PMID- 36436910
OWN - NLM
STAT- MEDLINE
DCOM- 20230519
LR  - 20230525
IS  - 1881-7122 (Electronic)
IS  - 1341-1357 (Print)
IS  - 0007-5124 (Linking)
VI  - 72
IP  - 2
DP  - 2023 May 17
TI  - Impaired healing of cutaneous wound in a Trpv1 deficient mouse.
PG  - 224-232
LID - 10.1538/expanim.22-0124 [doi]
AB  - Transient Receptor Potential (TRP) ion channels mediate the influx of cations 
      into cells responding to chemical or physical stimuli. TRP vanilloid 1 (TRPV1) 
      regulates cutaneous functions. Its function in cutaneous wound healing, however, 
      has not been clarified. The current study elucidated the role of TRPV1 in 
      cutaneous wound healing of dorsal circular excisional injury using Trpv1-null 
      (KO) and wild type (WT) male/female C57BL/6 mice. Macroscopic observation showed 
      that the remaining cutaneous lesion was significantly larger in KO than that of 
      WT at postoperative days (POD) 7 and 10. Histological analysis showed 
      significantly delayed re-epithelialization in KO at POD7. The number of 
      macrophages in KO and WT similarly returned to the reduced state from POD4 to 
      POD7. Whereas, the number of neutrophils in KO did not significantly return to 
      the reduced state, in contrast to WT. Of note, The H3Cit-labeled NETs (Neutrophil 
      Extracellular Traps) formation of KO was prominently increased both in POD4 and 
      7. The current results suggest that the loss of TRPV1 induces prolonged 
      neutrophilic inflammation and NETs formation, retarding murine cutaneous wound 
      healing in vivo. This study provides a possible link with TRPV1 and neutrophilic 
      regulation in cutaneous wound healing.
FAU - Ueno, Kazuki
AU  - Ueno K
AD  - Department of Plastic and Reconstructive Surgery, Wakayama Medical University, 
      811-1 Kimiidera, Wakayama 641-8509, Japan.
FAU - Saika, Shizuya
AU  - Saika S
AD  - Department of Ophthalmology, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama 641-8509, Japan.
FAU - Okada, Yuka
AU  - Okada Y
AD  - Department of Ophthalmology, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama 641-8509, Japan.
FAU - Iwanishi, Hiroki
AU  - Iwanishi H
AD  - Department of Ophthalmology, Wakayama Medical University, 811-1 Kimiidera, 
      Wakayama 641-8509, Japan.
FAU - Suzuki, Kentaro
AU  - Suzuki K
AD  - Department of Developmental Genetics, Institute of Advanced Medicine, Wakayama 
      Medical University, 811-1 Kimiidera, Wakayama 641-8509, Japan.
AD  - Faculty of Life and Environmental Sciences, University of Yamanashi, 4-4-37 
      Takeda, Kofu, Yamanashi 400-8510, Japan.
FAU - Yamada, Gen
AU  - Yamada G
AD  - Department of Plastic and Reconstructive Surgery, Wakayama Medical University, 
      811-1 Kimiidera, Wakayama 641-8509, Japan.
AD  - Department of Developmental Genetics, Institute of Advanced Medicine, Wakayama 
      Medical University, 811-1 Kimiidera, Wakayama 641-8509, Japan.
FAU - Asamura, Shinichi
AU  - Asamura S
AD  - Department of Plastic and Reconstructive Surgery, Wakayama Medical University, 
      811-1 Kimiidera, Wakayama 641-8509, Japan.
LA  - eng
PT  - Journal Article
DEP - 20221125
PL  - Japan
TA  - Exp Anim
JT  - Experimental animals
JID - 9604830
RN  - 0 (TRPV1 protein, mouse)
RN  - 0 (TRPV Cation Channels)
SB  - IM
MH  - Mice
MH  - Female
MH  - Male
MH  - Animals
MH  - Mice, Knockout
MH  - Mice, Inbred C57BL
MH  - *Wound Healing/genetics
MH  - *TRPV Cation Channels/genetics
PMC - PMC10202716
OTO - NOTNLM
OT  - Neutrophil Extracellular Traps (NETs)
OT  - knockout mouse
OT  - neutrophil
OT  - transient receptor potential vanilloid subtype 1 (TRPV1)
OT  - wound healing
COIS- The authors declare no conflict of interest.
EDAT- 2022/11/28 06:00
MHDA- 2023/05/19 06:42
PMCR- 2023/01/01
CRDT- 2022/11/27 21:07
PHST- 2023/05/19 06:42 [medline]
PHST- 2022/11/28 06:00 [pubmed]
PHST- 2022/11/27 21:07 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 22-0124 [pii]
AID - 10.1538/expanim.22-0124 [doi]
PST - ppublish
SO  - Exp Anim. 2023 May 17;72(2):224-232. doi: 10.1538/expanim.22-0124. Epub 2022 Nov 
      25.