PMID- 36494916
OWN - NLM
STAT- MEDLINE
DCOM- 20230405
LR  - 20230503
IS  - 1476-5381 (Electronic)
IS  - 0007-1188 (Linking)
VI  - 180
IP  - 9
DP  - 2023 May
TI  - Non-neuronal TRPA1 encodes mechanical allodynia associated with neurogenic 
      inflammation and partial nerve injury in rats.
PG  - 1232-1246
LID - 10.1111/bph.16005 [doi]
AB  - BACKGROUND AND PURPOSE: The pro-algesic transient receptor potential ankyrin 1 
      (TRPA1) channel, expressed by a subpopulation of primary sensory neurons, has 
      been implicated in various pain models in mice. However, evidence in rats 
      indicates that TRPA1 conveys nociceptive signals elicited by channel activators, 
      but not those associated with tissue inflammation or nerve injury. Here, in rats, 
      we explored the TRPA1 role in mechanical allodynia associated with stimulation of 
      peptidergic primary sensory neurons (neurogenic inflammation) and moderate 
      (partial sciatic nerve ligation, pSNL) or severe (chronic constriction injury, 
      CCI) sciatic nerve injury. EXPERIMENTAL APPROACH: Acute nociception and 
      mechanical hypersensitivity associated with neurogenic inflammation and sciatic 
      nerve injury (pSNL and CCI) were investigated in rats with TRPA1 pharmacological 
      antagonism or genetic silencing. TRPA1 presence and function were analysed in 
      cultured rat Schwann cells. KEY RESULTS: Hind paw mechanical allodynia (HPMA), 
      but not acute nociception, evoked by local injection of capsaicin or allyl 
      isothiocyanate, the TRP vanilloid 1 (TRPV1) or the TRPA1 activators was mediated 
      by CGRP released from peripheral sensory nerve terminals. CGRP-evoked HPMA was 
      sustained by a ROS-dependent TRPA1 activation, probably in Schwann cells. HPMA 
      evoked by pSNL, but not that evoked by CCI, was mediated by ROS and TRPA1 without 
      the involvement of CGRP. CONCLUSIONS AND IMPLICATIONS: As found in mice, TRPA1 
      mediates mechanical allodynia associated with neurogenic inflammation and 
      moderate nerve injury in rats. The channel contribution to mechanical 
      hypersensitivity is a common feature in rodents and might be explored in humans.
CI  - (c) 2022 The Authors. British Journal of Pharmacology published by John Wiley & 
      Sons Ltd on behalf of British Pharmacological Society.
FAU - De Logu, Francesco
AU  - De Logu F
AUID- ORCID: 0000-0001-8360-6929
AD  - Department of Health Sciences, Clinical Pharmacology and Oncology Section, 
      University of Florence, Florence, Italy.
FAU - De Siena, Gaetano
AU  - De Siena G
AD  - Department of Health Sciences, Clinical Pharmacology and Oncology Section, 
      University of Florence, Florence, Italy.
FAU - Landini, Lorenzo
AU  - Landini L
AD  - Department of Health Sciences, Clinical Pharmacology and Oncology Section, 
      University of Florence, Florence, Italy.
FAU - Marini, Matilde
AU  - Marini M
AD  - Department of Health Sciences, Clinical Pharmacology and Oncology Section, 
      University of Florence, Florence, Italy.
FAU - Souza Monteiro de Araujo, Daniel
AU  - Souza Monteiro de Araujo D
AD  - Department of Health Sciences, Clinical Pharmacology and Oncology Section, 
      University of Florence, Florence, Italy.
FAU - Albanese, Valentina
AU  - Albanese V
AD  - Department of Environmental and Prevention Sciences - DEPS, University of 
      Ferrara, Ferrara, Italy.
FAU - Preti, Delia
AU  - Preti D
AD  - Department of Chemical, Pharmaceutical and Agricultural Sciences, University of 
      Ferrara, Ferrara, Italy.
FAU - Romitelli, Antonia
AU  - Romitelli A
AUID- ORCID: 0000-0003-2495-8769
AD  - Department of Health Sciences, Clinical Pharmacology and Oncology Section, 
      University of Florence, Florence, Italy.
FAU - Chieca, Martina
AU  - Chieca M
AD  - Department of Health Sciences, Clinical Pharmacology and Oncology Section, 
      University of Florence, Florence, Italy.
FAU - Titiz, Mustafa
AU  - Titiz M
AD  - Department of Health Sciences, Clinical Pharmacology and Oncology Section, 
      University of Florence, Florence, Italy.
FAU - Iannone, Luigi F
AU  - Iannone LF
AD  - Department of Health Sciences, Clinical Pharmacology and Oncology Section, 
      University of Florence, Florence, Italy.
FAU - Geppetti, Pierangelo
AU  - Geppetti P
AUID- ORCID: 0000-0003-3797-8371
AD  - Department of Health Sciences, Clinical Pharmacology and Oncology Section, 
      University of Florence, Florence, Italy.
FAU - Nassini, Romina
AU  - Nassini R
AUID- ORCID: 0000-0002-9223-8395
AD  - Department of Health Sciences, Clinical Pharmacology and Oncology Section, 
      University of Florence, Florence, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230107
PL  - England
TA  - Br J Pharmacol
JT  - British journal of pharmacology
JID - 7502536
RN  - 0 (TRPA1 Cation Channel)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
RN  - UKW89XAX2X (hydroxypropyl methacrylate)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Transient Receptor Potential Channels)
RN  - 0 (TRPA1 protein, human)
RN  - 0 (Trpa1 protein, mouse)
RN  - 0 (Trpa1 protein, rat)
SB  - IM
MH  - Humans
MH  - Rats
MH  - Mice
MH  - Animals
MH  - Hyperalgesia
MH  - TRPA1 Cation Channel
MH  - Neurogenic Inflammation
MH  - Calcitonin Gene-Related Peptide/metabolism
MH  - Reactive Oxygen Species
MH  - *Transient Receptor Potential Channels
MH  - *Peripheral Nervous System Diseases
OTO - NOTNLM
OT  - Schwann cells
OT  - TRPA1
OT  - nerve injury
OT  - neurogenic inflammation
OT  - oxidative stress
EDAT- 2022/12/11 06:00
MHDA- 2023/04/05 06:42
CRDT- 2022/12/10 01:03
PHST- 2022/11/11 00:00 [revised]
PHST- 2022/07/30 00:00 [received]
PHST- 2022/12/03 00:00 [accepted]
PHST- 2023/04/05 06:42 [medline]
PHST- 2022/12/11 06:00 [pubmed]
PHST- 2022/12/10 01:03 [entrez]
AID - 10.1111/bph.16005 [doi]
PST - ppublish
SO  - Br J Pharmacol. 2023 May;180(9):1232-1246. doi: 10.1111/bph.16005. Epub 2023 Jan 
      7.