PMID- 36426398 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20221217 IS - 2092-7355 (Print) IS - 2092-7363 (Electronic) IS - 2092-7355 (Linking) VI - 14 IP - 6 DP - 2022 Nov TI - Altered Expression of Substance P and NK1R in CCR3(+) and CD123(+)HLA-DR(-) Basophils Under Airway Allergic Conditions. PG - 687-712 LID - 10.4168/aair.2022.14.6.687 [doi] AB - PURPOSE: To explore expression of SP and NK1R in basophils of allergic asthma (AA), allergic rhinitis (AR) and AR combined with AA (ARA), and influence of allergens and immunoglobulin E (IgE) mediated mechanisms on SP and NK1R expression. METHODS: Expression of SP and NK1R was detected by flow cytometry, NK1R mRNA expression was detected by real time quantitative polymerase chain reaction (qPCR), and mouse AR and AA models were employed for in vivo study. RESULTS: SP(+) and NK1R(+) cells increased in CCR3(+) and CD123(+)HLA-DR(-) granulocytes of AA. PPE elevated proportions of SP(+) cells in CCR3(+) and CD123(+)HLA-DR(-) granulocytes, whereas ASWE and HDME augmented SP(+) cells in CD123(+)HLA-DR(-) granulocytes of AR and ARA patients. ASWE, HDME and PPE increased proportions of NK1R(+) cells in CCR3(+) PBMC and CD123(+)HLA-DR(-) granulocytes of AR patients. OVA, Der p1, IL-33, IL-37, IgE and SP enhanced NK1R expression on KU812 cells. NK1R expressing basophils were increased in blood of OVA sensitized and challenged AR and AA mice. FcepsilonRI-KO AA mice seemed to have less NK1R(+) basophils than WT AA mice in their blood. CONCLUSION: CCR3(+) and CD123(+)HLA-DR(-) cells are likely involved in AA and AR via SP and NK1R. IgE-related mechanism may participate in upregulation of NK1R expression. CI - Copyright (c) 2022 The Korean Academy of Asthma, Allergy and Clinical Immunology * The Korean Academy of Pediatric Allergy and Respiratory Disease. FAU - Geng, Shiyang AU - Geng S AUID- ORCID: 0000-0002-0931-743X AD - Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China. FAU - Xie, Hua AU - Xie H AUID- ORCID: 0000-0003-4904-0698 AD - Department of the PLA Center of Respiratory and Allergic Disease Diagnosing Management, General Hospital of Northern Theater Command, Shenyang, China. FAU - Chen, Liping AU - Chen L AUID- ORCID: 0000-0003-0285-4897 AD - Translational Medicine Research Centre, Shenyang Medical College, Shenyang, China. FAU - Chen, Dong AU - Chen D AUID- ORCID: 0000-0002-9260-4455 AD - Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China. AD - Department of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China. FAU - Lu, Sijing AU - Lu S AUID- ORCID: 0000-0002-5781-0958 AD - Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China. AD - Department of Respiration, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China. FAU - Zhao, Nan AU - Zhao N AUID- ORCID: 0000-0002-6558-7506 AD - Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China. FAU - Yang, Ruiming AU - Yang R AUID- ORCID: 0000-0002-5513-5397 AD - Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China. FAU - Wang, Zhao AU - Wang Z AUID- ORCID: 0000-0002-6819-4484 AD - Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China. FAU - He, Shaoheng AU - He S AUID- ORCID: 0000-0003-4760-4816 AD - Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China. AD - Translational Medicine Research Centre, Shenyang Medical College, Shenyang, China. shoahenghe@126.com. FAU - Zhang, Huiyun AU - Zhang H AUID- ORCID: 0000-0003-0631-3000 AD - Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China. AD - Translational Medicine Research Centre, Shenyang Medical College, Shenyang, China. zhanghuiyun2003@126.com. LA - eng GR - 19-109-4-17/Shenyang Science and Technology Plan Project/China GR - 2018-30/Key Laboratory of Research on Pathogenesis of Allergen provoked Allergic Disease, Liaoning Province/China GR - 2018-38/Climbing Scholar Project in Liaoning province/China GR - Z17-5-044/Science and Technology Innovation "double hundred project" in Shenyang/China GR - 2017CXCY-C-01/Innovation and Entrepreneurship Projects for High-Level Talent in Shenyang/China GR - 2017-2020/Leader Team Project in Jinzhou Medical University/China GR - 2021JH2/10300022/Liaoning Province Science and Technology Planning project/China GR - 2019JH8/10300053/Liaoning Province Science and Technology Planning project/China GR - LJKZ0796/Natural Science Foundation of Liaoning Province/China GR - 2019-ZD-0801/Natural Science Foundation of Liaoning Province/China GR - JYBZQT2110/Boze Project of Jinzhou Medical University/China PT - Journal Article PL - Korea (South) TA - Allergy Asthma Immunol Res JT - Allergy, asthma & immunology research JID - 101518382 PMC - PMC9709683 OTO - NOTNLM OT - IgE OT - Substance P OT - allergen OT - allergy OT - asthma OT - neurokinin 1 receptor OT - rhinitis COIS- Shaoheng He is a cofounder and employee of Huiallergy Co. LTD. The other authors declare no competing interests. EDAT- 2022/11/26 06:00 MHDA- 2022/11/26 06:01 PMCR- 2022/11/01 CRDT- 2022/11/25 03:05 PHST- 2022/02/24 00:00 [received] PHST- 2022/08/11 00:00 [revised] PHST- 2022/08/16 00:00 [accepted] PHST- 2022/11/25 03:05 [entrez] PHST- 2022/11/26 06:00 [pubmed] PHST- 2022/11/26 06:01 [medline] PHST- 2022/11/01 00:00 [pmc-release] AID - 14.687 [pii] AID - 10.4168/aair.2022.14.6.687 [doi] PST - ppublish SO - Allergy Asthma Immunol Res. 2022 Nov;14(6):687-712. doi: 10.4168/aair.2022.14.6.687.