PMID- 36481484
OWN - NLM
STAT- MEDLINE
DCOM- 20221221
LR  - 20230217
IS  - 1879-260X (Electronic)
IS  - 0925-4439 (Linking)
VI  - 1869
IP  - 2
DP  - 2023 Feb
TI  - PSMA2 knockdown impacts expression of proteins involved in immune and cellular 
      stress responses in human lung cells.
PG  - 166617
LID - S0925-4439(22)00288-5 [pii]
LID - 10.1016/j.bbadis.2022.166617 [doi]
AB  - Proteasome subunit alpha type-2 (PSMA2) is a critical component of the 20S 
      proteasome, which is the core particle of the 26S proteasome complex and is 
      involved in cellular protein quality control by recognizing and recycling 
      defective proteins. PSMA2 expression dysregulation has been detected in different 
      human diseases and viral infections. No study yet has reported PSMA2 knockdown 
      (KD) effects on the cellular proteome. METHODS: We used SOMAScan, an 
      aptamer-based multiplexed technique, to measure >1300 human proteins to determine 
      the impact of PSMA2 KD on A549 human lung epithelial cells. RESULTS: PSMA2 KD 
      resulted in significant dysregulation of 52 cellular proteins involved in 
      different bio-functions, including cellular movement and development, cell death 
      and survival, and cancer. The immune system and signal transduction were the most 
      affected cellular functions. PSMA2 KD caused dysregulation of several signaling 
      pathways involved in immune response, cytokine signaling, organismal growth and 
      development, cellular stress and injury (including autophagy and unfolded protein 
      response), and cancer responses. CONCLUSIONS: In summary, this study helps us 
      better understand the importance of PSMA2 in different cellular functions, 
      signaling pathways, and human diseases.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Rashid, Mahamud-Ur
AU  - Rashid MU
AD  - University of Manitoba, Department of Medical Microbiology & Infectious Diseases, 
      Room 543 Basic Medical Sciences Building, 745 Bannatyne Ave., Winnipeg, MB R3E 
      0J9, Canada; Manitoba Centre for Proteomics & Systems Biology, Room 799, 715 
      McDermot Ave., Winnipeg, MB R3E 3P4, Canada.
FAU - Lorzadeh, Shahrokh
AU  - Lorzadeh S
AD  - Department of Human Anatomy and Cell Science, Max Rady College of Medicine, 
      University of Manitoba, Winnipeg, MB R3E 0V9, Canada.
FAU - Gao, Ang
AU  - Gao A
AD  - Manitoba Centre for Proteomics & Systems Biology, Room 799, 715 McDermot Ave., 
      Winnipeg, MB R3E 3P4, Canada.
FAU - Ghavami, Saeid
AU  - Ghavami S
AD  - Department of Human Anatomy and Cell Science, Max Rady College of Medicine, 
      University of Manitoba, Winnipeg, MB R3E 0V9, Canada; Research Institutes of 
      Oncology and Hematology, Cancer Care Manitoba-University of Manitoba, Winnipeg, 
      MB R3E 0V9, Canada; Biology of Breathing Theme, Children Hospital Research 
      Institute of Manitoba, University of Manitoba, Winnipeg, MB R3E 0V9, Canada.
FAU - Coombs, Kevin M
AU  - Coombs KM
AD  - University of Manitoba, Department of Medical Microbiology & Infectious Diseases, 
      Room 543 Basic Medical Sciences Building, 745 Bannatyne Ave., Winnipeg, MB R3E 
      0J9, Canada; Manitoba Centre for Proteomics & Systems Biology, Room 799, 715 
      McDermot Ave., Winnipeg, MB R3E 3P4, Canada; Children's Hospital Research 
      Institute of Manitoba, Room 513, 715 McDermot Ave., Winnipeg, MB R3E 3P4, Canada. 
      Electronic address: kevin.coombs@umanitoba.ca.
LA  - eng
GR  - MOP-106713/CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221205
PL  - Netherlands
TA  - Biochim Biophys Acta Mol Basis Dis
JT  - Biochimica et biophysica acta. Molecular basis of disease
JID - 101731730
RN  - 0 (Proteome)
SB  - IM
MH  - Humans
MH  - *Unfolded Protein Response
MH  - *Proteome/metabolism
MH  - Signal Transduction
MH  - Lung/metabolism
OTO - NOTNLM
OT  - Autophagy, endoplasmic reticulum stress
OT  - Immune system
OT  - PSMA2
OT  - Proteasome
OT  - Signal transduction
COIS- Declaration of competing interest Kevin M. Coombs reports financial support was 
      provided by Canadian Institutes of Health Research. Mahamud ur-Rashid reports 
      financial support was provided by Research Manitoba. Saeid Ghavami reports 
      financial support was provided by Research Manitoba.
EDAT- 2022/12/09 06:00
MHDA- 2022/12/22 06:00
CRDT- 2022/12/08 23:35
PHST- 2022/07/12 00:00 [received]
PHST- 2022/11/21 00:00 [revised]
PHST- 2022/11/28 00:00 [accepted]
PHST- 2022/12/09 06:00 [pubmed]
PHST- 2022/12/22 06:00 [medline]
PHST- 2022/12/08 23:35 [entrez]
AID - S0925-4439(22)00288-5 [pii]
AID - 10.1016/j.bbadis.2022.166617 [doi]
PST - ppublish
SO  - Biochim Biophys Acta Mol Basis Dis. 2023 Feb;1869(2):166617. doi: 
      10.1016/j.bbadis.2022.166617. Epub 2022 Dec 5.