PMID- 36440854
OWN - NLM
STAT- MEDLINE
DCOM- 20230202
LR  - 20230216
IS  - 1522-1563 (Electronic)
IS  - 0363-6143 (Linking)
VI  - 324
IP  - 2
DP  - 2023 Feb 1
TI  - LncRNA NEAT1 accelerates renal fibrosis progression via targeting miR-31 and 
      modulating RhoA/ROCK signal pathway.
PG  - C292-C306
LID - 10.1152/ajpcell.00382.2021 [doi]
AB  - Renal fibrosis is the final pathway for chronic kidney disease to end-stage renal 
      failure. Noncoding RNAs have been reported to play a crucial role in renal 
      fibrosis. Here, the effects of long noncoding RNA (lncRNA) nuclear-enriched 
      abundant transcript 1 (NEAT1) and miR-31 on renal fibrosis and their regulatory 
      mechanism were evaluated. RT-qPCR was used to assess NEAT1, miR-31, and RhoA 
      levels. Western blot was performed to analyze the expression of fibrosis markers, 
      RhoA, rho-related kinase (ROCK1), and connective tissue growth factor (CTGF). RNA 
      immunoprecipitation (RIP), fluorescence in situ hybridization (FISH), and 
      luciferase reporter assays verified the interaction between miR-31 and NEAT1 or 
      RhoA. Renal fibrosis and injury were observed by Masson and hematoxylin and eosin 
      (H&E) staining. The expression level of inflammatory cytokines was detected by 
      ELISA. Immunohistochemistry (IHC) was performed to examine the expression levels 
      of alpha-smooth muscle actin (alpha-SMA) and RhoA in renal tissues. We showed that NEAT1 
      was highly expressed, whereas miR-31 was decreased in renal fibrosis. NEAT1 was 
      found to directly bind miR-31 to positively regulate RhoA expression. 
      Furthermore, NEAT1 silencing inhibited renal fibrosis and inflammation and 
      suppressed the RhoA/ROCK1 signaling pathway. However, knockdown of miR-31 could 
      reverse these effects. NEAT1 silencing or overexpression of miR-31 alleviated 
      renal fibrosis in vivo. In conclusion, NEAT1 accelerates renal fibrosis 
      progression via negative regulation of miR-31 and the activation of RhoA/ROCK1 
      pathway, thereby upregulating the expression level of CTGF, providing a 
      theoretical basis for treatment and prognostic evaluation of renal fibrosis.
FAU - Chen, Yan
AU  - Chen Y
AD  - Department of Nephrology, The Second Affiliated Hospital of Nanchang University, 
      Nanchang, China.
FAU - Huang, Chong
AU  - Huang C
AD  - Department of Nephrology, The Second Affiliated Hospital of Nanchang University, 
      Nanchang, China.
FAU - Duan, Zhi-Bin
AU  - Duan ZB
AD  - Department of Nephrology, The Second Affiliated Hospital of Nanchang University, 
      Nanchang, China.
FAU - Chen, Yan-Xia
AU  - Chen YX
AD  - Department of Nephrology, The Second Affiliated Hospital of Nanchang University, 
      Nanchang, China.
FAU - Xu, Cheng-Yun
AU  - Xu CY
AUID- ORCID: 0000-0003-3747-3728
AD  - Department of Nephrology, The Second Affiliated Hospital of Nanchang University, 
      Nanchang, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221128
PL  - United States
TA  - Am J Physiol Cell Physiol
JT  - American journal of physiology. Cell physiology
JID - 100901225
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 124671-05-2 (RHOA protein, human)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
RN  - EC 2.7.11.1 (ROCK1 protein, human)
RN  - EC 2.7.11.1 (rho-Associated Kinases)
RN  - 0 (MIRN31 microRNA, human)
SB  - IM
MH  - Humans
MH  - *MicroRNAs/genetics/metabolism
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Fibrosis
MH  - *Kidney Diseases
MH  - Signal Transduction
MH  - Apoptosis
MH  - rhoA GTP-Binding Protein/genetics/metabolism
MH  - rho-Associated Kinases/genetics/metabolism
OTO - NOTNLM
OT  - RhoA/ROCK1 signal pathway
OT  - lncRNA NEAT1
OT  - miR-31
OT  - renal fibrosis
EDAT- 2022/11/29 06:00
MHDA- 2023/02/03 06:00
CRDT- 2022/11/28 08:23
PHST- 2022/11/29 06:00 [pubmed]
PHST- 2023/02/03 06:00 [medline]
PHST- 2022/11/28 08:23 [entrez]
AID - 10.1152/ajpcell.00382.2021 [doi]
PST - ppublish
SO  - Am J Physiol Cell Physiol. 2023 Feb 1;324(2):C292-C306. doi: 
      10.1152/ajpcell.00382.2021. Epub 2022 Nov 28.