PMID- 36435808 OWN - NLM STAT- MEDLINE DCOM- 20221129 LR - 20221129 IS - 1297-9716 (Electronic) IS - 0928-4249 (Print) IS - 0928-4249 (Linking) VI - 53 IP - 1 DP - 2022 Nov 26 TI - Anti-inflammatory effects of the prostaglandin D(2)/prostaglandin DP1 receptor and lipocalin-type prostaglandin D(2) synthase/prostaglandin D(2) pathways in bacteria-induced bovine endometrial tissue. PG - 98 LID - 10.1186/s13567-022-01100-6 [doi] LID - 98 AB - Dairy cows often develop different degrees of endometritis after calving and this is attributed to pathogenic bacterial infections such as by Escherichia coli and Staphylococcus aureus. Infection of the bovine endometrium causes tissue damage and increases the expression of prostaglandin D(2) (PGD(2)), which exerts anti-inflammatory effects on lung inflammation. However, the roles of PGD(2) and its DP1 receptor in endometritis in cows remain unclear. Here, we examined the anti-inflammatory roles of the lipocalin-type prostaglandin D(2) synthase (L-PGDS)/PGD(2) and DP1 receptor regulatory pathways in bovine endometritis. We evaluated the regulatory effects of PGD(2) on inflammation and tissue damage in E. coli- and S. aureus-infected bovine endometrial cells cultured in vitro. We found that the secretion of pro-inflammatory cytokines interleukin (IL)-6, IL-1beta, and tumour necrosis factor (TNF)-alpha as well as expression of matrix metalloproteinase (MMP)-2, platelet-activating factor receptor (PAFR), and high mobility group box (HMGB)-1 were suppressed after DP1 receptor agonist treatment. In contrast, IL-6, IL-1beta, and TNF-alpha release and MMP-2, PAFR, and HMGB-1 expression levels were increased after treatment of bovine endometrial tissue with DP1 receptor antagonists. DP1-induced anti-inflammatory effects were dependent on cellular signal transduction. The L-PGDS/PGD(2) pathway and DP1 receptor induced anti-inflammatory effects in bovine endometrium infected with S. aureus and E. coli by inhibiting the mitogen-activated protein kinase and nuclear factor-kappaB signalling pathways, thereby reducing tissue damage. Overall, our findings provide important insights into the pathophysiological roles of PGD(2) in bovine endometritis and establish a theoretical basis for applying prostaglandins or non-steroidal anti-inflammatory drugs for treating endometrial inflammatory infertility in bovines. CI - (c) 2022. The Author(s). FAU - Wu, Jindi AU - Wu J AD - College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China. AD - Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 010018, China. AD - Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Hohhot, 010018, China. FAU - Bai, Fan AU - Bai F AD - Veterinary Research Institute, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, 010031, China. FAU - Mao, Wei AU - Mao W AD - Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 010018, China. AD - Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Hohhot, 010018, China. FAU - Liu, Bo AU - Liu B AD - Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 010018, China. AD - Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Hohhot, 010018, China. FAU - Yang, Xiaolin AU - Yang X AD - Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 010018, China. AD - Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Hohhot, 010018, China. FAU - Zhang, Jing AU - Zhang J AD - Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 010018, China. AD - Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Hohhot, 010018, China. FAU - Li, Tingting AU - Li T AD - Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 010018, China. AD - Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Hohhot, 010018, China. FAU - Borjigin, Gerelt AU - Borjigin G AD - College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China. bor_gerelt07@imau.edu.cn. FAU - Cao, Jinshan AU - Cao J AUID- ORCID: 0000-0001-8751-7667 AD - Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 010018, China. jinshancao@imau.edu.cn. AD - Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Hohhot, 010018, China. jinshancao@imau.edu.cn. LA - eng GR - 31672603/National Natural Science Foundation of China/ GR - 31860720/National Natural Science Foundation of China/ GR - 31860722/National Natural Science Foundation of China/ PT - Journal Article DEP - 20221126 PL - England TA - Vet Res JT - Veterinary research JID - 9309551 RN - 0 (Lipocalins) RN - 0 (Prostaglandins) SB - IM MH - Female MH - Cattle MH - Animals MH - *Endometritis/veterinary MH - Escherichia coli/metabolism MH - Staphylococcus aureus/metabolism MH - Lipocalins/genetics/metabolism MH - Prostaglandins MH - *Cattle Diseases/drug therapy/metabolism PMC - PMC9701374 OTO - NOTNLM OT - DP1 receptor OT - Escherichia coli OT - L-PGDS/PGD2 pathway OT - Staphylococcus aureus OT - anti-inflammatory effects OT - bovine endometrial tissue COIS- The authors declare that they have no competing interests. EDAT- 2022/11/27 06:00 MHDA- 2022/11/30 06:00 PMCR- 2022/11/26 CRDT- 2022/11/26 23:19 PHST- 2021/10/07 00:00 [received] PHST- 2022/07/27 00:00 [accepted] PHST- 2022/11/26 23:19 [entrez] PHST- 2022/11/27 06:00 [pubmed] PHST- 2022/11/30 06:00 [medline] PHST- 2022/11/26 00:00 [pmc-release] AID - 10.1186/s13567-022-01100-6 [pii] AID - 1100 [pii] AID - 10.1186/s13567-022-01100-6 [doi] PST - epublish SO - Vet Res. 2022 Nov 26;53(1):98. doi: 10.1186/s13567-022-01100-6.