PMID- 36471945
OWN - NLM
STAT- MEDLINE
DCOM- 20221207
LR  - 20221207
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 47
IP  - 22
DP  - 2022 Nov
TI  - [Mechanism of Cyathulae Radix in treatment of knee osteoarthritis based on 
      metabolomics].
PG  - 6199-6206
LID - 10.19540/j.cnki.cjcmm.20220706.703 [doi]
AB  - Metabolomics based on ultra-high performance liquid chromatography-tandem 
      quadrupole time-of-flight mass spectrometry(UHPLC-Q-TOF-MS) was employed to 
      analyze the effect of Cyathulae Radix on serum and urine in rats with knee 
      osteoarthritis, and to decipher the mechanism of Cyathulae Radix in the treatment 
      of knee osteoarthritis. SD rats were randomized into a normal group, a model 
      group, a positive drug group, and a Cyathulae Radix group. The knee 
      osteoarthritis model was established by injecting 2% pa-pain and 0.03 mol.L~(-1) 
      cysteine, and the serum levels of tumor necrosis factor-alpha(TNF-alpha) and matrix 
      metalloproteinase-3(MMP-3) in the model group was measured to preliminarily 
      evaluate the therapeutic effect of Cyathulae Radix on knee osteoarthritis. 
      UHPLC-Q-TOF-MS was employed to establish the metabolic profile of endogenous 
      small molecule metabolites in the four groups. Potential biomarkers were screened 
      out by multivariate analysis methods such as partial least squares discriminant 
      analysis(PLS-DA) and orthogonal partial least squares discriminant 
      analysis(OPLS-DA) in combination with t-test, variable importance for 
      projection(VIP), and fold-change. The related metabolic pathways were enriched 
      with the help of MetaboAnalyst 5.0. The results showed that Cyathulae Radix 
      alleviated the general signs of rats with knee osteoarthritis, and reduced the 
      levels of TNF-alpha and MMP-3 in the rat serum. Twenty-eight differential metabolites 
      that might be associated with the therapeutic effect of Cyathulae Radix were 
      screened out from serum and urine. They were mainly involved in arginine 
      biosynthesis, nicotinate and nicotinamide metabolism, tricarboxylic acid cycle, 
      alanine, aspartate and glutamate metabolism, riboflavin metabolism, glyoxylate 
      and dicarboxylate metabolism, and pyrimidine metabolism. Through metabonomics 
      analysis, this study predicted the possible mechanism of Cyathulae Radix in the 
      treatment of knee osteoarthritis, which laid a foundation for further research.
FAU - Liu, Shuang-Jing
AU  - Liu SJ
AD  - School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China.
FAU - Li, Jian-Peng
AU  - Li JP
AD  - School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China.
FAU - Wang, Yan-Zhi
AU  - Wang YZ
AD  - School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China 
      Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases 
      Co-constructed by Henan Province & Education Ministry of P.R.China Zhengzhou 
      450046, China.
FAU - Li, Kai-Zhi
AU  - Li KZ
AD  - School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China.
FAU - Zhu, Yue-Tong
AU  - Zhu YT
AD  - School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China.
FAU - Feng, Wei-Sheng
AU  - Feng WS
AD  - School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China 
      Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases 
      Co-constructed by Henan Province & Education Ministry of P.R.China Zhengzhou 
      450046, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Matrix Metalloproteinase 3
MH  - *Osteoarthritis, Knee/drug therapy
MH  - Tumor Necrosis Factor-alpha
MH  - Rats, Sprague-Dawley
MH  - Metabolomics/methods
MH  - Chromatography, High Pressure Liquid/methods
MH  - Biomarkers
OTO - NOTNLM
OT  - Cyathulae Radix
OT  - knee osteoarthritis
OT  - metabonomics
OT  - potential biomarker
EDAT- 2022/12/07 06:00
MHDA- 2023/02/25 06:00
CRDT- 2022/12/06 02:32
PHST- 2022/12/06 02:32 [entrez]
PHST- 2022/12/07 06:00 [pubmed]
PHST- 2023/02/25 06:00 [medline]
AID - 10.19540/j.cnki.cjcmm.20220706.703 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2022 Nov;47(22):6199-6206. doi: 
      10.19540/j.cnki.cjcmm.20220706.703.