Poison Control, Chinese Center for Disease Control and Prevention, 
      Beijing 100050, China.
FAU - Dai, Yufei
AU  - Dai Y
AD  - Key Laboratory of Chemical Safety and Health, National Institute for Occupational 
      Health and Poison Control, Chinese Center for Disease Control and Prevention, 
      Beijing 100050, China. Electronic address: daiyf@niohp.chinacdc.cn.
LA  - eng
PT  - Journal Article
DEP - 20221121
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (NF-kappa B)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - AW835AJ62N (2,2,2-trichloroethanol)
RN  - 290YE8AR51 (Trichloroethylene)
RN  - 0 (HLA-B Antigens)
RN  - 0 (TLR2 protein, human)
RN  - 0 (TLR4 protein, human)
SB  - IM
MH  - Humans
MH  - *NF-kappa B/metabolism
MH  - Toll-Like Receptor 2/genetics/metabolism
MH  - Toll-Like Receptor 4/genetics/metabolism
MH  - Antigen Presentation
MH  - *Trichloroethylene
MH  - Antigen-Presenting Cells/metabolism
MH  - HLA-B Antigens
OTO - NOTNLM
OT  - Antigen processing and presentation
OT  - HLA-B* 13:01
OT  - TIHD
OT  - TLRs/NF-kappaB
OT  - Trichloroethanol
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/11/25 06:00
MHDA- 2022/12/21 06:00
CRDT- 2022/11/24 19:24
PHST- 2022/07/04 00:00 [received]
PHST- 2022/10/28 00:00 [revised]
PHST- 2022/11/17 00:00 [accepted]
PHST- 2022/11/25 06:00 [pubmed]
PHST- 2022/12/21 06:00 [medline]
PHST- 2022/11/24 19:24 [entrez]
AID - S0378-4274(22)01793-3 [pii]
AID - 10.1016/j.toxlet.2022.11.009 [doi]
PST - ppublish
SO  - Toxicol Lett. 2023 Jan 15;373:123-131. doi: 10.1016/j.toxlet.2022.11.009. Epub 
      2022 Nov 21.