PMID- 36472132
OWN - NLM
STAT- MEDLINE
DCOM- 20230301
LR  - 20230301
IS  - 2047-4849 (Electronic)
IS  - 2047-4830 (Linking)
VI  - 11
IP  - 5
DP  - 2023 Feb 28
TI  - Engineered cell-overexpression of circular RNA hybrid hydrogels promotes healing 
      of calvarial defects.
PG  - 1665-1676
LID - 10.1039/d2bm01472f [doi]
AB  - Craniomaxillofacial bone defects seriously affect the physical and mental health 
      of patients. Bone marrow mesenchymal stem cells (BMSCs) are "gold standard" cells 
      used for bone repair. However, the collection of BMSCs is invasive, and the 
      osteogenic capacity is limited with age. Human umbilical cord mesenchymal stem 
      cells (hUCMSCs) are promising alternative seed cells for bone tissue engineering. 
      Our group previously used high-throughput sequencing technology and 
      bioinformatics methods to detect circ-CTTN (hsa-circ_0003376) molecules, which 
      may play an essential role in the osteogenic differentiation of hUCMSCs. In this 
      study, osteogenic induction in vitro showed that the overexpressing circ-CTTN (OE 
      group) exhibits a more pronounced osteogenic phenotype. The levels of 
      osteogenesis-related genes in the OE group were highly expressed. The 
      gelatin-methacrylate (GelMA) hydrogel possessed excellent biocompatibility and 
      was used to load hUCMSCs. In the rat calvarial defect, the OE group presented a 
      larger bone healing volume and denser bone trabecular distribution than other 
      groups. So far, the overexpression of circ-CTTN could enhance the osteogenic 
      differentiation of hUCMSCs and accelerate bone reconstruction. Our research could 
      provide a new strategy and a strong theoretical basis for promoting hUCMSC 
      clinical application in bone tissue engineering.
FAU - Zhang, Min
AU  - Zhang M
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases & Med-X Center for Materials, West China Hospital of Stomatology, 
      Sichuan University, China. liaojinfeng.762@163.com.
AD  - Department of Oral Prosthodontics & Jinjiang out-patient section, West China 
      Hospital of Stomatology, Sichuan University, China.
FAU - He, Yanjing
AU  - He Y
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases & Med-X Center for Materials, West China Hospital of Stomatology, 
      Sichuan University, China. liaojinfeng.762@163.com.
AD  - Department of Oral Prosthodontics & Jinjiang out-patient section, West China 
      Hospital of Stomatology, Sichuan University, China.
FAU - Zhang, Xu
AU  - Zhang X
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases & Med-X Center for Materials, West China Hospital of Stomatology, 
      Sichuan University, China. liaojinfeng.762@163.com.
FAU - Gan, Shuaiqi
AU  - Gan S
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases & Med-X Center for Materials, West China Hospital of Stomatology, 
      Sichuan University, China. liaojinfeng.762@163.com.
AD  - Department of Oral Prosthodontics & Jinjiang out-patient section, West China 
      Hospital of Stomatology, Sichuan University, China.
FAU - Xie, Xi
AU  - Xie X
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases & Med-X Center for Materials, West China Hospital of Stomatology, 
      Sichuan University, China. liaojinfeng.762@163.com.
FAU - Zheng, Zheng
AU  - Zheng Z
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases & Med-X Center for Materials, West China Hospital of Stomatology, 
      Sichuan University, China. liaojinfeng.762@163.com.
AD  - Department of Oral Prosthodontics & Jinjiang out-patient section, West China 
      Hospital of Stomatology, Sichuan University, China.
FAU - Liao, Jinfeng
AU  - Liao J
AUID- ORCID: 0000-0001-8101-4030
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases & Med-X Center for Materials, West China Hospital of Stomatology, 
      Sichuan University, China. liaojinfeng.762@163.com.
FAU - Chen, Wenchuan
AU  - Chen W
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases & Med-X Center for Materials, West China Hospital of Stomatology, 
      Sichuan University, China. liaojinfeng.762@163.com.
AD  - Department of Oral Prosthodontics & Jinjiang out-patient section, West China 
      Hospital of Stomatology, Sichuan University, China.
LA  - eng
PT  - Journal Article
DEP - 20230228
PL  - England
TA  - Biomater Sci
JT  - Biomaterials science
JID - 101593571
RN  - 0 (RNA, Circular)
RN  - 0 (Hydrogels)
RN  - 0 (Cttn protein, rat)
RN  - 0 (Cortactin)
SB  - IM
MH  - Humans
MH  - Rats
MH  - Animals
MH  - *Osteogenesis/genetics
MH  - *Bone Regeneration/genetics
MH  - RNA, Circular
MH  - Hydrogels
MH  - Bone and Bones
MH  - Cell Differentiation/genetics
MH  - Cortactin
EDAT- 2022/12/07 06:00
MHDA- 2023/03/03 06:00
CRDT- 2022/12/06 03:52
PHST- 2022/12/07 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2022/12/06 03:52 [entrez]
AID - 10.1039/d2bm01472f [doi]
PST - epublish
SO  - Biomater Sci. 2023 Feb 28;11(5):1665-1676. doi: 10.1039/d2bm01472f.