PMID- 36454668
OWN - NLM
STAT- MEDLINE
DCOM- 20230214
LR  - 20230214
IS  - 1522-7278 (Electronic)
IS  - 1520-4081 (Linking)
VI  - 38
IP  - 3
DP  - 2023 Mar
TI  - Molecular insights of 2,6-dichlorobenzoquinone-induced cytotoxicity in zebrafish 
      embryo: Activation of ROS-mediated cell cycle arrest and apoptosis.
PG  - 694-700
LID - 10.1002/tox.23721 [doi]
AB  - 2,6-dichloro-1,4-benzoquinone (2,6-DCBQ), as an emerging disinfection by-product, 
      has been frequently detected in waters, posing potential health risk on public 
      health. Although some studies have pointed out that 2,6-DCBQ exposure can induce 
      cytotoxicity, limited information is available for underlying mechanism for 
      2,6-DCBQ-induced cytotoxicity. To explore this mechanism, we assessed the levels 
      of reactive oxygen species (ROS), acridine orange (AO) staining, and the mRNA 
      transcriptions of genes (Chk2, Cdk2, Ccna, Ccnb and Ccne) involved in cell-cycle 
      and genes (p53, bax, bcl-2 and caspase 3) involved in apoptosis in zebrafish 
      embryo, after exposed to different concentrations (10, 30, 60, 90 and 120 mug/L) 
      of 2,6-DCBQ for 72 h. Our results indicated that 2,6-DCBQ exposure induced ROS 
      generation and cell apoptosis, and disturbed the mRNA transcription of genes 
      related to cell cycle and apoptosis in zebrafish embryo. Moreover, we also found 
      that 30 ~ 60 mug/L 2,6-DCBQ is the important transition from cell-cycle arrest to 
      cell apoptosis. These results provided novel insight into 2,6-DCBQ-induced 
      cytotoxicity.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Dong, Mingyue
AU  - Dong M
AD  - College of Geography and Environmental Science, Zhejiang Normal University, 
      Jinhua, China.
FAU - Ding, Yan
AU  - Ding Y
AD  - College of Geography and Environmental Science, Zhejiang Normal University, 
      Jinhua, China.
FAU - Liu, Yingying
AU  - Liu Y
AD  - College of Geography and Environmental Science, Zhejiang Normal University, 
      Jinhua, China.
FAU - Xu, Zeqiong
AU  - Xu Z
AD  - College of Geography and Environmental Science, Zhejiang Normal University, 
      Jinhua, China.
FAU - Hong, Huachang
AU  - Hong H
AD  - College of Geography and Environmental Science, Zhejiang Normal University, 
      Jinhua, China.
FAU - Sun, Hongjie
AU  - Sun H
AUID- ORCID: 0000-0003-0835-5888
AD  - College of Geography and Environmental Science, Zhejiang Normal University, 
      Jinhua, China.
FAU - Huang, Xianfeng
AU  - Huang X
AD  - National and Local Joint Engineering Research Center for Ecological Treatment 
      Technology of Urban Water Pollution, College of Life and Environmental Science, 
      Wenzhou University, Wenzhou, China.
FAU - Yu, Xinwei
AU  - Yu X
AD  - Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, Zhoushan 
      Municipal Center for Disease Control and Prevention, Zhoushan, China.
FAU - Chen, Qiang
AU  - Chen Q
AD  - Department of Environmental and Occupational Health, Jinhua Center for Disease 
      Control and Prevention, Jinhua, People's Republic of China.
LA  - eng
GR  - LGF19B070007/Public Welfare Technology Projects of Zhejiang Province/
GR  - 2020-4-084/Jinhua Public Welfare Technology Application Research Project/
PT  - Journal Article
DEP - 20221201
PL  - United States
TA  - Environ Toxicol
JT  - Environmental toxicology
JID - 100885357
RN  - 697-91-6 (2,6-dichlorobenzoquinone)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Reactive Oxygen Species/metabolism
MH  - *Zebrafish/metabolism
MH  - *Apoptosis/genetics
MH  - Cell Cycle Checkpoints
MH  - RNA, Messenger/metabolism
OTO - NOTNLM
OT  - 2,6-dichlorobenzoquinone
OT  - apoptosis
OT  - cell cycle
OT  - reactive oxygen species
OT  - zebrafish
EDAT- 2022/12/02 06:00
MHDA- 2023/02/15 06:00
CRDT- 2022/12/01 12:43
PHST- 2022/11/18 00:00 [revised]
PHST- 2022/08/25 00:00 [received]
PHST- 2022/11/20 00:00 [accepted]
PHST- 2022/12/02 06:00 [pubmed]
PHST- 2023/02/15 06:00 [medline]
PHST- 2022/12/01 12:43 [entrez]
AID - 10.1002/tox.23721 [doi]
PST - ppublish
SO  - Environ Toxicol. 2023 Mar;38(3):694-700. doi: 10.1002/tox.23721. Epub 2022 Dec 1.