PMID- 36435196
OWN - NLM
STAT- MEDLINE
DCOM- 20230130
LR  - 20230919
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 299
IP  - 1
DP  - 2023 Jan
TI  - Seawater fish use an electrogenic boric acid transporter, Slc4a11A, for boric 
      acid excretion by the kidney.
PG  - 102740
LID - S0021-9258(22)01183-8 [pii]
LID - 10.1016/j.jbc.2022.102740 [doi]
LID - 102740
AB  - Boric acid is a vital micronutrient in animals; however, excess amounts are toxic 
      to them. Little is known about whole-body boric acid homeostasis in animals. 
      Seawater (SW) contains 0.4 mM boric acid, and since marine fish drink SW, their 
      urinary system was used here as a model of the boric acid excretion system. We 
      determined that the bladder urine of a euryhaline pufferfish (river pufferfish, 
      Takifugu obscurus) acclimated to fresh water and SW contained 0.020 and 19 mM of 
      boric acid, respectively (a 950-fold difference), indicating the presence of a 
      powerful excretory renal system for boric acid. Slc4a11 is a potential animal 
      homolog of the plant boron transporter BOR1; however, mammalian Slc4a11 mediates 
      H(+) (OH(-)) conductance but does not transport boric acid. We found that renal 
      expression of the pufferfish paralog of Slc4a11, Slc4a11A, was markedly induced 
      after transfer from fresh water to SW, and Slc4a11A was localized to the apical 
      membrane of kidney tubules. When pufferfish Slc4a11A was expressed in Xenopus 
      oocytes, exposure to media containing boric acid and a voltage clamp elicited 
      whole-cell outward currents, a marked increase in pH(i), and increased boron 
      content. In addition, the activity of Slc4a11A was independent of extracellular 
      Na(+). These results indicate that pufferfish Slc4a11A is an electrogenic boric 
      acid transporter that functions as a B(OH)(4)(-) uniporter, B(OH)(3)-OH(-) 
      cotransporter, or B(OH)(3)/H(+) exchanger. These observations suggest that 
      Slc4a11A is involved in the kidney tubular secretion of boric acid in SW fish, 
      probably induced by the negative membrane potential and low pH of urine.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Kato, Akira
AU  - Kato A
AD  - School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, 
      Japan; Graduate School of Bioscience and Biotechnology, Tokyo Institute of 
      Technology, Yokohama, Japan; Center for Biological Resources and Informatics, 
      Tokyo Institute of Technology, Yokohama, Japan; Department of Physiology and 
      Biomedical Engineering, Mayo Clinic College of Medicine and Science, Rochester, 
      Minnesota, USA. Electronic address: akirkato@bio.titech.ac.jp.
FAU - Kimura, Yuuri
AU  - Kimura Y
AD  - Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 
      Yokohama, Japan.
FAU - Kurita, Yukihiro
AU  - Kurita Y
AD  - Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 
      Yokohama, Japan.
FAU - Chang, Min-Hwang
AU  - Chang MH
AD  - Department of Physiology and Biomedical Engineering, Mayo Clinic College of 
      Medicine and Science, Rochester, Minnesota, USA.
FAU - Kasai, Koji
AU  - Kasai K
AD  - Graduate School of Agricultural and Life Sciences, The University of Tokyo, 
      Tokyo, Japan.
FAU - Fujiwara, Toru
AU  - Fujiwara T
AD  - Graduate School of Agricultural and Life Sciences, The University of Tokyo, 
      Tokyo, Japan.
FAU - Hirata, Taku
AU  - Hirata T
AD  - Department of Physiology and Biomedical Engineering, Mayo Clinic College of 
      Medicine and Science, Rochester, Minnesota, USA.
FAU - Doi, Hiroyuki
AU  - Doi H
AD  - Nifrel, Osaka Aquarium Kaiyukan Co, Ltd, Osaka, Japan.
FAU - Hirose, Shigehisa
AU  - Hirose S
AD  - Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 
      Yokohama, Japan.
FAU - Romero, Michael F
AU  - Romero MF
AD  - Department of Physiology and Biomedical Engineering, Mayo Clinic College of 
      Medicine and Science, Rochester, Minnesota, USA; Nephrology & Hypertension, Mayo 
      Clinic College of Medicine and Science, Rochester, Minnesota, USA; O'Brien 
      Urology Research Center, Mayo Clinic College of Medicine and Science, Rochester, 
      Minnesota, USA. Electronic address: romero.michael@mayo.edu.
LA  - eng
GR  - P50 DK083007/DK/NIDDK NIH HHS/United States
GR  - R01 DK092408/DK/NIDDK NIH HHS/United States
GR  - R01 EY017732/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20221123
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - R57ZHV85D4 (boric acid)
RN  - N9E3X5056Q (Boron)
RN  - 0 (Membrane Transport Proteins)
SB  - IM
MH  - Animals
MH  - *Boron/metabolism
MH  - *Kidney/metabolism
MH  - *Membrane Transport Proteins/metabolism
MH  - Seawater
MH  - Fishes
MH  - Takifugu
PMC - PMC9803922
OTO - NOTNLM
OT  - Slc4a11
OT  - electrogenic boric acid transporter
OT  - electrophysiology
OT  - epithelial cell
OT  - homeostasis
OT  - kidney
OT  - membrane transport
OT  - seawater acclimation
COIS- Conflict of interest The authors declare that they have no conflicts of interest 
      with the contents of this article.
EDAT- 2022/11/27 06:00
MHDA- 2023/01/28 06:00
PMCR- 2022/11/23
CRDT- 2022/11/26 19:22
PHST- 2021/11/15 00:00 [received]
PHST- 2022/11/20 00:00 [revised]
PHST- 2022/11/21 00:00 [accepted]
PHST- 2022/11/27 06:00 [pubmed]
PHST- 2023/01/28 06:00 [medline]
PHST- 2022/11/26 19:22 [entrez]
PHST- 2022/11/23 00:00 [pmc-release]
AID - S0021-9258(22)01183-8 [pii]
AID - 102740 [pii]
AID - 10.1016/j.jbc.2022.102740 [doi]
PST - ppublish
SO  - J Biol Chem. 2023 Jan;299(1):102740. doi: 10.1016/j.jbc.2022.102740. Epub 2022 
      Nov 23.