PMID- 36480866
OWN - NLM
STAT- MEDLINE
DCOM- 20221215
LR  - 20221215
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 102
IP  - 45
DP  - 2022 Dec 6
TI  - [Potential application of improved hard tissue section technique in the clinical 
      pathological diagnosis of bone and bone marrow].
PG  - 3617-3623
LID - 10.3760/cma.j.cn112137-20220408-00756 [doi]
AB  - Objective: To discuss the application value of hard tissue section in the 
      clinicopathology diagnosis. Methods: From March 2021 to December 2021, bone 
      slices of 19 patients (1 patient with osteochondroma, 2 patients with 
      chondrosarcoma, 4 patients with osteosarcoma, 2 patients with fibrous dysplasia, 
      2 patients with bone metastasis from thyroid papillary carcinoma, 2 patients with 
      osteomyelitis, 4 patients with giant cell tumor of bone, 2 patients with Ewing 
      sarcoma) and 16 hemopathy patients were collected from the Department of 
      Pathology, Shanghai Sixth People's Hospital. Of the osteopathy patients, there 
      were 14 male and 5 female with a median age of 31 (10-66) years. Meanwhile, there 
      were 7 male and 9 female with a median age of 28 (16-65) years among these 
      hemopathy patients. Thirty-five cases were treated with modified hard tissue 
      slicing technique and paraffin embedding technique, respectively. The advantages 
      and disadvantages of the two methods for clinical diagnosis of bone disease were 
      compared by Hematoxylin-Eosin staining (H&E staining), immunohistochemical 
      staining (IHC), fluorescence in situ hybridization (FISH) and Sanger sequencing. 
      Results: The improved resin-embedded method showed better histological morphology 
      and cell structure. Besides, the expression of Ki67, SATB2, CD34, SMA, 
      CD68,MPO,CD4 and CD33 in immunohistochemical staining in bone tissues which were 
      embedded in resin were more clear in the accurate positive localization than 
      those using paraffin-embedded. MDM2 of FISH exhibited a higher fluorescence 
      intensity and more accurate location. Meanwhile, both methods treated with Sanger 
      sequencing met the requirements of DNA purity and mutation detection. Conclusion: 
      The improved hard tissue section method is simple and short time-consuming, which 
      is suitable for optimizing the clinical bone and bone marrow pathological 
      diagnosis process.
FAU - Yin, J H
AU  - Yin JH
AD  - Department of Orthopaedic Surgery, Shanghai Sixth People's Hospital, Shanghai 
      200233, China.
FAU - Zhou, J
AU  - Zhou J
AD  - Department of Pathology, Shanghai Sixth People's Hospital, Shanghai 200233, 
      China.
FAU - Chen, Y W
AU  - Chen YW
AD  - Department of Orthopaedic Surgery, Shanghai Sixth People's Hospital, Shanghai 
      200233, China.
FAU - Li, H P
AU  - Li HP
AD  - Department of Hematology, Shanghai Sixth People's Hospital, Shanghai 200233, 
      China.
FAU - Tao, Y
AU  - Tao Y
AD  - Department of Hematology, Shanghai Sixth People's Hospital, Shanghai 200233, 
      China.
FAU - Chang, C K
AU  - Chang CK
AD  - Department of Hematology, Shanghai Sixth People's Hospital, Shanghai 200233, 
      China.
FAU - Zhang, C Q
AU  - Zhang CQ
AD  - Department of Orthopaedic Surgery, Shanghai Sixth People's Hospital, Shanghai 
      200233, China.
FAU - Liu, Zhiyan
AU  - Liu Z
AD  - Department of Pathology, Shanghai Sixth People's Hospital, Shanghai 200233, 
      China.
LA  - chi
GR  - 81802247/National Natural Science Foundation of China/
GR  - 2018YFC1106300/National Key Research and Development Project of China/
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
SB  - IM
MH  - Female
MH  - Male
MH  - Animals
MH  - *Bone Marrow
MH  - In Situ Hybridization, Fluorescence
MH  - China
MH  - *Bone and Bones
EDAT- 2022/12/09 06:00
MHDA- 2022/12/15 06:00
CRDT- 2022/12/08 22:13
PHST- 2022/12/08 22:13 [entrez]
PHST- 2022/12/09 06:00 [pubmed]
PHST- 2022/12/15 06:00 [medline]
AID - 10.3760/cma.j.cn112137-20220408-00756 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2022 Dec 6;102(45):3617-3623. doi: 
      10.3760/cma.j.cn112137-20220408-00756.