PMID- 36466180
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221206
IS  - 1662-4548 (Print)
IS  - 1662-453X (Electronic)
IS  - 1662-453X (Linking)
VI  - 16
DP  - 2022
TI  - Ultrastructural destruction of neurovascular unit in experimental cervical 
      spondylotic myelopathy.
PG  - 1031180
LID - 10.3389/fnins.2022.1031180 [doi]
LID - 1031180
AB  - BACKGROUND AND PURPOSE: The pathogenesis of cervical spondylotic myelopathy (CSM) 
      remains unclear. This study aimed to explore the ultrastructural pathology of 
      neurovascular unit (NVU) during natural development of CSM. METHODS: A total of 
      24 rats were randomly allocated to the control group and the CSM group. 
      Basso-Beattie-Bresnahan (BBB) scoring and somatosensory evoked potentials (SEP) 
      were used as functional assessments. Hematoxylin-eosin (HE), toluidine blue (TB), 
      and Luxol fast blue (LFB) stains were used for general structure observation. 
      Transmission electron microscopy (TEM) was applied for investigating 
      ultrastructural characteristics. RESULTS: The evident compression caused 
      significant neurological dysfunction, which was confirmed by the decrease in BBB 
      score and SEP amplitude, as well as the prolongation of SEP latency (P < 0.05). 
      The histopathological findings verified a significant decrease in the amount of 
      Nissl body and myelin area and an increase in vacuolation compared with the 
      control group (P < 0.05). The TEM results revealed ultrastructural destruction of 
      NVU in several forms, including: neuronal degeneration and apoptosis; disruption 
      of axonal cytoskeleton (neurofilaments) and myelin sheath and dystrophy of axonal 
      terminal with dysfunction mitochondria; degenerative oligodendrocyte, astrocyte, 
      and microglial cell inclusions with degenerating axon and dystrophic dendrite; 
      swollen microvascular endothelium and loss of tight junction integrity; corroded 
      basement membrane and collapsed microvascular wall; and proliferated pericyte and 
      perivascular astrocytic endfeet. In the CSM group, reduction was observed in the 
      amount of mitochondria with normal appearance and the number of cristae per 
      mitochondria (P < 0.05), while no substantial drop of synaptic vesicle number was 
      seen (P > 0.05). Significant narrowing of microvascular lumen size was also 
      observed, accompanied by growth in the vascular wall area, endothelial area, 
      basement membrane thickness, astrocytic endfeet area, and pericyte coverage area 
      (rate) (P < 0.05). CONCLUSION: Altogether, the findings of this study 
      demonstrated ultrastructural destruction of NVU in an experimental CSM model with 
      dorsal-lateral compression, revealing one of the crucial pathophysiological 
      mechanisms of CSM.
CI  - Copyright (c) 2022 Li, Wang, Tan, Wang, Hu and Hu.
FAU - Li, Guang-Sheng
AU  - Li GS
AD  - Spinal Division of Orthopaedic and Traumatology Center, The Affiliated Hospital 
      of Guangdong Medical University, Zhanjiang, China.
FAU - Wang, Xu-Xiang
AU  - Wang XX
AD  - Spinal Division of Orthopaedic and Traumatology Center, The Affiliated Hospital 
      of Guangdong Medical University, Zhanjiang, China.
FAU - Tan, Ron-Bang
AU  - Tan RB
AD  - Spinal Division of Orthopaedic and Traumatology Center, The Affiliated Hospital 
      of Guangdong Medical University, Zhanjiang, China.
FAU - Wang, Kang-Heng
AU  - Wang KH
AD  - Spinal Division of Orthopaedic and Traumatology Center, The Affiliated Hospital 
      of Guangdong Medical University, Zhanjiang, China.
AD  - Department of Orthopaedics and Traumatology, The University of Hong Kong-Shenzhen 
      Hospital, Shenzhen, China.
FAU - Hu, Xiao-Song
AU  - Hu XS
AD  - Spinal Division of Orthopaedic and Traumatology Center, The Affiliated Hospital 
      of Guangdong Medical University, Zhanjiang, China.
AD  - Department of Orthopaedics and Traumatology, The University of Hong Kong-Shenzhen 
      Hospital, Shenzhen, China.
FAU - Hu, Yong
AU  - Hu Y
AD  - Spinal Division of Orthopaedic and Traumatology Center, The Affiliated Hospital 
      of Guangdong Medical University, Zhanjiang, China.
AD  - Department of Orthopaedics and Traumatology, The University of Hong Kong-Shenzhen 
      Hospital, Shenzhen, China.
AD  - Department of Orthopaedics and Traumatology, The University of Hong Kong, Hong 
      Kong, Hong Kong SAR, China.
LA  - eng
PT  - Journal Article
DEP - 20221116
PL  - Switzerland
TA  - Front Neurosci
JT  - Frontiers in neuroscience
JID - 101478481
PMC - PMC9709118
OTO - NOTNLM
OT  - cervical spondylotic myelopathy
OT  - chronic
OT  - compressive
OT  - neurovascular unit
OT  - spinal cord injury
OT  - ultrastructural evidence
OT  - ultrastructural pathology
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/12/06 06:00
MHDA- 2022/12/06 06:01
PMCR- 2022/01/01
CRDT- 2022/12/05 03:57
PHST- 2022/08/29 00:00 [received]
PHST- 2022/10/25 00:00 [accepted]
PHST- 2022/12/05 03:57 [entrez]
PHST- 2022/12/06 06:00 [pubmed]
PHST- 2022/12/06 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fnins.2022.1031180 [doi]
PST - epublish
SO  - Front Neurosci. 2022 Nov 16;16:1031180. doi: 10.3389/fnins.2022.1031180. 
      eCollection 2022.