PMID- 36431839
OWN - NLM
STAT- MEDLINE
DCOM- 20221129
LR  - 20221213
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 27
IP  - 22
DP  - 2022 Nov 10
TI  - Antioxidant Effects of Roasted Licorice in a Zebrafish Model and Its Mechanisms.
LID - 10.3390/molecules27227743 [doi]
LID - 7743
AB  - Licorice (Gan-Cao, licorice) is a natural antioxidant and roasted licorice is the 
      most common processing specification used in traditional Chinese medicine 
      prescriptions. Traditional Chinese medicine theory deems that the honey-roasting 
      process can promote the efficacy of licorice, including tonifying the spleen and 
      augmenting "Qi" (energy). The antioxidant activity and mechanisms underlying 
      roasted licorice have not yet been reported. In this study, we found that roasted 
      licorice could relieve the oxidative stress injury induced by metronidazole (MTZ) 
      and could restrain the production of excessive reactive oxygen species (ROS) 
      induced by 2,2'-azobis (2-methylpropionamidine) dihydrochloride (AAPH) in a 
      zebrafish model. It was further found that roasted licorice could exert its 
      oxidative activity by upregulating the expression of key genes such as heme 
      oxygenase 1 (HO-1), NAD(P)H quinone dehydrogenase 1 (NQO1), glutamate-cysteine 
      ligase modifier subunit (GCLM), and glutamate-cysteine ligase catalytic subunit 
      (GCLC) in the nuclear factor erythroid 2-related factor 2 (NRF2) signaling 
      pathway both in vivo and in vitro. Furthermore, consistent results were obtained 
      showing that rat serum containing roasted licorice was estimated to reduce cell 
      apoptosis induced by H(2)O(2). Then, the UHPLC-Q-Exactive Orbitrap MS analysis 
      results elucidated the chemical composition of rat plasma containing roasted 
      licorice extracts, including ten prototype chemical components and five metabolic 
      components. Among them, six compounds were found to have binding activity with 
      Kelch-like ECH-associated protein 1 (KEAP1), which plays a crucial role in the 
      transcriptional activity of NRF2, using a molecular docking simulation. The 
      results also showed that liquiritigenin had the strongest binding ability with 
      KEAP1. Immunofluorescence further confirmed that liquiritigenin could induce the 
      nuclear translocation of NRF2. In summary, this study provides a better 
      understanding of the antioxidant effect and mechanisms of roasted licorice, and 
      lays a theoretical foundation for the development of a potential antioxidant for 
      use in clinical practice.
FAU - Zhou, Qian
AU  - Zhou Q
AD  - Shandong Academy of Chinese Medicine, Jinan 250014, China.
FAU - Zhang, Shanshan
AU  - Zhang S
AD  - Shandong Academy of Sciences, Qilu University of Technology, Jinan 250103, China.
FAU - Geng, Xue
AU  - Geng X
AD  - Shandong Institute for Food and Drug Control, Jinan 250101, China.
AD  - School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 
      250355, China.
FAU - Jiang, Haiqiang
AU  - Jiang H
AD  - School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 
      250355, China.
FAU - Dai, Yanpeng
AU  - Dai Y
AD  - Shandong Academy of Chinese Medicine, Jinan 250014, China.
FAU - Wang, Ping
AU  - Wang P
AD  - Shandong Academy of Chinese Medicine, Jinan 250014, China.
FAU - Hua, Min
AU  - Hua M
AD  - Shandong Academy of Chinese Medicine, Jinan 250014, China.
AD  - School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 
      250355, China.
FAU - Gao, Qi
AU  - Gao Q
AD  - Shandong Academy of Chinese Medicine, Jinan 250014, China.
FAU - Lang, Shiyue
AU  - Lang S
AD  - School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 
      250355, China.
FAU - Hou, Lijing
AU  - Hou L
AD  - Shandong Academy of Chinese Medicine, Jinan 250014, China.
AD  - School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 
      250355, China.
FAU - Shi, Dianhua
AU  - Shi D
AD  - Shandong Academy of Chinese Medicine, Jinan 250014, China.
FAU - Zhou, Meng
AU  - Zhou M
AD  - The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan 
      250033, China.
LA  - eng
GR  - 81603299 and 81700636/National Natural Science Foundation of China/
GR  - ZR2020KH021/Key Project of Shandong Provincial Natural Science Foundation/
GR  - 2020M672076/China Postdoctoral Science Foundation/
GR  - SDZYY20200101001/Major Scientific and Technological Innovation Projects of 
      Shandong/
GR  - 2019GSF108150/Key R&D project of Shandong Province/
PT  - Journal Article
DEP - 20221110
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Antioxidants)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Kelch-Like ECH-Associated Protein 1)
RN  - EC 6.3.2.2 (Glutamate-Cysteine Ligase)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - 0 (Plant Extracts)
RN  - 0 (Triterpenes)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Glycyrrhiza/chemistry
MH  - Antioxidants/pharmacology/metabolism
MH  - NF-E2-Related Factor 2/metabolism
MH  - Kelch-Like ECH-Associated Protein 1/metabolism
MH  - Zebrafish/metabolism
MH  - Glutamate-Cysteine Ligase/metabolism
MH  - Hydrogen Peroxide/metabolism
MH  - Molecular Docking Simulation
MH  - Plant Extracts
MH  - *Triterpenes
PMC - PMC9694442
OTO - NOTNLM
OT  - KEAP1
OT  - NRF2
OT  - UHPLC-Q-Exactive Orbitrap MS
OT  - antioxidant
OT  - roasted licorice
OT  - zebrafish model
COIS- The authors declare no conflict of interest.
EDAT- 2022/11/27 06:00
MHDA- 2022/11/30 06:00
PMCR- 2022/11/10
CRDT- 2022/11/26 01:26
PHST- 2022/10/12 00:00 [received]
PHST- 2022/11/06 00:00 [revised]
PHST- 2022/11/08 00:00 [accepted]
PHST- 2022/11/26 01:26 [entrez]
PHST- 2022/11/27 06:00 [pubmed]
PHST- 2022/11/30 06:00 [medline]
PHST- 2022/11/10 00:00 [pmc-release]
AID - molecules27227743 [pii]
AID - molecules-27-07743 [pii]
AID - 10.3390/molecules27227743 [doi]
PST - epublish
SO  - Molecules. 2022 Nov 10;27(22):7743. doi: 10.3390/molecules27227743.