PMID- 36467728
OWN - NLM
STAT- MEDLINE
DCOM- 20221206
LR  - 20221206
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 12
DP  - 2022
TI  - Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show 
      modulatory properties and confer resistance to lethal infection as a cell-free 
      based therapy strategy.
PG  - 980817
LID - 10.3389/fcimb.2022.980817 [doi]
LID - 980817
AB  - Extracellular vesicles (EVs) include a heterogeneous group of particles. 
      Microvesicles, apoptotic bodies and exosomes are the most characterized vesicles. 
      They can be distinguished by their size, morphology, origin and molecular 
      composition. To date, increasing studies demonstrate that EVs mediate 
      intercellular communication. EVs reach considerable interest in the scientific 
      community due to their role in diverse processes including antigen-presentation, 
      stimulation of anti-tumoral immune responses, tolerogenic or inflammatory 
      effects. In pathogens, EV shedding is well described in fungi, bacteria, 
      protozoan and helminths parasites. For Trypanosoma cruzi EV liberation and 
      protein composition was previously described. Dendritic cells (DCs), among other 
      cells, are key players promoting the immune response against pathogens and also 
      maintaining self-tolerance. In previous reports we have demonstrate that T. cruzi 
      downregulates DCs immunogenicity in vitro and in vivo. Here we analyze EVs from 
      the in vitro interaction between blood circulating trypomastigotes (Tp) and 
      bone-marrow-derived DCs. We found that Tp incremented the number and the size of 
      EVs in cultures with DCs. EVs displayed some exosome markers and intracellular 
      RNA. Protein analysis demonstrated that the parasite changes the DC protein-EV 
      profile. We observed that EVs from the interaction of Tp-DCs were easily captured 
      by unstimulated-DCs in comparison with EVs from DCs cultured without the 
      parasite, and also modified the activation status of LPS-stimulated DCs. 
      Noteworthy, we found protection in animals treated with EVs-DCs+Tp and challenged 
      with T. cruzi lethal infection. Our goal is to go deep into the molecular 
      characterization of EVs from the DCs-Tp interaction, in order to identify 
      mediators for therapeutic purposes.
CI  - Copyright (c) 2022 Gutierrez, Ancarola, Volpato-Rossi, Marcilla, Ramirez, 
      Rosenzvit, Cucher and Poncini.
FAU - Gutierrez, Brenda Celeste
AU  - Gutierrez BC
AD  - Instituto de Investigaciones en Microbiologia y Parasitologia Medicas (IMPaM), 
      Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos 
      Aires, Argentina.
FAU - Ancarola, Maria Eugenia
AU  - Ancarola ME
AD  - Instituto de Investigaciones en Microbiologia y Parasitologia Medicas (IMPaM), 
      Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos 
      Aires, Argentina.
FAU - Volpato-Rossi, Izadora
AU  - Volpato-Rossi I
AD  - Programa de Pos-graduacao em Biologia Celular e Molecular, Universidade Federal 
      do Parana, Curitiba, Parana, Brazil.
AD  - Instituto Carlos Chagas - Fiocruz Parana, Curitiba, Parana, Brazil.
FAU - Marcilla, Antonio
AU  - Marcilla A
AD  - Departamento de Farmacia y Tecnologia Farmaceutica y Parasitologia, Universitat 
      de Valencia, Valencia, Spain.
FAU - Ramirez, Marcel Ivan
AU  - Ramirez MI
AD  - Programa de Pos-graduacao em Biologia Celular e Molecular, Universidade Federal 
      do Parana, Curitiba, Parana, Brazil.
AD  - Instituto Carlos Chagas - Fiocruz Parana, Curitiba, Parana, Brazil.
FAU - Rosenzvit, Mara Cecilia
AU  - Rosenzvit MC
AD  - Instituto de Investigaciones en Microbiologia y Parasitologia Medicas (IMPaM), 
      Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos 
      Aires, Argentina.
FAU - Cucher, Marcela
AU  - Cucher M
AD  - Instituto de Investigaciones en Microbiologia y Parasitologia Medicas (IMPaM), 
      Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos 
      Aires, Argentina.
FAU - Poncini, Carolina Veronica
AU  - Poncini CV
AD  - Instituto de Investigaciones en Microbiologia y Parasitologia Medicas (IMPaM), 
      Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos 
      Aires, Argentina.
AD  - Departamento de Microbiologia, Facultad de Medicina, Universidad de Buenos Aires 
      (UBA), Buenos Aires, Argentina.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221116
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
SB  - IM
MH  - Animals
MH  - *Trypanosoma cruzi
MH  - *Extracellular Vesicles
MH  - Cell Communication
MH  - *Chagas Disease/therapy
MH  - *Exosomes
PMC - PMC9710384
OTO - NOTNLM
OT  - T. cruzi
OT  - cell free therapy
OT  - dendric cells (DCs)
OT  - dendritic cells
OT  - extracellular vesicles (EVs)
OT  - immunotherapy
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/12/06 06:00
MHDA- 2022/12/07 06:00
PMCR- 2022/01/01
CRDT- 2022/12/05 04:31
PHST- 2022/06/28 00:00 [received]
PHST- 2022/10/20 00:00 [accepted]
PHST- 2022/12/05 04:31 [entrez]
PHST- 2022/12/06 06:00 [pubmed]
PHST- 2022/12/07 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2022.980817 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2022 Nov 16;12:980817. doi: 
      10.3389/fcimb.2022.980817. eCollection 2022.