PMID- 36478797
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221210
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 8
IP  - 12
DP  - 2022 Dec
TI  - Ephedra alata subsp. alenda (Ephedraceae) leaf extracts: phytochemical screening, 
      anti-diabetic, anti-obesity and anti-toxic activities on diabetic-induced 
      liver-kidney-testes toxicities and inhibition of alpha-amylase and lipase enzymes.
PG  - e11954
LID - 10.1016/j.heliyon.2022.e11954 [doi]
LID - e11954
AB  - The study evaluated the phytochemical composition of Ephedra alata and its 
      effects on alpha-amylase and lipase enzymes and diabetic-induced liver-kidney-testes 
      toxicities to determine the anti-diabetic, anti-obesity, and anti-toxic 
      potentials of the plant. Obesity was induced by a high-fat and fructose diet 
      (HFFD). Various compounds were identified and quantified: cafeic acid, apigenin 
      7-O-glucoside, apigenin, rutin, luteolin 7-O-glucoside, p-Coumaric acid and 
      others in EA aqueous extract (EAWE). In vitro, this study showed that EAWE 
      strongly inhibited lipase activity as compared to EA methanol (EAME) and ethyl 
      acetate EA extracts (EAEE). In obese rats, the supplementation of EAWE inhibited 
      significantly (P < 0.01) intestinal and pancreatic lipase activity by 35 and 36% 
      respectively. This decrease in lipid digestive enzyme activity caused a 
      significant (P < 0.05) reduce in the weight gain by 12.7% and significant (P < 
      0.05) decrease in the serum lipid rate as total cholesterol (TC), low-density 
      lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C). 
      Moreover, the supplementation of EAWE to obese rats reduced the activity of 
      alpha-amylase in the small intestine and pancreas by 26 and 31% respectively (P < 
      0.01) and consequently decreases in serum glucose level by 20.8% (P < 0.05). In 
      addition, administration of EAWE in type 2 diabetes protected from obesity 
      induced liver, kidney and testes alterations. The potent protective effect EAWE 
      may be influenced by the diversity of phenolic compounds. therefore, this study 
      showed in the first time that EAWE are efficient for the prevention and the 
      amelioration of obesity, hyperglycemia, and various organs toxicities.
CI  - (c) 2022 The Author(s).
FAU - Saidi, Saber Abdelkader
AU  - Saidi SA
AD  - Department of Biology, College of Sciences and Arts-Khulis, University of Jeddah, 
      Jeddah, Saudi Arabia.
FAU - Al-Shaikh, Turki M
AU  - Al-Shaikh TM
AD  - Department of Biology, College of Sciences and Arts-Khulis, University of Jeddah, 
      Jeddah, Saudi Arabia.
FAU - Alghamdi, Othman A
AU  - Alghamdi OA
AD  - Department of Biological Sciences, College of Science, University of Jeddah, 
      Jeddah, Saudi Arabia.
FAU - Hamden, Khaled
AU  - Hamden K
AD  - Laboratory of Bioresources: Integrative Biology and Exploiting, Higher Institute 
      of Biotechnology of Monastir, University of Monastir, Tunisia.
LA  - eng
PT  - Journal Article
DEP - 20221129
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC9720601
OTO - NOTNLM
OT  - Ephedra alata
OT  - Kidney
OT  - Liver
OT  - Obesity
OT  - Testes
OT  - Type 2 diabetes
COIS- The authors declare no conflict of interest.
EDAT- 2022/12/09 06:00
MHDA- 2022/12/09 06:01
PMCR- 2022/11/29
CRDT- 2022/12/08 13:48
PHST- 2022/03/09 00:00 [received]
PHST- 2022/06/14 00:00 [revised]
PHST- 2022/11/21 00:00 [accepted]
PHST- 2022/12/08 13:48 [entrez]
PHST- 2022/12/09 06:00 [pubmed]
PHST- 2022/12/09 06:01 [medline]
PHST- 2022/11/29 00:00 [pmc-release]
AID - S2405-8440(22)03242-X [pii]
AID - e11954 [pii]
AID - 10.1016/j.heliyon.2022.e11954 [doi]
PST - epublish
SO  - Heliyon. 2022 Nov 29;8(12):e11954. doi: 10.1016/j.heliyon.2022.e11954. 
      eCollection 2022 Dec.