PMID- 36463679
OWN - NLM
STAT- MEDLINE
DCOM- 20230105
LR  - 20230111
IS  - 1879-2448 (Electronic)
IS  - 0043-1354 (Linking)
VI  - 229
DP  - 2023 Feb 1
TI  - Continuous cultivation of Dehalococcoides mccartyi with brominated tyrosine 
      avoids toxic byproducts and gives tight reactor control.
PG  - 119396
LID - S0043-1354(22)01341-0 [pii]
LID - 10.1016/j.watres.2022.119396 [doi]
AB  - Dehalococcoides mccartyi strain CBDB1 is a strictly anaerobic 
      organohalide-respiring bacterium with strong application potential to remediate 
      aquifers and soils contaminated with halogenated aromatics. To date, cultivation 
      of strain CBDB1 has mostly been done in bottles or fed-batch reactors. Challenges 
      with such systems include low biomass yield and difficulties in controlling the 
      growth conditions. Here, we report the cultivation of planktonic D. mccartyi 
      strain CBDB1 in a continuous stirring tank reactor (CSTR) that led to high cell 
      densities ( approximately 8 x 10(8) cells mL(-1)) and dominance of strain CBDB1. The reactor 
      culture received acetate, hydrogen, and the brominated amino acid D- or 
      L-3,5-dibromotyrosine as substrates. Both D- and L-3,5-dibromotyrosine were 
      utilized as respiratory electron acceptors and are promising for biomass 
      production due to their decent solubility in water and the formation of a 
      non-toxic debromination product, tyrosine. By monitoring headspace pressure 
      decrease which is indicative of hydrogen consumption, the organohalide 
      respiration rate was followed in real time. Proteomics analyses revealed that the 
      reductive dehalogenase CbdbA238 was highly expressed with both D- and 
      L-3,5-dibromotyrosine, while other reductive dehalogenases including those that 
      were previously suggested to be constitutively expressed, were repressed. 
      Denaturing gradient gel electrophoresis (DGGE) of amplified 16S rRNA genes 
      indicated that the majority of cells in the community belonged to the 
      Dehalococcoides although the CSTR was operated under non-sterile conditions. 
      Hence, tightly controlled CSTR cultivation of Dehalococcoides opens novel options 
      to improve biomass production for bioaugmentation and for advanced biochemical 
      studies.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Reino, Clara
AU  - Reino C
AD  - Helmholtz Centre for Environmental Research - UFZ, Department of Environmental 
      Biotechnology, Permoserstrasse 15, 04318, Leipzig, Germany.
FAU - Ding, Chang
AU  - Ding C
AD  - Helmholtz Centre for Environmental Research - UFZ, Department of Environmental 
      Biotechnology, Permoserstrasse 15, 04318, Leipzig, Germany. Electronic address: 
      chang.ding@ufz.de.
FAU - Adrian, Lorenz
AU  - Adrian L
AD  - Helmholtz Centre for Environmental Research - UFZ, Department of Environmental 
      Biotechnology, Permoserstrasse 15, 04318, Leipzig, Germany; Chair of 
      Geobiotechnology, Technische Universitat Berlin, Ackerstrasse 76, 13355, Berlin, 
      Germany.
LA  - eng
PT  - Journal Article
DEP - 20221119
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 42HK56048U (Tyrosine)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - Dehalococcoides mccartyi
SB  - IM
MH  - *Tyrosine
MH  - RNA, Ribosomal, 16S
MH  - Hydrogen
MH  - *Chloroflexi/genetics
MH  - Biodegradation, Environmental
OTO - NOTNLM
OT  - Bioremediation
OT  - Brominated phenolic compounds
OT  - Continuous stirred tank reactor
OT  - Online activity monitoring
OT  - Organohalide respiration
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/12/05 06:00
MHDA- 2023/01/06 06:00
CRDT- 2022/12/04 18:17
PHST- 2022/07/08 00:00 [received]
PHST- 2022/11/18 00:00 [revised]
PHST- 2022/11/18 00:00 [accepted]
PHST- 2022/12/05 06:00 [pubmed]
PHST- 2023/01/06 06:00 [medline]
PHST- 2022/12/04 18:17 [entrez]
AID - S0043-1354(22)01341-0 [pii]
AID - 10.1016/j.watres.2022.119396 [doi]
PST - ppublish
SO  - Water Res. 2023 Feb 1;229:119396. doi: 10.1016/j.watres.2022.119396. Epub 2022 
      Nov 19.