PMID- 36453206
OWN - NLM
STAT- MEDLINE
DCOM- 20230119
LR  - 20230214
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 32
IP  - 1-2
DP  - 2023 Jan
TI  - Monocyte Chemoattractant Protein-1-Supplemented Plasma Enhances Adiponectin and 
      Adipogenesis-Related Gene Expression.
PG  - 32-43
LID - 10.1089/scd.2022.0227 [doi]
AB  - Increasing adipogenesis has been explored to treat metabolic diseases and 
      atherosclerosis through the release of adiponectin. The effects and mechanism of 
      platelet-rich plasma treatment on fat graft survival and adipogenesis have not 
      been clarified. Here, we aimed to study the effects of monocyte chemoattractant 
      protein-1 (MCP-1)-supplemented plasma on adipogenesis-related gene expression and 
      adiponectin levels. Stromal vascular fractions (SVFs) purified from the inguinal 
      adipose tissue of obese and diabetic (Lepr(db/db)) mice were treated with plasma 
      from control (Lepr(+/+)) mice supplemented with 10 or 50 ng of MCP-1. The 
      expression of adiponectin and interleukin-33 (IL-33) mRNA in adipose tissue was 
      increased in Lepr(db/db) mice, whereas control (Lepr(+/+)) plasma reduced 
      expression of IL-33 mRNA as well as peroxisome proliferator-activated receptor 
      gamma (PPARgamma), pJNK, and pNF-kappaB protein, and increased the expression of IL-10 
      mRNA in SVFs of Lepr(db/db) mice. MCP-1-supplemented control plasma increased the 
      expression of adiponectin, CCAAT-enhancer-binding protein alpha (C/EBPalpha), dipeptidyl 
      peptidase 4 (DPP4), IL-33, and PDGFalpha mRNA and the expression of adiponectin 
      protein as well as PPARgamma of SVFs and the expression of PPARgamma mRNA in adipose 
      tissue macrophages (ATMs). Injection of MCP-1-supplemented plasma into adipose 
      tissue of Lepr(db/db) mice increased the expression of IL-33 and Col3a1 mRNA in 
      SVFs and IL-33, FABP4, PDGFalpha, PPARgamma and PPARgamma2 of ATMs, protein expression of 
      adiponectin and PPARgamma of SVFs, and plasma adiponectin levels, as well as DPP4 
      activity. In conclusion, our results demonstrate that control plasma decreases 
      adipogenesis and increases IL-10, and decreases IL-33, pJNK, and pNF-kappaB in 
      adipose tissue. MCP-1-supplemented plasma enhances adipogenesis-related gene 
      expression in SVFs and adiponectin levels, which may be mediated through an 
      increase of IL-33 and PPARgamma. Thus, our findings suggest that MCP-1-supplemented 
      plasma represents a novel therapy to stimulate local adipogenesis and systemic 
      adiponectin levels.
FAU - Tsay, Tzyy-Bin
AU  - Tsay TB
AD  - Department of Surgery, Kaohsiung Armed Forces General Hospital Zuoying Branch, 
      Kaohsiung, Taiwan.
FAU - Chen, Pei-Hsuan
AU  - Chen PH
AD  - Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
FAU - Li, Merton
AU  - Li M
AD  - Department of Biological Sciences, USC Dornsife College of Letters, Arts and 
      Sciences, University of Southern California, Los Angeles, California, USA.
FAU - Tang, Chia-Hua
AU  - Tang CH
AD  - Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
FAU - Chen, Lee-Wei
AU  - Chen LW
AUID- ORCID: 0000-0002-3583-1248
AD  - Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
AD  - Institute of Emergency and Critical Care Medicine, National Yang Ming Chiao Tung 
      University, Taipei, Taiwan.
AD  - Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, 
      Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230102
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Adiponectin)
RN  - 0 (Chemokine CCL2)
RN  - EC 3.4.14.5 (Dipeptidyl Peptidase 4)
RN  - 130068-27-8 (Interleukin-10)
RN  - 0 (Interleukin-33)
RN  - 0 (PPAR gamma)
RN  - 0 (RNA, Messenger)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Adipoq protein, mouse)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Adipogenesis/genetics
MH  - *Adiponectin/genetics/metabolism
MH  - *Chemokine CCL2/genetics/metabolism
MH  - Dipeptidyl Peptidase 4/genetics
MH  - Gene Expression
MH  - Interleukin-10/genetics
MH  - Interleukin-33/genetics
MH  - PPAR gamma/genetics/metabolism
MH  - RNA, Messenger/metabolism
OTO - NOTNLM
OT  - IL-10
OT  - PDGFalpha
OT  - adiponectin
OT  - adipose tissue macrophages
OT  - stromal vascular fractions
EDAT- 2022/12/02 06:00
MHDA- 2023/01/18 06:00
CRDT- 2022/12/01 04:43
PHST- 2022/12/02 06:00 [pubmed]
PHST- 2023/01/18 06:00 [medline]
PHST- 2022/12/01 04:43 [entrez]
AID - 10.1089/scd.2022.0227 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2023 Jan;32(1-2):32-43. doi: 10.1089/scd.2022.0227. Epub 2023 Jan 
      2.