PMID- 36478348 OWN - NLM STAT- MEDLINE DCOM- 20230717 LR - 20230718 IS - 1699-5848 (Electronic) IS - 0213-3911 (Linking) VI - 38 IP - 8 DP - 2023 Aug TI - Localization of Fusobacterium nucleatum in oral squamous cell carcinoma and its possible directly interacting protein molecules: A case series. PG - 929-939 LID - 10.14670/HH-18-560 [doi] AB - INTRODUCTION: While 15 to 20% of cancers are associated with microbial infection, the relationship between oral microorganisms and oral squamous cell carcinoma (OSCC) remains unclear. The location of bacteria in a tumor is closely related to its carcinogenic mechanism. The aim of this study was to analyse bacterial diversity in clinical OSCC tissue samples and tumor distant normal tissues, locate target bacteria, and search for proteins that may interact with target bacteria. MATERIALS AND METHODS: The 16S rDNA method was used to analyse bacterial diversity in clinical OSCC tissue samples and tumor distant normal tissues. Correlations between Fusobacterium abundance and clinicopathological characteristics were analysed using the chi2 test. The position of target bacteria was analysed by fluorescence in situ hybridization (FISH), and the expression of CK, CD31, CD45, CD68, cyclin D1, beta-catenin, E-cadherin, NF-kappaB, and HIF-1alpha was analysed by immunohistochemistry (IHC) in OSCC tumor tissues and tumor distant normal tissues. RESULTS: The 16S rDNA results showed that the detected amount of Fusobacterium in OSCC tumor tissues was significantly larger than that in tumor distant normal tissues. High expression of Fusobacterium was significantly correlated with the lifestyle-related oral risk habits, including smoking (p=0.036) and alcohol consumption (p=0.022), but did not correlate with patient sex, age, tumor laterality, tumor size, grade or TNM stage. Fusobacterium nucleatum was enriched in tumor stroma, where CD31+ blood vessels and inflammatory cells (including CD45+ leukocytes and CD68+ macrophages) were densely distributed. Cyclin D1 was mainly expressed in the nucleus of tumor cells. beta-catenin was expressed in the tumor cell membrane and was positively expressed in tumor interstitial vascular endothelial cells. E-cadherin was mainly expressed in tumor cell membranes. NF-kappaB was positively expressed in the cytoplasm of tumor cells, tumor interstitial cells and myo-fibrocytes. HIF-1alpha was mainly expressed in the cytoplasm of tumor interstitial cells. HIF-1alpha was highly expressed where Fusobacterium nucleatum was densely distributed. CONCLUSION: According to our study, the detected amount of Fusobacterium in OSCC tumor tissues was significantly larger than that in tumor distant normal tissues, and Fusobacterium nucleatum might aggravate inflammation and hypoxia by interacting with NF-kappaB and HIF-1alpha in OSCC. CI - (c)The Author(s) 2023. Open Access. This article is licensed under a Creative Commons CC-BY International License. FAU - He, Xuan AU - He X AD - Department of Operative Dentistry and Endodontology, College of Stomatology, Hospital of Stomatology, Guangxi Medical University, Nanning, Guangxi, China. hexuan269@163.com. AD - Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Guangxi, China. AD - Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi, China. AD - Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi, China. FAU - Ma, Xuemeng AU - Ma X AD - Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Guangxi, China. AD - Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi, China. AD - Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi, China. AD - Department of Oral Pathology, College of Stomatology, Hospital of Stomatology, Guangxi Medical University, Guangxi, China. FAU - Meng, Zijun AU - Meng Z AD - Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Guangxi, China. AD - Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi, China. AD - Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi, China. AD - Department of Operative Dentistry and Endodontology, College of Stomatology, Hospital of Stomatology, Guangxi Medical University, Nanning, Guangxi, China. FAU - Han, Zhiqi AU - Han Z AD - Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Guangxi, China. AD - Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi, China. AD - Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi, China. FAU - Chen, Wenxia AU - Chen W AD - Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Guangxi, China. AD - Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi, China. AD - Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi, China. AD - Department of Operative Dentistry and Endodontology, College of Stomatology, Hospital of Stomatology, Guangxi Medical University, Nanning, Guangxi, China. angelaxiacw@163.com. LA - eng GR - KQGRKF202202/Guangxi Health Commission Key laboratory of prevention and treatment for oral infectious diseases/ GR - 2021009/Nanning Qingxiu District Science and Technology Plan/ GR - GXMUYSF202124/Youth Science Foundation of Guangxi Medical University/ PT - Journal Article DEP - 20221207 PL - Spain TA - Histol Histopathol JT - Histology and histopathology JID - 8609357 RN - 0 (beta Catenin) RN - 136601-57-5 (Cyclin D1) RN - 0 (NF-kappa B) RN - 0 (Cadherins) RN - 0 (DNA, Ribosomal) SB - IM MH - Humans MH - *Carcinoma, Squamous Cell/metabolism MH - Squamous Cell Carcinoma of Head and Neck MH - *Mouth Neoplasms/pathology MH - Fusobacterium nucleatum MH - beta Catenin MH - Cyclin D1 MH - NF-kappa B MH - In Situ Hybridization, Fluorescence MH - Endothelial Cells/pathology MH - *Head and Neck Neoplasms MH - Cadherins MH - DNA, Ribosomal EDAT- 2022/12/09 06:00 MHDA- 2023/07/17 06:42 CRDT- 2022/12/08 12:53 PHST- 2023/07/17 06:42 [medline] PHST- 2022/12/09 06:00 [pubmed] PHST- 2022/12/08 12:53 [entrez] AID - HH-18-560 [pii] AID - 10.14670/HH-18-560 [doi] PST - ppublish SO - Histol Histopathol. 2023 Aug;38(8):929-939. doi: 10.14670/HH-18-560. Epub 2022 Dec 7.