PMID- 36434857
OWN - NLM
STAT- MEDLINE
DCOM- 20230110
LR  - 20230111
IS  - 1532-8511 (Electronic)
IS  - 1052-3057 (Linking)
VI  - 32
IP  - 1
DP  - 2023 Jan
TI  - MiR-342-5p protects neurons from cerebral ischemia induced-apoptosis through 
      regulation of Akt/NF-kappaB pathways by targeting CCAR2.
PG  - 106901
LID - S1052-3057(22)00593-6 [pii]
LID - 10.1016/j.jstrokecerebrovasdis.2022.106901 [doi]
AB  - OBJECTIVES: Ischemic stroke causes high morbidity, mortality and health burden in 
      the world. MiR-342-5p was associated with Alzheimer's disease and 
      cardio-protection. Herein, we aimed to reveal effects of miR-342-5p on cerebral 
      ischemia injury as well as novel targets for stroke. MATERIALS AND METHODS: 
      AgomiR-342-5p was intracerebroventricularly injected into the middle cerebral 
      artery occlusion (MCAO) mouse models to evaluate functions of miR-342-5p on 
      cerebral ischemia. RT-qPCR and western blot assays were used to evaluate genes 
      expression. Oxygen-glucose deprivation (OGD) was used as an in vitro model for 
      ischemia. Viability and apoptosis ratio of neurons was evaluated by CCK-8, LDH 
      release detection, and flow cytometry. The potential targets of miR-342-5p were 
      predicted by Targetscan, and their interaction was confirmed by luciferase assay. 
      RESULTS: The intervention of miR-342-5p effectively attenuated ischemic injury in 
      MCAO mice. MiR-342-5p overexpression could protect neurons against OGD-induced 
      injury, as revealed by increased cell viability and BCL2 expression, and 
      decreased LDH release, apoptosis ratio, and BAX expression in OGD-induced 
      neurons. Mechanically, miR-342-5p could directly bound with CCAR2 to inhibit its 
      expression. Overexpressing CARR2 aggravated the OGD-induced injury of neurons, 
      which was partly restrained by overexpressing miR-342-5p reversed. Furthermore, 
      miR-342-5p/CARR2 axis regulates Akt/NF-kappaB signaling pathway in vitro as well as 
      in vivo cerebral ischemia models. CONCLUSIONS: MiR-342-5p inhibited neuron 
      apoptosis by regulating Akt/NF-kB signaling pathway via CCAR2 suppression. Our 
      findings revealed the neuroprotection of miR-342-5p in cerebral ischemia.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Zhu, Haochun
AU  - Zhu H
AD  - Department of Neurology, General Hospital of Hebi Coal Industry Group Co., Ltd., 
      No. 84, Hongqi Street, Hebi, Henan 458000, China. Electronic address: 
      zhc53927185@163.com.
FAU - Zhang, Yanhua
AU  - Zhang Y
AD  - Department of Neurology, General Hospital of Hebi Coal Industry Group Co., Ltd., 
      No. 84, Hongqi Street, Hebi, Henan 458000, China. Electronic address: 
      hnhbzyh@163.com.
FAU - Zhu, Yanling
AU  - Zhu Y
AD  - Department of Neurology, General Hospital of Hebi Coal Industry Group Co., Ltd., 
      No. 84, Hongqi Street, Hebi, Henan 458000, China. Electronic address: 
      zhuyanling9877@163.com.
LA  - eng
PT  - Journal Article
DEP - 20221123
PL  - United States
TA  - J Stroke Cerebrovasc Dis
JT  - Journal of stroke and cerebrovascular diseases : the official journal of National 
      Stroke Association
JID - 9111633
RN  - 0 (NF-kappa B)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - 0 (MicroRNAs)
RN  - S88TT14065 (Oxygen)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Mice
MH  - Animals
MH  - NF-kappa B/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - *Brain Ischemia/genetics/metabolism
MH  - Infarction, Middle Cerebral Artery/genetics/metabolism
MH  - Oxygen/metabolism
MH  - Apoptosis
MH  - Neurons/metabolism
MH  - Glucose
MH  - *Reperfusion Injury/metabolism
OTO - NOTNLM
OT  - Akt/NF-kB
OT  - CCAR2
OT  - MCAO
OT  - MiR-342-5p
OT  - Stroke
COIS- Declaration of Competing Interest The authors declare that they have no competing 
      interests.
EDAT- 2022/11/27 06:00
MHDA- 2023/01/11 06:00
CRDT- 2022/11/26 18:15
PHST- 2022/08/11 00:00 [received]
PHST- 2022/10/24 00:00 [revised]
PHST- 2022/11/16 00:00 [accepted]
PHST- 2022/11/27 06:00 [pubmed]
PHST- 2023/01/11 06:00 [medline]
PHST- 2022/11/26 18:15 [entrez]
AID - S1052-3057(22)00593-6 [pii]
AID - 10.1016/j.jstrokecerebrovasdis.2022.106901 [doi]
PST - ppublish
SO  - J Stroke Cerebrovasc Dis. 2023 Jan;32(1):106901. doi: 
      10.1016/j.jstrokecerebrovasdis.2022.106901. Epub 2022 Nov 23.