PMID- 36454319
OWN - NLM
STAT- MEDLINE
DCOM- 20221205
LR  - 20230126
IS  - 1432-072X (Electronic)
IS  - 0302-8933 (Print)
IS  - 0302-8933 (Linking)
VI  - 205
IP  - 1
DP  - 2022 Dec 1
TI  - Genomic and metabolic features of Bacillus cereus, inhibiting the growth of 
      Sclerotinia sclerotiorum by synthesizing secondary metabolites.
PG  - 8
LID - 10.1007/s00203-022-03351-5 [doi]
LID - 8
AB  - We investigated the biocontrol mechanism of Bacillus cereus CF4-51 to find 
      powerful microbes that effectively control Sclerotinia sclerotiorum. To assess 
      its inhibitory effect on fungal growth, the plant pathogen (S. sclerotiorum) was 
      co-cultured with Bacillus cereus. Scanning electron microscope (SEM) was used to 
      study the morphology of S. sclerotiorum treated with CF4-51 biofumigant. The 
      expression of sclerotium formation-related genes was analyzed by qRT-PCR. We 
      performed whole genome sequencing of CF4-51 by PacBio Sequel platform. 
      Lipopeptides were extracted from strain CF4-51 according to the method of 
      hydrochloric acid precipitation and methanol dissolution. The volatiles CF4-51 
      were identified using gas chromatography-mass spectrometry (GC-MS). We found that 
      the volatile organic compounds (VOCs) released by CF4-51 damaged the S. 
      sclerotiorum hyphae and inhibited the formation of sclerotia. The qRT-PCR data 
      revealed the down-regulated expression of the genes involved in sclerotial 
      formation. Moreover, we analyzed the B. cereus CF4-51 genome and metabolites. The 
      genome consisted of 5.35 Mb, with a GC content of 35.74%. An examination of the 
      genome revealed the presence of several gene clusters for the biosynthesis of 
      antibiotics, siderophores, and various other bioactive compounds, including those 
      belonging to the NRPS-like, LAP, RIPP-like, NRPS, betalactone, CDPS, terpene, 
      ladderane, ranthipeptide, and lanthipeptide (class II) categories. A gas 
      chromatography-tandem mass spectrometry analysis identified 45 VOCs produced by 
      strain CF4-51. Among these, technical grade formulations of five were chosen for 
      further study: 2-Pentadecanone, 6,10,14-trimethyl-,1,2-Benzenedicarboxylic acid, 
      bis(2-methylpropyl) ester, Dibutyl phthalate, Cyclododecane, Heptadecane. the 
      five major constituents play important roles in the antifungal activity of the 
      VOCs CF4-51 on the growth of S. sclerotiorum. The secondary metabolites produced 
      by strain CF4-51are critical for the inhibition of S. sclerotiorum hyphal growth 
      and sclerotial formation.
CI  - (c) 2022. The Author(s).
FAU - Hu, Jinghan
AU  - Hu J
AD  - College of Horticulture and Plant Protection, Inner Mongolia Agricultural 
      University, Hohhot, 010020, Inner Mongolia, China.
FAU - Dong, Baozhu
AU  - Dong B
AD  - College of Horticulture and Plant Protection, Inner Mongolia Agricultural 
      University, Hohhot, 010020, Inner Mongolia, China.
AD  - Inner Mongolia Cold and Arid Region Crop Protection Engineering Technology 
      Center, Hohhot, 010020, Inner Mongolia, China.
FAU - Wang, Dong
AU  - Wang D
AD  - College of Horticulture and Plant Protection, Inner Mongolia Agricultural 
      University, Hohhot, 010020, Inner Mongolia, China.
AD  - Inner Mongolia Cold and Arid Region Crop Protection Engineering Technology 
      Center, Hohhot, 010020, Inner Mongolia, China.
FAU - Meng, Huanwen
AU  - Meng H
AD  - College of Horticulture and Plant Protection, Inner Mongolia Agricultural 
      University, Hohhot, 010020, Inner Mongolia, China.
AD  - Inner Mongolia Cold and Arid Region Crop Protection Engineering Technology 
      Center, Hohhot, 010020, Inner Mongolia, China.
FAU - Li, Xiaojuan
AU  - Li X
AD  - College of Horticulture and Plant Protection, Inner Mongolia Agricultural 
      University, Hohhot, 010020, Inner Mongolia, China.
FAU - Zhou, Hongyou
AU  - Zhou H
AD  - College of Horticulture and Plant Protection, Inner Mongolia Agricultural 
      University, Hohhot, 010020, Inner Mongolia, China. hongyouzhou2002@aliyun.com.
AD  - Inner Mongolia Cold and Arid Region Crop Protection Engineering Technology 
      Center, Hohhot, 010020, Inner Mongolia, China. hongyouzhou2002@aliyun.com.
LA  - eng
GR  - 2017YFD0201101/the earmarked fund for National Key R&D Projects/
GR  - 31260445/the National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20221201
PL  - Germany
TA  - Arch Microbiol
JT  - Archives of microbiology
JID - 0410427
RN  - 0 (Volatile Organic Compounds)
RN  - Sclerotinia sclerotiorum
SB  - IM
MH  - Bacillus cereus/genetics
MH  - Gas Chromatography-Mass Spectrometry
MH  - Genomics
MH  - *Ascomycota/genetics
MH  - *Volatile Organic Compounds
PMC - PMC9715469
OTO - NOTNLM
OT  - Antifungal activity
OT  - Bacillus cereus
OT  - Genome mining
OT  - Sclerotinia sclerotiorum
OT  - Secondary metabolites
COIS- The authors declare that they have no conflict of interest.
EDAT- 2022/12/02 06:00
MHDA- 2022/12/06 06:00
PMCR- 2022/12/01
CRDT- 2022/12/01 11:15
PHST- 2022/05/15 00:00 [received]
PHST- 2022/11/21 00:00 [accepted]
PHST- 2022/10/26 00:00 [revised]
PHST- 2022/12/01 11:15 [entrez]
PHST- 2022/12/02 06:00 [pubmed]
PHST- 2022/12/06 06:00 [medline]
PHST- 2022/12/01 00:00 [pmc-release]
AID - 10.1007/s00203-022-03351-5 [pii]
AID - 3351 [pii]
AID - 10.1007/s00203-022-03351-5 [doi]
PST - epublish
SO  - Arch Microbiol. 2022 Dec 1;205(1):8. doi: 10.1007/s00203-022-03351-5.