PMID- 36450504
OWN - NLM
STAT- MEDLINE
DCOM- 20221227
LR  - 20221230
IS  - 1881-784X (Electronic)
IS  - 1881-7831 (Linking)
VI  - 16
IP  - 6
DP  - 2022 Dec 26
TI  - Silkworm arylsulfatase in the midgut content is expressed in the silk gland and 
      fed via smearing on the food from the spinneret.
PG  - 280-285
LID - 10.5582/ddt.2022.01088 [doi]
AB  - We found the activity of arylsulfatase in the midgut contents of the silkworm, 
      Bombyx mori. We identified a 60-kDa protein that comigrates with the activity on 
      a column chromatography following ammonium sulfate precipitation. Based on its 
      partial amino acid sequence, we searched for its coding gene using Basic Local 
      Alignment Search Tool (BLAST) and identified KWMTBOMO05106. Transcriptional data 
      suggest a specific expression of the gene in middle silk glands. The majority 
      (80%) of arylsulfatase activity was found in the silk glands, concurring the 
      specific transcription in the silk gland. Observing the feeding behaviour of the 
      silkworm, we found that silkworms smear a mucus secretes from the spinneret on 
      the food pellet as they feed on. Arylsulfatase activity was also detected in the 
      food pellet bitten by the silkworm as well as in the gut content. Furthermore, 
      arylsulfatase activity was not detected either in the food pellet and in the gut 
      content when silkworms had obstructed the spinneret. These results suggest that 
      arylsulfatase is secreted from the silk glands and may contribute to digestive 
      function.
FAU - Hamamoto, Hiroshi
AU  - Hamamoto H
AD  - Teikyo University Institute of Medical Mycology, Tokyo, Japan.
FAU - Miyashita, Atsushi
AU  - Miyashita A
AD  - Teikyo University Institute of Medical Mycology, Tokyo, Japan.
FAU - Kamura, Koushirou
AU  - Kamura K
AD  - Drug Discoveries by Silkworm Models, Faculty of Pharma-Science, Teikyo 
      University, Tokyo, Japan.
FAU - Horie, Ryo
AU  - Horie R
AD  - Drug Discoveries by Silkworm Models, Faculty of Pharma-Science, Teikyo 
      University, Tokyo, Japan.
FAU - Sekimizu, Kazuhisa
AU  - Sekimizu K
AD  - Drug Discoveries by Silkworm Models, Faculty of Pharma-Science, Teikyo 
      University, Tokyo, Japan.
LA  - eng
PT  - Journal Article
DEP - 20221201
PL  - Japan
TA  - Drug Discov Ther
JT  - Drug discoveries & therapeutics
JID - 101493809
RN  - 0 (Silk)
RN  - EC 3.1.6.1 (Arylsulfatases)
RN  - 0 (Insect Proteins)
SB  - IM
MH  - Animals
MH  - *Bombyx/chemistry
MH  - Silk/genetics/metabolism
MH  - Arylsulfatases/metabolism
MH  - Insect Proteins/genetics/metabolism
OTO - NOTNLM
OT  - arylsulfatase
OT  - enzyme
OT  - secretion
OT  - silkworm
EDAT- 2022/12/01 06:00
MHDA- 2022/12/28 06:00
CRDT- 2022/11/30 21:13
PHST- 2022/12/01 06:00 [pubmed]
PHST- 2022/12/28 06:00 [medline]
PHST- 2022/11/30 21:13 [entrez]
AID - 10.5582/ddt.2022.01088 [doi]
PST - ppublish
SO  - Drug Discov Ther. 2022 Dec 26;16(6):280-285. doi: 10.5582/ddt.2022.01088. Epub 
      2022 Dec 1.