PMID- 36462110
OWN - NLM
STAT- MEDLINE
DCOM- 20230504
LR  - 20230504
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 195
IP  - 5
DP  - 2023 May
TI  - Antifungal Activity of Camelus-Derived LFA-LFC Chimeric Peptide Gelatin Film and 
      Effect on Oral Bacterial Biofilm.
PG  - 2993-3010
LID - 10.1007/s12010-022-04248-1 [doi]
AB  - The objective of this study was to construct lactoferrin (LFA-LFC) chimeric 
      peptide gelatin drug-loaded fiber film by tissue engineering strategy, and study 
      its bacteriostatic effect on oral pathogens (especially Candida albicans) and its 
      effect on biofilm. First of all, LFA-LFC chitosan nanoparticles were prepared 
      firstly, and then fluconazole gelatin (LF/GH/F) film loaded with LFA-LFC was 
      prepared by electrospinning. Scanning electron microscope (SEM), mechanical 
      strength, drug release, cytotoxicity, and real-time PCR were used to test the 
      properties of the synthesized materials. SEM showed that there was the reticular 
      structure for fiber film before and after cross-linking. LF/GH/F film had no 
      obvious cytotoxicity, with good biocompatibility and drug release; real-time PCR 
      and antibacterial test showed that the LF/GH/F film had good antibacterial 
      activity. LF/GH/F film has a good inhibitory effect on oral pathogens, and its 
      mechanism is related to biofilm. The antibacterial experiments of nanofiber 
      membrane in vitro and the effect of bacterial biofilm were carried out. The 
      effect of LF/GH/F on oral microbial flora structure was studied by fluorescence 
      quantitative pest techniques.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Li, Chen
AU  - Li C
AD  - Department of Oral Medicine, School and Hospital of Stomatology, Jilin 
      University, Changchun, 130021, People's Republic of China. chenl0711@163.com.
AD  - State Key Laboratory of Supramolecular Structure and Materials, College of 
      Chemistry, Jilin University, Changchun, 130012, People's Republic of China. 
      chenl0711@163.com.
FAU - Wang, Dandan
AU  - Wang D
AD  - Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Jilin 
      University, Changchun, 130021, People's Republic of China.
AD  - Department of Oral Pathology, School and Hospital of Stomatology, Jilin 
      University, Changchun, 130021, People's Republic of China.
FAU - Zhou, Yang
AU  - Zhou Y
AD  - Department of Oral Medicine, School and Hospital of Stomatology, Jilin 
      University, Changchun, 130021, People's Republic of China.
FAU - Kang, Kai
AU  - Kang K
AD  - Department of Oral Medicine, School and Hospital of Stomatology, Jilin 
      University, Changchun, 130021, People's Republic of China.
FAU - Ge, Yan
AU  - Ge Y
AD  - Department of Oral Medicine, School and Hospital of Stomatology, Jilin 
      University, Changchun, 130021, People's Republic of China.
LA  - eng
GR  - 2018M631887/Postdoctoral Science Foundation of China/
PT  - Journal Article
DEP - 20221203
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Antifungal Agents)
RN  - 0 (gelatin film)
RN  - 9000-70-8 (Gelatin)
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Peptides)
SB  - IM
MH  - Animals
MH  - *Antifungal Agents/pharmacology
MH  - *Gelatin/chemistry
MH  - Camelus
MH  - Biofilms
MH  - Anti-Bacterial Agents/pharmacology/chemistry
MH  - Peptides/pharmacology
OTO - NOTNLM
OT  - Antibacterial activity
OT  - Biofilm
OT  - Fiberfilm
OT  - Fluconazole
OT  - Gelatin film
OT  - LFA-LFC
OT  - Oral pathogens
EDAT- 2022/12/04 06:00
MHDA- 2023/05/04 12:42
CRDT- 2022/12/03 11:15
PHST- 2022/11/08 00:00 [accepted]
PHST- 2023/05/04 12:42 [medline]
PHST- 2022/12/04 06:00 [pubmed]
PHST- 2022/12/03 11:15 [entrez]
AID - 10.1007/s12010-022-04248-1 [pii]
AID - 10.1007/s12010-022-04248-1 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2023 May;195(5):2993-3010. doi: 
      10.1007/s12010-022-04248-1. Epub 2022 Dec 3.