PMID- 36448431
OWN - NLM
STAT- MEDLINE
DCOM- 20221201
LR  - 20221214
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 23
IP  - 6
DP  - 2022 Nov
TI  - Antimicrobial resistance studies in staphylococci and streptococci isolated from 
      cows with mastitis in Argentina.
PG  - e12
LID - 10.4142/jvs.21062 [doi]
LID - e12
AB  - BACKGROUND: Staphylococcus aureus and Streptococcus agalactiae are the main cause 
      of clinical mastitis in dairy cattle in Argentina, whereas coagulase-negative 
      staphylococci (CNS) and environmental streptococci are the main cause of 
      subclinical mastitis. Bacteria isolated from infected animals show increasing 
      antimicrobial resistance. OBJECTIVES: This study aims to determine the 
      antimicrobial resistance of staphylococci and streptococci isolated from milk 
      with mastitis, and to genotypically characterize the methicillin-resistant (MR) 
      staphylococci. METHODS: Isolation was performed on blood agar and identification 
      was based on biochemical reactions. Antimicrobial susceptibility was according to 
      the Clinical and Laboratory Standards Institute guidelines. The antimicrobial 
      resistance genes, SCCmec type and spa type were detected by the polymerase chain 
      reaction method. RESULTS: We isolated a total of 185 staphylococci and 28 
      streptococci from 148 milk samples. Among the staphylococcal isolates, 154 were 
      identified as CNS and 31 as S. aureus. Among the 154 CNS, 24.6% (n = 38) were 
      resistant to penicillin, 14.9% (n = 23) to erythromycin, 17.5% (n = 27) to 
      clindamycin, 6.5% (n = 10) to cefoxitin and oxacillin. Among the S. aureus 
      isolates, 16.1% (n = 5) were resistant to penicillin, 3.2% (n = 1) to cefoxitin 
      and oxacillin (MRSA). Six MR isolates (5 CNS and 1 MRSA) were positive to the 
      mecA gene, and presented the SCCmec IVa. The MRSA strain presented the sequence 
      type 83 and the spa type 002. Among the 28 streptococcal isolates, 14.3% (n = 4) 
      were resistant to penicillin, 10.7% (n = 3) to erythromycin and 14.3% (n = 4) to 
      clindamycin. CONCLUSIONS: The present findings of this study indicate a 
      development of antimicrobial resistance in main bacteria isolated from cows with 
      mastitis in Argentina.
CI  - (c) 2022 The Korean Society of Veterinary Science.
FAU - Crespi, Elisa
AU  - Crespi E
AUID- ORCID: 0000-0003-0215-2342
AD  - Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Catedra de 
      Microbiologia, Av. Chorroarin 280, Buenos Aires C1427CWO, Argentina.
FAU - Pereyra, Ana M
AU  - Pereyra AM
AUID- ORCID: 0000-0003-0941-232X
AD  - Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Catedra de 
      Microbiologia, Av. Chorroarin 280, Buenos Aires C1427CWO, Argentina.
FAU - Puigdevall, Tomas
AU  - Puigdevall T
AUID- ORCID: 0000-0001-7203-6932
AD  - Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Catedra de 
      Microbiologia, Av. Chorroarin 280, Buenos Aires C1427CWO, Argentina.
FAU - Rumi, Maria V
AU  - Rumi MV
AUID- ORCID: 0000-0002-4102-1890
AD  - Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Catedra de 
      Microbiologia, Av. Chorroarin 280, Buenos Aires C1427CWO, Argentina.
FAU - Testorelli, Maria F
AU  - Testorelli MF
AUID- ORCID: 0000-0002-5177-2268
AD  - Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Catedra de 
      Microbiologia, Av. Chorroarin 280, Buenos Aires C1427CWO, Argentina.
FAU - Caggiano, Nicolas
AU  - Caggiano N
AUID- ORCID: 0000-0002-1175-0152
AD  - Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Catedra de 
      Fisiologia Animal, Av. Chorroarin 280, Buenos Aires C1427CWO, Argentina.
FAU - Gulone, Lucia
AU  - Gulone L
AUID- ORCID: 0000-0001-9898-6565
AD  - Universidad de Buenos Aires, Facultad de Farmacia y Bioquimica, Catedra de 
      Microbiologia, Junin 954, Buenos Aires C1113AAD, Argentina.
FAU - Mollerach, Marta
AU  - Mollerach M
AUID- ORCID: 0000-0002-5735-8471
AD  - Universidad de Buenos Aires, Facultad de Farmacia y Bioquimica, Catedra de 
      Microbiologia, Junin 954, Buenos Aires C1113AAD, Argentina.
FAU - Gentilini, Elida R
AU  - Gentilini ER
AUID- ORCID: 0000-0002-5366-3460
AD  - Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Catedra de 
      Microbiologia, Av. Chorroarin 280, Buenos Aires C1427CWO, Argentina.
FAU - Srednik, Mariela E
AU  - Srednik ME
AUID- ORCID: 0000-0002-7387-1793
AD  - Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Catedra de 
      Microbiologia, Av. Chorroarin 280, Buenos Aires C1427CWO, Argentina. 
      maru_sred@fvet.uba.ar.
LA  - eng
GR  - 20020170100084BA/Secretaria de Ciencia y Tecnica, Universidad de Buenos 
      Aires/Argentina
PT  - Journal Article
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
RN  - 0 (Anti-Bacterial Agents)
RN  - 6OEV9DX57Y (Cefoxitin)
RN  - 3U02EL437C (Clindamycin)
RN  - UH95VD7V76 (Oxacillin)
RN  - 63937KV33D (Erythromycin)
SB  - IM
MH  - Female
MH  - Cattle
MH  - Animals
MH  - Staphylococcus
MH  - Anti-Bacterial Agents/pharmacology
MH  - Staphylococcus aureus
MH  - Cefoxitin
MH  - Clindamycin
MH  - Argentina/epidemiology
MH  - Drug Resistance, Bacterial
MH  - *Mastitis, Bovine/epidemiology
MH  - Streptococcus
MH  - Oxacillin
MH  - Erythromycin
MH  - *Cattle Diseases
PMC - PMC9715389
OTO - NOTNLM
OT  - Bovine mastitis
OT  - antimicrobial resistance
OT  - molecular typing
OT  - staphylococci
OT  - streptococci
COIS- The authors declare no conflicts of interest.
EDAT- 2022/12/01 06:00
MHDA- 2022/12/02 06:00
PMCR- 2022/11/01
CRDT- 2022/11/30 04:53
PHST- 2021/03/13 00:00 [received]
PHST- 2021/07/26 00:00 [revised]
PHST- 2021/09/15 00:00 [accepted]
PHST- 2022/11/30 04:53 [entrez]
PHST- 2022/12/01 06:00 [pubmed]
PHST- 2022/12/02 06:00 [medline]
PHST- 2022/11/01 00:00 [pmc-release]
AID - 23.e12 [pii]
AID - 10.4142/jvs.21062 [doi]
PST - ppublish
SO  - J Vet Sci. 2022 Nov;23(6):e12. doi: 10.4142/jvs.21062.