PMID- 36424458
OWN - NLM
STAT- MEDLINE
DCOM- 20230110
LR  - 20230209
IS  - 1096-9071 (Electronic)
IS  - 0146-6615 (Linking)
VI  - 95
IP  - 1
DP  - 2023 Jan
TI  - Development of an HiBiT-tagged reporter H3N2 influenza A virus and its utility as 
      an antiviral screening platform.
PG  - e28345
LID - 10.1002/jmv.28345 [doi]
AB  - The balance of the segmented genome derived from naturally occurring influenza A 
      viruses (IAVs) is delicate and vulnerable to foreign insertions, thus most 
      reporter IAVs up to date are generated using the backbone of the 
      laboratory-adapted strains. In this study, we constructed a reporter influenza 
      A/H3N2 virus (A/NY-HiBiT) which was derived from a clinical isolate, by placing a 
      minimized HiBiT tag to the N-terminus of the viral nuclear-export protein (NEP). 
      Here, we show that this 11-amino acid HiBiT tag did not adversely impact the 
      viral genome balance, and the recombinant A/NY-HiBiT virus maintains its relative 
      stability. Moreover, the replication profile of the HiBiT-tagged virus can be 
      measured by a simple Nano-Glo assay, providing a robust high-throughput screening 
      (THS) platform. We used this platform to evaluate a collection of the 
      pre-purified fractions which were derived from rare Chinese medicinal materials, 
      and we identified three fractions, including wild Trametes robiniophila (50% 
      methanol fraction), Ganoderma (water fraction), and wild Phellinus igniarius 
      (ethyl acetate fraction), as potent anti-IAV actives. Our results demonstrate 
      that this IAV reporter can be used as a powerful HTS platform for antiviral 
      development.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Zhang, Chengcheng
AU  - Zhang C
AD  - College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 
      Shandong, China.
FAU - Zhang, Guoying
AU  - Zhang G
AD  - College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 
      Shandong, China.
FAU - Zhang, Yu
AU  - Zhang Y
AD  - College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 
      Shandong, China.
FAU - Lin, Xiaojing
AU  - Lin X
AD  - College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 
      Shandong, China.
FAU - Zhao, Xiujuan
AU  - Zhao X
AD  - Innovation Research Institute of Traditional Chinese Medicine, Shandong 
      University of Traditional Chinese Medicine, Jinan, Shandong, China.
FAU - Cui, Qinghua
AU  - Cui Q
AUID- ORCID: 0000-0002-4696-3254
AD  - Innovation Research Institute of Traditional Chinese Medicine, Shandong 
      University of Traditional Chinese Medicine, Jinan, Shandong, China.
AD  - Qingdao Academy of Chinese Medicinal Sciences, Shandong University of Traditional 
      Chinese Medicine, Qingdao, China.
FAU - Rong, Lijun
AU  - Rong L
AD  - Department of Microbiology and Immunology, College of Medicine, University of 
      Illinois at Chicago, Chicago, Illinois, USA.
FAU - Du, Ruikun
AU  - Du R
AUID- ORCID: 0000-0002-9172-7824
AD  - Innovation Research Institute of Traditional Chinese Medicine, Shandong 
      University of Traditional Chinese Medicine, Jinan, Shandong, China.
AD  - Qingdao Academy of Chinese Medicinal Sciences, Shandong University of Traditional 
      Chinese Medicine, Qingdao, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (Antiviral Agents)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Humans
MH  - *Influenza A virus/genetics
MH  - Influenza A Virus, H3N2 Subtype/genetics/metabolism
MH  - Antiviral Agents/pharmacology/metabolism
MH  - Trametes/metabolism
MH  - *Influenza, Human/genetics
MH  - Viral Proteins/genetics
MH  - Virus Replication
OTO - NOTNLM
OT  - H3N2 subtype
OT  - HiBiT tag
OT  - antiviral screen
OT  - influenza A virus
OT  - reporter virus
EDAT- 2022/11/25 06:00
MHDA- 2023/01/11 06:00
CRDT- 2022/11/24 23:27
PHST- 2022/11/18 00:00 [revised]
PHST- 2022/10/25 00:00 [received]
PHST- 2022/11/21 00:00 [accepted]
PHST- 2022/11/25 06:00 [pubmed]
PHST- 2023/01/11 06:00 [medline]
PHST- 2022/11/24 23:27 [entrez]
AID - 10.1002/jmv.28345 [doi]
PST - ppublish
SO  - J Med Virol. 2023 Jan;95(1):e28345. doi: 10.1002/jmv.28345.