PMID- 36453958
OWN - NLM
STAT- MEDLINE
DCOM- 20221206
LR  - 20221206
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 39
IP  - 12
DP  - 2022 Dec 10
TI  - [Association of gene expression in ovarian granulosa cells with embryonic 
      development potential among patients with polycystic ovary syndrome].
PG  - 1354-1359
LID - 10.3760/cma.j.cn511374-20211109-00891 [doi]
AB  - OBJECTIVE: To assess the association of gene expression with development 
      potential of early embryos derived from patients with polycystic ovary syndrome 
      (PCOS). METHODS: Three pairs of infertile patients with respectively matched age, 
      body mass index, ovarian reserve and treatment with gonadotrophin-releasing 
      hormone (GnRH) antagonists were selected. Patients with fewer embryos were 
      assigned as the case group (n = 3), whilst the remainders were assigned as the 
      control group (n = 3). Ovarian granulosa cells from patients were collected for 
      the extraction of total RNA and subjected to RNA sequencing. The results were 
      subjected to differential gene expression and functional enrichment analyses. 
      RESULTS: Compared with the control group, 76 genes were up-regulated and 110 
      genes were down-regulated in the case group. The level of estradiol (E(2)) was 
      significantly higher in the control group on the trigger day with the injection 
      of human chorionic gonadotrophin (HCG). Compared with the control group, the KRT7 
      gene was most significantly up-regulated, whilst the CCNYL2 gene was most 
      significantly down-regulated in the case group. Gene ontology (GO) entries 
      enrichment has found those associated with chromosome segregation, cell cycle 
      regulation, and fatty acid metabolism to be significantly enriched. The genes 
      participating in the regulation of cell assembly, differentiation, negative 
      regulation of cell cycle, negative regulation of development, extracellular 
      regulated protein kinases (ERK), ERK1 and ERK2 signaling pathways to be 
      significantly down-regulated. KEGG enrichment analysis of cell signaling pathways 
      revealed that steroid hormone biosynthesis-related genes were enriched. 
      CONCLUSION: Among patients treated with GnRH antagonists, the significant 
      difference in the number of oocytes fertilized in vitro and the number of 
      available embryos are associated with the difference in the expression of genes 
      of ovarian granulosa cells.
FAU - Teng, Juan
AU  - Teng J
AD  - Department of Genetic and Laboratory Medicine, Linyi People's Hospital, Linyi, 
      Shandong 276000, China. 13869986938@126.com.
FAU - Wang, Ancong
AU  - Wang A
FAU - Zhao, Xiangyu
AU  - Zhao X
FAU - Liu, Meiju
AU  - Liu M
FAU - Wu, Yan
AU  - Wu Y
FAU - Li, Lin
AU  - Li L
FAU - Li, Wen
AU  - Li W
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
SB  - IM
MH  - Female
MH  - Humans
MH  - Pregnancy
MH  - Embryonic Development
MH  - Gene Expression
MH  - Gonadotropin-Releasing Hormone/antagonists & inhibitors
MH  - *Granulosa Cells
MH  - *Polycystic Ovary Syndrome/drug therapy/genetics
EDAT- 2022/12/02 06:00
MHDA- 2022/12/06 06:00
CRDT- 2022/12/01 09:14
PHST- 2022/12/01 09:14 [entrez]
PHST- 2022/12/02 06:00 [pubmed]
PHST- 2022/12/06 06:00 [medline]
AID - 940639252 [pii]
AID - 10.3760/cma.j.cn511374-20211109-00891 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2022 Dec 10;39(12):1354-1359. doi: 
      10.3760/cma.j.cn511374-20211109-00891.