PMID- 36437414
OWN - NLM
STAT- MEDLINE
DCOM- 20230218
LR  - 20240210
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 482
IP  - 2
DP  - 2023 Feb
TI  - Differential HLA class I subunit (A, B, C heavy chain and beta(2)-microglobulin) 
      expression levels in normal tissues.
PG  - 359-368
LID - 10.1007/s00428-022-03459-5 [doi]
AB  - Human leukocyte antigen (HLA) class I subunit expression level in primary and 
      metastatic lesions has been characterized in many cancer types utilizing 
      formalin-fixed and paraffin-embedded (FFPE) tissue sections as substrates in 
      immunohistochemical reactions. The evaluation of the results of these studies has 
      been hampered by the scant information about HLA class I subunit expression level 
      in normal tissues. To address this unmet need, we have analyzed the HLA class I 
      subunit expression level in FFPE sections of normal tissues.Two tissue microarray 
      (TMA) blocks were constructed from archived FFPE tissue samples of a wide number 
      of human normal tissues. The expression level of HLA-A, HLA-B, HLA-C heavy chains 
      and beta2-microglobulin (beta2-M) was evaluated by IHC staining, with mAb HC-A2, mAb 
      HC-10, and mAb NAMB1, respectively. The staining was scored according to its 
      intensity.According to their staining patterns with the three mAbs tested, normal 
      tissues can be divided into four groups: (i) tissues displaying moderate/strong 
      staining patterns, (ii) tissues displaying barely detectable staining patterns, 
      (iii) tissues displaying differential staining patterns, and (iv) tissues with no 
      detectable staining. The ubiquitous expression pattern for HLA-A, B, C heavy 
      chain and beta2-M was found only at the endothelial level; the stroma was negative 
      except for fibroblasts in all the tissues analyzed. Our data suggest that, 
      contrary to the general postulate, HLA class I subunit expression is not 
      detectable in all nucleated cells. This information provides a useful background 
      to evaluate changes in HLA class I subunit expression associated with the 
      malignant transformation of cells.
CI  - (c) 2022. The Author(s).
FAU - Ugolini, Filippo
AU  - Ugolini F
AD  - Department of Health Sciences, Section of Pathological Anatomy, University of 
      Florence, Viale Pieraccini 6, 50139, Florence, Italy.
FAU - Szumera-Cieckiewicz, Anna
AU  - Szumera-Cieckiewicz A
AUID- ORCID: 0000-0001-5028-3422
AD  - Department of Pathology, Maria Sklodowska-Curie National Research Institute of 
      Oncology, Warsaw, Poland.
AD  - Diagnostic Hematology Department, Institute of Hematology and Transfusion 
      Medicine, Warsaw, Poland.
FAU - Baroni, Gianna
AU  - Baroni G
AUID- ORCID: 0000-0003-2247-6774
AD  - Department of Health Sciences, Section of Pathological Anatomy, University of 
      Florence, Viale Pieraccini 6, 50139, Florence, Italy.
FAU - Nesi, Gabriella
AU  - Nesi G
AUID- ORCID: 0000-0002-2614-944X
AD  - Department of Health Sciences, Section of Pathological Anatomy, University of 
      Florence, Viale Pieraccini 6, 50139, Florence, Italy.
FAU - Mandala, Mario
AU  - Mandala M
AUID- ORCID: 0000-0001-8846-8959
AD  - Unit of Medical Oncology, University of Perugia, Perugia, Italy.
FAU - Ferrone, Soldano
AU  - Ferrone S
AUID- ORCID: 0000-0003-2900-8834
AD  - Department of Surgery, Massachusetts General Hospital, Harvard Medical School, 
      Boston, MA, USA.
FAU - Massi, Daniela
AU  - Massi D
AUID- ORCID: 0000-0002-5688-5923
AD  - Department of Health Sciences, Section of Pathological Anatomy, University of 
      Florence, Viale Pieraccini 6, 50139, Florence, Italy. daniela.massi@unifi.it.
LA  - eng
PT  - Journal Article
DEP - 20221127
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-A Antigens)
RN  - 0 (beta 2-Microglobulin)
SB  - IM
EIN - Virchows Arch. 2024 Feb 5;:. PMID: 38315233
MH  - Humans
MH  - *Antibodies, Monoclonal
MH  - *Histocompatibility Antigens Class I
MH  - HLA Antigens
MH  - HLA-A Antigens
MH  - beta 2-Microglobulin
PMC - PMC9931818
OTO - NOTNLM
OT  - HLA class I
OT  - HLA-ABC
OT  - Healthy tissues
OT  - TMA
OT  - beta2-microglobulin
COIS- The authors declare no competing interests.
EDAT- 2022/11/28 06:00
MHDA- 2023/02/18 06:00
PMCR- 2022/11/27
CRDT- 2022/11/27 23:28
PHST- 2022/10/05 00:00 [received]
PHST- 2022/11/16 00:00 [accepted]
PHST- 2022/11/14 00:00 [revised]
PHST- 2022/11/28 06:00 [pubmed]
PHST- 2023/02/18 06:00 [medline]
PHST- 2022/11/27 23:28 [entrez]
PHST- 2022/11/27 00:00 [pmc-release]
AID - 10.1007/s00428-022-03459-5 [pii]
AID - 3459 [pii]
AID - 10.1007/s00428-022-03459-5 [doi]
PST - ppublish
SO  - Virchows Arch. 2023 Feb;482(2):359-368. doi: 10.1007/s00428-022-03459-5. Epub 
      2022 Nov 27.