PMID- 36428408 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20230308 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 12 IP - 22 DP - 2022 Nov 17 TI - Does Antioxidant Mitoquinone (MitoQ) Ameliorate Oxidative Stress in Frozen-Thawed Rooster Sperm? LID - 10.3390/ani12223181 [doi] LID - 3181 AB - In this study, we aimed to determine the benefit of mitoquinone (MitoQ) in rooster semen extenders on sperm quality, motility parameters, antioxidant capacities, and apoptotic changes in post-thawed rooster semen. A total of 85 ejaculates from 18 roosters were collected and then divided into five equal aliquots and cryopreserved in extenders with 1.0% soy lecithin nanoparticles that contained various concentrations of MitoQ (0 nM (M0), 50 nM (M50), 100 nM (M100), 150 nM (M150), and 200 nM (M200)). By using a computer-assisted semen analyzer, sperm motility parameters were assessed after freeze thawing. The M150 group had significantly higher percentages of total motility, progressive motility, viability, acrosome membrane integrity, and mitochondrial activity than the other groups (p < 0.05). Compared to other groups, M100 and M150 groups produced a higher percentage of plasma membrane integrity and ATP contents (p < 0.05). Additionally, the lowest levels of ROS and MDA in spermatozoa were observed in M150 group (p < 0.05), whereas the highest levels of ROS and MDA were observed in sperm in the controls or the M200 group (p < 0.05). Significantly higher values of SOD, GPx, and Cas-3 were found in the M150 group compared to other groups (p < 0.05). Overall, these results demonstrate that MitoQ at 150 nM not only ameliorates post-thawed sperm quality and motility parameters by restoring ATP levels and preventing membrane damage, but also improves redox balance and antiapoptotic activities. FAU - Sun, Lingwei AU - Sun L AUID- ORCID: 0000-0002-3438-6584 AD - Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China. AD - Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai 201106, China. AD - Key Laboratory of Livestock and Poultry Resources (Pig) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai 201106, China. FAU - He, Mengqian AU - He M AD - Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China. AD - Key Laboratory of Livestock and Poultry Resources (Pig) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai 201106, China. FAU - Xu, Jiehuan AU - Xu J AD - Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China. AD - Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai 201106, China. FAU - Wu, Caifeng AU - Wu C AD - Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China. AD - Key Laboratory of Livestock and Poultry Resources (Pig) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai 201106, China. FAU - Zhang, Shushan AU - Zhang S AD - Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China. AD - Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai 201106, China. FAU - Zhang, Defu AU - Zhang D AD - Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China. AD - Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai 201106, China. AD - Key Laboratory of Livestock and Poultry Resources (Pig) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai 201106, China. FAU - Dai, Jianjun AU - Dai J AD - Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China. AD - Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai 201106, China. AD - Key Laboratory of Livestock and Poultry Resources (Pig) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai 201106, China. FAU - Gao, Jun AU - Gao J AUID- ORCID: 0000-0002-2372-4445 AD - Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China. AD - Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai 201106, China. AD - Key Laboratory of Livestock and Poultry Resources (Pig) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai 201106, China. LA - eng GR - 2022-02-08-00-12-F01197/Shanghai Agriculture Applied Technology Development Program/ GR - 2021YFD1200301/National Key Research and Development Plan/ PT - Journal Article DEP - 20221117 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC9686742 OTO - NOTNLM OT - antioxidant OT - apoptotic sperm OT - cryopreservation OT - mitoquinone (MitoQ) OT - rooster semen COIS- The authors declare no conflict of interest. EDAT- 2022/11/27 06:00 MHDA- 2022/11/27 06:01 PMCR- 2022/11/17 CRDT- 2022/11/26 01:03 PHST- 2022/10/07 00:00 [received] PHST- 2022/11/14 00:00 [revised] PHST- 2022/11/15 00:00 [accepted] PHST- 2022/11/26 01:03 [entrez] PHST- 2022/11/27 06:00 [pubmed] PHST- 2022/11/27 06:01 [medline] PHST- 2022/11/17 00:00 [pmc-release] AID - ani12223181 [pii] AID - animals-12-03181 [pii] AID - 10.3390/ani12223181 [doi] PST - epublish SO - Animals (Basel). 2022 Nov 17;12(22):3181. doi: 10.3390/ani12223181.