PMID- 36483968
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221210
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 13
DP  - 2022
TI  - Oat chromosome and genome evolution defined by widespread terminal intergenomic 
      translocations in polyploids.
PG  - 1026364
LID - 10.3389/fpls.2022.1026364 [doi]
LID - 1026364
AB  - Structural chromosome rearrangements involving translocations, fusions and 
      fissions lead to evolutionary variation between species and potentially 
      reproductive isolation and variation in gene expression. While the wheats 
      (Triticeae, Poaceae) and oats (Aveneae) all maintain a basic chromosome number of 
      x=7, genomes of oats show frequent intergenomic translocations, in contrast to 
      wheats where these translocations are relatively rare. We aimed to show genome 
      structural diversity and genome relationships in tetraploid, hexaploid and 
      octoploid Avena species and amphiploids, establishing patterns of intergenomic 
      translocations across different oat taxa using fluorescence in situ hybridization 
      (FISH) with four well-characterized repetitive DNA sequences: pAs120, AF226603, 
      Ast-R171 and Ast-T116. In A. agadiriana (2n=4x=28), the selected probes 
      hybridized to all chromosomes indicating that this species originated from one 
      (autotetraploid) or closely related ancestors with the same genomes. Hexaploid 
      amphiploids were confirmed as having the genomic composition AACCDD, while 
      octoploid amphiploids showed three different genome compositions: AACCCCDD, 
      AAAACCDD or AABBCCDD. The A, B, C, and D genomes of oats differ significantly in 
      their involvement in non-centromeric, intercalary translocations. There was a 
      predominance of distal intergenomic translocations from the C- into the D-genome 
      chromosomes. Translocations from A- to C-, or D- to C-genome chromosomes were 
      less frequent, proving that at least some of the translocations in oat polyploids 
      are non-reciprocal. Rare translocations from A- to D-, D- to A- and C- to 
      B-genome chromosomes were also visualized. The fundamental research has 
      implications for exploiting genomic biodiversity in oat breeding through 
      introgression from wild species potentially with contrasting chromosomal 
      structures and hence deleterious segmental duplications or large deletions in 
      amphiploid parental lines.
CI  - Copyright (c) 2022 Tomaszewska, Schwarzacher and Heslop-Harrison.
FAU - Tomaszewska, Paulina
AU  - Tomaszewska P
AD  - Department of Genetics and Genome Biology, University of Leicester, Leicester, 
      United Kingdom.
AD  - Department of Genetics and Cell Physiology, Faculty of Biological Sciences, 
      University of Wroclaw, Wroclaw, Poland.
FAU - Schwarzacher, Trude
AU  - Schwarzacher T
AD  - Department of Genetics and Genome Biology, University of Leicester, Leicester, 
      United Kingdom.
AD  - Key Laboratory of Plant Resources Conservation and Sustainable 
      Utilization/Guangdong Provincial, Key Laboratory of Applied Botany, South China 
      Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
FAU - Heslop-Harrison, J S Pat
AU  - Heslop-Harrison JSP
AD  - Department of Genetics and Genome Biology, University of Leicester, Leicester, 
      United Kingdom.
AD  - Key Laboratory of Plant Resources Conservation and Sustainable 
      Utilization/Guangdong Provincial, Key Laboratory of Applied Botany, South China 
      Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20221122
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC9725029
OTO - NOTNLM
OT  - Avena
OT  - amphiploids
OT  - chromosome rearrangements
OT  - genome identification
OT  - intergenomic translocations
OT  - polyploidy
OT  - repetitive DNA motifs
OT  - structural variation
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/12/10 06:00
MHDA- 2022/12/10 06:01
PMCR- 2022/01/01
CRDT- 2022/12/09 04:15
PHST- 2022/08/23 00:00 [received]
PHST- 2022/10/14 00:00 [accepted]
PHST- 2022/12/09 04:15 [entrez]
PHST- 2022/12/10 06:00 [pubmed]
PHST- 2022/12/10 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2022.1026364 [doi]
PST - epublish
SO  - Front Plant Sci. 2022 Nov 22;13:1026364. doi: 10.3389/fpls.2022.1026364. 
      eCollection 2022.