PMID- 36447801
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221202
IS  - 1650-3414 (Electronic)
IS  - 1650-3414 (Linking)
VI  - 33
IP  - 3
DP  - 2022 Oct
TI  - Establishment of Population Specific Reference Intervals in Healthy Pakistani 
      Adults for 21 Routine and Special Haematology Analytes.
PG  - 220-232
AB  - BACKGROUND: The reference interval (RI) is an interval between two limits derived 
      from distribution of the results obtained from a sample of the reference 
      population. These population based RIs are of paramount significance for the 
      accurate clinical understanding of the patient's health status. Haematological 
      RIs are heavily influenced by a variety of geographical and environmental 
      factors. Therefore, accrediting bodies also mandate that each laboratory should 
      establish its own RIs in its own population. METHODS: This cross-sectional study 
      was conducted at the Department of Pathology and Laboratory Medicine, the Aga 
      Khan University Hospital, Pakistan.Twenty-one routine and special quantitative 
      analytes were measured in adults aged 18-60 years who passed the initial health 
      screening questionnaire. All samples were handled strictly following standard 
      operating procedures. Microsoft Excel and EP Evaluator software were used for 
      statistical analysis. Nonparametric CLSI EP28-A3C method was used to establish 
      upper and lower confidence limits at 90% significance. RESULTS: A total of 323 
      participants passed the questionnaire and were short-listed for blood collection. 
      There were 147 males and 176 females. Reference intervals were established in 297 
      participants after exclusion of 26 outliers with grossly abnormal test results. 
      Analytes included: 8 red, and 12 white blood cell parameters, platelet count, 
      immature platelet fraction, erythrocyte sedimentation levels, haemoglobin A and 
      A2 levels and glucose-6-phosphatase dehydrogenase levels. CONCLUSION: Routine and 
      special haematology RIs established in this study reflect significant differences 
      from RIs in Caucasian population. For meaningful interpretation of test results, 
      each haematology laboratory should establish or verify RIs in the population it 
      serves.
CI  - Copyright (c) 2022 International Federation of Clinical Chemistry and Laboratory 
      Medicine (IFCC). All rights reserved.
FAU - Shaikh, Muhammad Shariq
AU  - Shaikh MS
AD  - Department of Pathology and Laboratory Medicine, Aga Khan University Hospital, 
      Karachi, Pakistan.
FAU - Ahmed, Sibtain
AU  - Ahmed S
AD  - Department of Pathology and Laboratory Medicine, Aga Khan University Hospital, 
      Karachi, Pakistan.
FAU - Khalid, Attika
AU  - Khalid A
AD  - Fauji Foundation Hospital, Islamabad, Pakistan.
FAU - Hayat, Muhammad Hasan
AU  - Hayat MH
AD  - Department of Pathology and Laboratory Medicine, Aga Khan University Hospital, 
      Karachi, Pakistan.
FAU - Shaikh, Mohammad Usman
AU  - Shaikh MU
AD  - Department of Pathology and Laboratory Medicine, Aga Khan University Hospital, 
      Karachi, Pakistan.
FAU - Ali, Natasha
AU  - Ali N
AD  - Department of Pathology and Laboratory Medicine, Aga Khan University Hospital, 
      Karachi, Pakistan.
LA  - eng
PT  - Journal Article
DEP - 20221028
PL  - Italy
TA  - EJIFCC
JT  - EJIFCC
JID - 101092742
PMC - PMC9644092
OTO - NOTNLM
OT  - Pakistan
OT  - hematology
OT  - reference interval
COIS- The authors are grateful to laboratory staff who helped with handling of 
      specimens and analysis of data. Competing interests The authors declare that they 
      have no competing interests.
EDAT- 2022/12/01 06:00
MHDA- 2022/12/01 06:01
PMCR- 2022/10/28
CRDT- 2022/11/30 01:59
PHST- 2022/11/30 01:59 [entrez]
PHST- 2022/12/01 06:00 [pubmed]
PHST- 2022/12/01 06:01 [medline]
PHST- 2022/10/28 00:00 [pmc-release]
AID - ejifcc-33-220 [pii]
PST - epublish
SO  - EJIFCC. 2022 Oct 28;33(3):220-232. eCollection 2022 Oct.