PMID- 36482019
OWN - NLM
STAT- MEDLINE
DCOM- 20230420
LR  - 20230425
IS  - 1573-675X (Electronic)
IS  - 1360-8185 (Linking)
VI  - 28
IP  - 3-4
DP  - 2023 Apr
TI  - Long non-coding RNA SNHG4 enhances RNF14 mRNA stability to promote the 
      progression of colorectal cancer by recruiting TAF15 protein.
PG  - 414-431
LID - 10.1007/s10495-022-01781-6 [doi]
AB  - SNHG4 is a lncRNA that was previously reported to promote colorectal cancer (CRC) 
      progression via molecular sponge mechanism. Bioinformatic analysis suggested 
      SNHG4 might scaffold TAF15 protein-RNF14 mRNA interaction. We aimed to 
      investigate the mechanisms of potential SNHG4/TAF15/RNF14 axis in promoting CRC 
      malignant phenotypes. Protein-RNA interaction was determined using RNA 
      immunoprecipitation, pull-down and fluorescence in situ hybridization (FISH) 
      combined immunofluorescence assays. Cell apoptosis rates were quantified using 
      flow cytometry. CCK-8 and colony formation were adopted to determine cell 
      proliferation. Wound healing and transwell assays were employed to assess cell 
      migration and invasion, respectively. Xenograft tumor model was applied to assess 
      the effects of SNHG4 on CRC tumorigenesis in vivo. SNHG4, TAF15 and RNF14 were 
      up-regulated in CRC tissues. SNHG4 overexpression promoted cell proliferation, 
      migration, invasion, and Wnt/beta-catenin pathway activation in vitro, as well as 
      tumor growth in vivo. The inhibited malignant phenotypes caused by SNHG4 
      knockdown were impeded by TAF15 or RNF14 overexpression. Mechanistically, SNHG4 
      recruited TAF15 protein and thus promoted the interaction between TAF15 protein 
      and RNF14 mRNA, leading to the increased RNF14 mRNA stability. This in turn 
      facilitated the Wnt/beta-catenin signal transduction. SNHG4 enhanced RNF14 mRNA 
      stability and activated the Wnt/beta-catenin pathway to promote the progression of 
      colorectal cancer by recruiting TAF15 protein.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Lv, Lv
AU  - Lv L
AD  - Department of Breast and Thyroid Surgery, Liuzhou People's Hospital, NO.8, 
      Wenchang Road, Liuzhou, 545006, Guangxi, People's Republic of China. 
      lvlv1984@glmc.edu.cn.
FAU - Huang, Bojie
AU  - Huang B
AD  - Department of Gastrointestinal Surgery, Affiliated Hospital of Guilin Medical 
      University, Guilin, 541001, Guangxi, People's Republic of China.
FAU - Yi, Lu
AU  - Yi L
AD  - Department of Dermatology & Venerology, Affiliated Hospital of Guilin Medical 
      University, Guilin, 541001, Guangxi, People's Republic of China.
FAU - Zhang, Li
AU  - Zhang L
AD  - Department of Breast and Thyroid Surgery, Liuzhou People's Hospital, NO.8, 
      Wenchang Road, Liuzhou, 545006, Guangxi, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221209
PL  - Netherlands
TA  - Apoptosis
JT  - Apoptosis : an international journal on programmed cell death
JID - 9712129
RN  - 0 (beta Catenin)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Messenger)
RN  - 0 (TAF15 protein, human)
RN  - 0 (TATA-Binding Protein Associated Factors)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Apoptosis/genetics
MH  - beta Catenin/metabolism
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - *Colorectal Neoplasms/pathology
MH  - Disease Models, Animal
MH  - Gene Expression Regulation, Neoplastic
MH  - In Situ Hybridization, Fluorescence
MH  - *MicroRNAs/genetics
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - RNA, Messenger
MH  - *TATA-Binding Protein Associated Factors/genetics/metabolism
MH  - Wnt Signaling Pathway
MH  - Intracellular Signaling Peptides and Proteins/metabolism
OTO - NOTNLM
OT  - Colorectal cancer
OT  - RNF14
OT  - SNHG4
OT  - TAF15
OT  - Wnt/beta-catenin pathway
EDAT- 2022/12/10 06:00
MHDA- 2023/04/20 10:17
CRDT- 2022/12/09 00:28
PHST- 2022/10/14 00:00 [accepted]
PHST- 2023/04/20 10:17 [medline]
PHST- 2022/12/10 06:00 [pubmed]
PHST- 2022/12/09 00:28 [entrez]
AID - 10.1007/s10495-022-01781-6 [pii]
AID - 10.1007/s10495-022-01781-6 [doi]
PST - ppublish
SO  - Apoptosis. 2023 Apr;28(3-4):414-431. doi: 10.1007/s10495-022-01781-6. Epub 2022 
      Dec 9.