PMID- 36467921 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20221206 IS - 2470-1343 (Electronic) IS - 2470-1343 (Linking) VI - 7 IP - 47 DP - 2022 Nov 29 TI - Radix Pueraria Flavonoids Assisted Green Synthesis of Reduced Gold Nanoparticles: Application for Electrochemical Nonenzymatic Detection of Cholesterol in Food Samples. PG - 43045-43054 LID - 10.1021/acsomega.2c05358 [doi] AB - Using radix pueraria flavonoids (RPFs) as a reducing and stabilizing agent, we report a simple, cost-effective, and ecologically friendly green synthesis technique for gold nanoparticles (AuNPs) in the present study. Ultraviolet-visible (UV) spectroscopy, transmission electron microscopy (TEM), Fourier transform infrared (FTIR), and X-ray diffraction (XRD) investigations were used to characterize the AuNPs. The results demonstrated that the produced AuNPs were nearly spherical and that their particle sizes had a mean diameter of 4.85 +/- 0.75 nm. The "Green" AuNPs, exhibiting remarkable peroxidase-like activity and Michaelis-Menten kinetics with high affinity for H(2)O(2) and 3,3',5,5'-tetramethylbenzidine (TMB), were effectively applied to the fabrication of a sensitive nonenzymatic enhanced electrochemical sensor for the detection of cholesterol (Cho). Under optimum circumstances, it was possible to establish two linear ranges of 1-100 and 250-5000 mumol/L with a detection limit of 0.259 mumol/L (signal/noise ratio (S/N) = 3). The suggested sensor was utilized with satisfactory findings to determine the amount of Cho in food samples. CI - (c) 2022 The Authors. Published by American Chemical Society. FAU - Han, Bolin AU - Han B AD - College of Food Engineering, Harbin University of Commerce, Harbin 150028, China. FAU - Guan, Huanan AU - Guan H AUID- ORCID: 0000-0003-1182-8180 AD - College of Food Engineering, Harbin University of Commerce, Harbin 150028, China. FAU - Song, Yan AU - Song Y AD - College of Food Engineering, Harbin University of Commerce, Harbin 150028, China. FAU - Liu, Ying AU - Liu Y AD - College of Food Engineering, Harbin University of Commerce, Harbin 150028, China. LA - eng PT - Journal Article DEP - 20221116 PL - United States TA - ACS Omega JT - ACS omega JID - 101691658 PMC - PMC9713785 COIS- The authors declare no competing financial interest. EDAT- 2022/12/06 06:00 MHDA- 2022/12/06 06:01 PMCR- 2022/11/16 CRDT- 2022/12/05 04:34 PHST- 2022/08/20 00:00 [received] PHST- 2022/11/04 00:00 [accepted] PHST- 2022/12/05 04:34 [entrez] PHST- 2022/12/06 06:00 [pubmed] PHST- 2022/12/06 06:01 [medline] PHST- 2022/11/16 00:00 [pmc-release] AID - 10.1021/acsomega.2c05358 [doi] PST - epublish SO - ACS Omega. 2022 Nov 16;7(47):43045-43054. doi: 10.1021/acsomega.2c05358. eCollection 2022 Nov 29.