PMID- 36484201
OWN - NLM
STAT- MEDLINE
DCOM- 20230227
LR  - 20230227
IS  - 0261-1929 (Print)
IS  - 0261-1929 (Linking)
VI  - 51
IP  - 1
DP  - 2023 Jan
TI  - Cardiac Transcription Regulators Differentiate Human Umbilical Cord Mesenchymal 
      Stem Cells into Cardiac Cells.
PG  - 12-29
LID - 10.1177/02611929221143774 [doi]
AB  - Stem cell-based therapy presents an attractive alternative to conventional 
      therapies for degenerative diseases. Numerous studies have investigated the 
      capability of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) to 
      contribute to the regeneration of cardiomyocytes, and the results have encouraged 
      further basic and clinical studies on the MSC-based treatment of 
      cardiomyopathies. This study aimed to determine the potential of cardiomyogenic 
      transcription factors in differentiating hUC-MSCs into cardiac-like cells in 
      vitro. MSCs were isolated from umbilical cord tissue and were transduced with the 
      transcription factor genes, GATA-4 and Nkx 2.5, via infection with lentiviruses, 
      to promote differentiation into the cardiomyogenic lineage. Gene and protein 
      expression were analysed with qPCR and immunocytochemical staining. After 
      transduction, differentiated cardiac-like cells showed significant expression of 
      cardiac genes and proteins, namely GATA-4, Nkx-2.5, cardiac troponin I (cTnI) and 
      myosin heavy chain (MHC). The cardiomyogenic-induced group significantly 
      overexpressed cardiac-specific genes (GATA-4, Nkx-2.5, cTnI, MHC, alpha-actinin and 
      Wnt2). Expression of the calcium channel gene was also significantly increased, 
      while the sodium channel gene was downregulated in the transduced hUC-MSCs, as 
      compared to non-transduced cells. The results suggest that GATA-4 and Nkx-2.5 
      interact synergistically in the activation of downstream cardiac transcription 
      factors, demonstrating the functional convergence of hUC-MSC differentiation into 
      cardiac-like cells. These findings could potentially be utilised in the efficient 
      production of cardiac-like cells from stem cells; these cardiac-like cells could 
      then be used in various applications, such as for in vivo implantation in 
      infarcted myocardium, and for drug screening in toxicity testing.
FAU - Shafique, Shumaila
AU  - Shafique S
AD  - 208246Dr Panjwani Center for Molecular Medicine and Drug Research, International 
      Center for Chemical and Biological Sciences, University of Karachi, Karachi, 
      Pakistan.
FAU - Ali, Syeda Roohina
AU  - Ali SR
AD  - 208246Dr Panjwani Center for Molecular Medicine and Drug Research, International 
      Center for Chemical and Biological Sciences, University of Karachi, Karachi, 
      Pakistan.
FAU - Rajput, Shafiqa Naeem
AU  - Rajput SN
AD  - 208246Dr Panjwani Center for Molecular Medicine and Drug Research, International 
      Center for Chemical and Biological Sciences, University of Karachi, Karachi, 
      Pakistan.
FAU - Salim, Asmat
AU  - Salim A
AD  - 208246Dr Panjwani Center for Molecular Medicine and Drug Research, International 
      Center for Chemical and Biological Sciences, University of Karachi, Karachi, 
      Pakistan.
FAU - Khan, Irfan
AU  - Khan I
AUID- ORCID: 0000-0003-1878-7836
AD  - 208246Dr Panjwani Center for Molecular Medicine and Drug Research, International 
      Center for Chemical and Biological Sciences, University of Karachi, Karachi, 
      Pakistan.
LA  - eng
PT  - Journal Article
DEP - 20221209
PL  - England
TA  - Altern Lab Anim
JT  - Alternatives to laboratory animals : ATLA
JID - 8110074
RN  - 0 (Transcription Factors)
RN  - 0 (Troponin I)
SB  - IM
MH  - Humans
MH  - Cell Differentiation/physiology
MH  - *Myocardium/metabolism
MH  - Transcription Factors/metabolism
MH  - Troponin I/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Umbilical Cord/metabolism
OTO - NOTNLM
OT  - cardiac
OT  - differentiation
OT  - gene expression
OT  - immunostaining
OT  - transduction
OT  - umbilical cord mesenchymal stem cells
EDAT- 2022/12/10 06:00
MHDA- 2023/03/03 06:00
CRDT- 2022/12/09 04:38
PHST- 2022/12/10 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2022/12/09 04:38 [entrez]
AID - 10.1177/02611929221143774 [doi]
PST - ppublish
SO  - Altern Lab Anim. 2023 Jan;51(1):12-29. doi: 10.1177/02611929221143774. Epub 2022 
      Dec 9.