PMID- 36479920
OWN - NLM
STAT- MEDLINE
DCOM- 20230202
LR  - 20230203
IS  - 1939-1676 (Electronic)
IS  - 0891-6640 (Print)
IS  - 0891-6640 (Linking)
VI  - 37
IP  - 1
DP  - 2023 Jan
TI  - Protein microarray allergen profiling in bronchoalveolar lavage fluid and serum 
      of horses with asthma.
PG  - 328-337
LID - 10.1111/jvim.16600 [doi]
AB  - BACKGROUND: The diagnostic value of allergen-specific immunoglobulin E (IgE) in 
      horses with asthma is uncertain. A recently developed protein microarray detected 
      abnormally high latex-specific IgE concentrations in the serum of horses with 
      severe asthma. OBJECTIVES: The main objective was to characterize the IgE 
      profiles of asthmatic horses in Switzerland using a protein microarray platform 
      in serum and bronchoalveolar lavage fluid (BALF). The secondary objective was to 
      determine whether serological and BALF allergen-specific IgE concentrations 
      correlated. ANIMALS: Forty-four asthmatic and 39 control horses >/=5 years of age. 
      METHODS: This prospective cross-sectional study investigated the sensitization 
      profiles of horses with asthma compared with environmentally matched healthy 
      controls. Both serum and BALF were analyzed using the protein microarray. Partial 
      least square-discriminant analysis (PLS-DA) was used to identify and rank the 
      importance of the allergens for class detection (ie, asthma vs control), with a 
      variable influence on the projection (VIP) >1 considered significant. RESULTS: 
      The allergens that best discriminated (VIP >1) asthmatic horses from controls 
      were proteins derived from fungi (Aspergillus fumigatus), insects (Culicoides 
      spp.), and latex (Hevea brasiliensis). The serological model predictive ability 
      was markedly inferior (area under the curve [AUC] 0.585, 95% confidence interval 
      [CI]: 0.454-0.747) to that of the BALF (AUC 0.751, 95% CI: 0.582-0.866). The two 
      models shared nine allergens, of which eight showed significant weak to moderate 
      correlations. CONCLUSION AND CLINICAL IMPORTANCE: The concentrations of several 
      allergen-specific IgE were higher in asthmatic horses. The protein microarray 
      performed better on BALF than serum for detection of asthma. Serological IgE 
      concentrations do not closely correlate with BALF concentrations and should be 
      interpreted with caution.
CI  - (c) 2022 The Authors. Journal of Veterinary Internal Medicine published by Wiley 
      Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
FAU - Wyler, Michelle
AU  - Wyler M
AUID- ORCID: 0000-0002-3441-6587
AD  - Swiss Institute of Equine Medicine (ISME), Department of Clinical Veterinary 
      Medicine, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
FAU - Sage, Sophie Elena
AU  - Sage SE
AUID- ORCID: 0000-0002-6945-070X
AD  - Swiss Institute of Equine Medicine (ISME), Department of Clinical Veterinary 
      Medicine, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
FAU - Marti, Eliane
AU  - Marti E
AD  - Department of Clinical Research and Veterinary Public Health, University of Bern, 
      Bern, Switzerland.
FAU - White, Samuel
AU  - White S
AUID- ORCID: 0000-0002-3675-7545
AD  - Department of Animal, Equine and Veterinary Sciences, Nottingham Trent 
      University, Nottinghamshire, UK.
FAU - Gerber, Vinzenz
AU  - Gerber V
AD  - Swiss Institute of Equine Medicine (ISME), Department of Clinical Veterinary 
      Medicine, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
LA  - eng
GR  - 33-890/Institut Suisse de Medecine Equine/
PT  - Journal Article
DEP - 20221208
PL  - United States
TA  - J Vet Intern Med
JT  - Journal of veterinary internal medicine
JID - 8708660
RN  - 0 (Allergens)
RN  - 0 (Latex)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Horses
MH  - Allergens
MH  - Protein Array Analysis/veterinary
MH  - Bronchoalveolar Lavage Fluid
MH  - Prospective Studies
MH  - Cross-Sectional Studies
MH  - Latex
MH  - *Asthma/veterinary
MH  - Immunoglobulin E
MH  - *Horse Diseases
PMC - PMC9889601
OTO - NOTNLM
OT  - IgE
OT  - allergy
OT  - fungi
OT  - horse
OT  - latex
COIS- Authors declare no conflict of interest.
EDAT- 2022/12/09 06:00
MHDA- 2023/02/03 06:00
PMCR- 2022/12/08
CRDT- 2022/12/08 14:32
PHST- 2021/12/29 00:00 [received]
PHST- 2022/11/16 00:00 [accepted]
PHST- 2022/12/09 06:00 [pubmed]
PHST- 2023/02/03 06:00 [medline]
PHST- 2022/12/08 14:32 [entrez]
PHST- 2022/12/08 00:00 [pmc-release]
AID - JVIM16600 [pii]
AID - 10.1111/jvim.16600 [doi]
PST - ppublish
SO  - J Vet Intern Med. 2023 Jan;37(1):328-337. doi: 10.1111/jvim.16600. Epub 2022 Dec 
      8.