PMID- 36442386
OWN - NLM
STAT- MEDLINE
DCOM- 20230124
LR  - 20230201
IS  - 1879-0070 (Electronic)
IS  - 0732-8893 (Linking)
VI  - 105
IP  - 2
DP  - 2023 Feb
TI  - BIOFIRE(R) Blood Culture IDentification 2 (BCID2) panel for early adaptation of 
      antimicrobial therapy in adult patients with bloodstream infections: a real-life 
      experience.
PG  - 115858
LID - S0732-8893(22)00222-X [pii]
LID - 10.1016/j.diagmicrobio.2022.115858 [doi]
AB  - Our objective was to assess the effectiveness of a multiplex PCR panel for blood 
      culture identification (BCID2) on the implementation of appropriate antimicrobial 
      therapy. We conducted a monocentric pre/post study comparing the time to result 
      from direct microscopic examination (DE) to bacterial identification (BI) in 
      positive blood cultures between 2 different periods: P1 without BCID2 and P2 with 
      BCID2. Appropriate treatments prescribed before DE and after DE / BCID2 and after 
      BI / BCID2 were compared using direct proportion comparison and survival 
      analysis. For mono-microbial bloodstream infections, the proportion of 
      appropriate antimicrobial treatment after DE was 50% in P1 vs. 87.5% after BCID2 
      in P2 (P < 0.001) for Gram-negative bacteria and 33.0% in P1 vs. 64.4% in P2 (P < 
      0.01) for Gram-positive bacteria. A significant difference (P = 0.04) was 
      recorded with survival curves for Gram positive bacteria. BCID2 seems effective 
      in reducing the time for prescribing appropriate antimicrobials.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Donnars, Anne
AU  - Donnars A
AD  - Laboratoire de Bacteriologie, Departement de Biologie des Agents Infectieux, CHU 
      Angers, Angers, France.
FAU - Mahieu, Rafael
AU  - Mahieu R
AD  - Service des Maladies Infectieuses et Tropicales, CHU Angers, Angers, France; Univ 
      Angers, Nantes Universite, CHU Angers, Inserm, CNRS, INCIT, Angers, France.
FAU - Declerck, Charles
AU  - Declerck C
AD  - Service des Maladies Infectieuses et Tropicales, CHU Angers, Angers, France.
FAU - Chenouard, Rachel
AU  - Chenouard R
AD  - Laboratoire de Bacteriologie, Departement de Biologie des Agents Infectieux, CHU 
      Angers, Angers, France.
FAU - Lemarie, Carole
AU  - Lemarie C
AD  - Laboratoire de Bacteriologie, Departement de Biologie des Agents Infectieux, CHU 
      Angers, Angers, France.
FAU - Pailhories, Helene
AU  - Pailhories H
AD  - Laboratoire de Bacteriologie, Departement de Biologie des Agents Infectieux, CHU 
      Angers, Angers, France.
FAU - Requin, Jim
AU  - Requin J
AD  - Service des Maladies Infectieuses et Tropicales, CHU Angers, Angers, France.
FAU - Kempf, Marie
AU  - Kempf M
AD  - Laboratoire de Bacteriologie, Departement de Biologie des Agents Infectieux, CHU 
      Angers, Angers, France; Univ Angers, Nantes Universite, CHU Angers, Inserm, CNRS, 
      INCIT, Angers, France.
FAU - Eveillard, Matthieu
AU  - Eveillard M
AD  - Laboratoire de Bacteriologie, Departement de Biologie des Agents Infectieux, CHU 
      Angers, Angers, France; Univ Angers, Nantes Universite, CHU Angers, Inserm, CNRS, 
      INCIT, Angers, France. Electronic address: maeveillard@chu-angers.fr.
LA  - eng
PT  - Journal Article
DEP - 20221111
PL  - United States
TA  - Diagn Microbiol Infect Dis
JT  - Diagnostic microbiology and infectious disease
JID - 8305899
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Humans
MH  - Adult
MH  - Blood Culture
MH  - *Anti-Infective Agents/therapeutic use
MH  - Microscopy
MH  - Multiplex Polymerase Chain Reaction
MH  - *Sepsis
MH  - *Bacteremia/diagnosis/drug therapy
MH  - Anti-Bacterial Agents/therapeutic use
OTO - NOTNLM
OT  - Antimicrobial therapy
OT  - Bacterial identification
OT  - Blood cultures
OT  - Multiplex PCR
OT  - Rapid diagnostics
EDAT- 2022/11/29 06:00
MHDA- 2023/01/25 06:00
CRDT- 2022/11/28 18:23
PHST- 2022/08/21 00:00 [received]
PHST- 2022/10/24 00:00 [revised]
PHST- 2022/11/05 00:00 [accepted]
PHST- 2022/11/29 06:00 [pubmed]
PHST- 2023/01/25 06:00 [medline]
PHST- 2022/11/28 18:23 [entrez]
AID - S0732-8893(22)00222-X [pii]
AID - 10.1016/j.diagmicrobio.2022.115858 [doi]
PST - ppublish
SO  - Diagn Microbiol Infect Dis. 2023 Feb;105(2):115858. doi: 
      10.1016/j.diagmicrobio.2022.115858. Epub 2022 Nov 11.