PMID- 36499636 OWN - NLM STAT- MEDLINE DCOM- 20221216 LR - 20231103 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 23 IP - 23 DP - 2022 Dec 4 TI - Enhancing the Expression of the OsF3H Gene in Oryza sativa Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance. LID - 10.3390/ijms232315308 [doi] LID - 15308 AB - The white-backed planthopper (WBPH) is a major pest of rice crops and causes severe loss of yield. We previously developed the WBPH-resistant rice cultivar "OxF3H" by overexpressing the OsF3H gene. Although there was a higher accumulation of the flavonoids kaempferol (Kr) and quercetin (Qu) as well as salicylic acid (SA) in OxF3H transgenic (OsF3H or Trans) plants compared to the wild type (WT), it is still unclear how OsF3H overexpression affects these WBPH resistant-related changes in gene expression in OxF3H plants. In this study, we analyze RNA-seq data from OxF3H and WT at several points (0 h, 3 h, 12 h, and 24 h) after WBPH infection to explain how overall changes in gene expression happen in these two cultivars. RT-qPCR further validated a number of the genes. Results revealed that the highest number of DEGs (4735) between the two genotypes was detected after 24 h of infection. Interestingly, it was found that several of the DEGs between the WT and OsF3H under control conditions were also differentially expressed in OsF3H in response to WBPH infestation. These results indicate that significant differences in gene expression between the "OxF3H" and "WT" exist as the infection time increases. Many of these DEGs were related to oxidoreductase activity, response to stress, salicylic acid biosynthesis, metabolic process, defense response to pathogen, cellular response to toxic substance, and regulation of hormone levels. Moreover, genes involved in salicylic acid (SA) and ethylene (Et) biosynthesis were upregulated in OxF3H plants, while jasmonic acid (JA), brassinosteroid (Br), and abscisic acid (ABA) signaling pathways were found downregulated in OxF3H plants during WBPH infestation. Interestingly, many DEGs related to pathogenesis, such as OsPR1, OsPR1b, OsNPR1, OsNPR3, and OsNPR5, were found to be significantly upregulated in OxF3H plants. Additionally, genes related to the MAPKs pathway and about 30 WRKY genes involved in different pathways were upregulated in OxF3H plants after WBPH infestation. This suggests that overexpression of the OxF3H gene leads to multiple transcriptomic changes and impacts plant hormones and pathogenic-related and secondary-metabolites-related genes, enhancing the plant's resistance to WBPH infestation. FAU - Jan, Rahmatullah AU - Jan R AUID- ORCID: 0000-0001-5788-3017 AD - Department of Applied Biosciences, Graduate School, Kyungpook National University, Daegu 41566, Republic of Korea. AD - Coastal Agriculture Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea. FAU - Asaf, Sajjad AU - Asaf S AUID- ORCID: 0000-0001-8216-7549 AD - Natural and Medical Science Research Center, University of Nizwa, Nizwa 611, Oman. FAU - Lubna AU - Lubna AD - Department of Botany, Garden Campus, Abdul Wali Khan University, Mardan 23200, Pakistan. FAU - Asif, Saleem AU - Asif S AUID- ORCID: 0000-0002-2416-7388 AD - Department of Applied Biosciences, Graduate School, Kyungpook National University, Daegu 41566, Republic of Korea. FAU - Kim, Eun-Gyeong AU - Kim EG AUID- ORCID: 0000-0002-8532-608X AD - Department of Applied Biosciences, Graduate School, Kyungpook National University, Daegu 41566, Republic of Korea. FAU - Jang, Yoon-Hee AU - Jang YH AUID- ORCID: 0000-0002-6282-9557 AD - Department of Applied Biosciences, Graduate School, Kyungpook National University, Daegu 41566, Republic of Korea. FAU - Kim, Nari AU - Kim N AD - Department of Applied Biosciences, Graduate School, Kyungpook National University, Daegu 41566, Republic of Korea. FAU - Al-Harrasi, Ahmed AU - Al-Harrasi A AUID- ORCID: 0000-0002-0815-5942 AD - Natural and Medical Science Research Center, University of Nizwa, Nizwa 611, Oman. FAU - Lee, Gang-Seob AU - Lee GS AUID- ORCID: 0000-0001-7470-798X AD - Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic of Korea. FAU - Kim, Kyung-Min AU - Kim KM AUID- ORCID: 0000-0003-4812-6297 AD - Department of Applied Biosciences, Graduate School, Kyungpook National University, Daegu 41566, Republic of Korea. AD - Coastal Agriculture Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea. LA - eng PT - Journal Article DEP - 20221204 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - O414PZ4LPZ (Salicylic Acid) SB - IM MH - Animals MH - *Oryza/metabolism MH - Biosynthetic Pathways MH - Plant Diseases/genetics MH - Salicylic Acid/metabolism MH - Plants, Genetically Modified/metabolism MH - Insecta/metabolism MH - Gene Expression Regulation, Plant PMC - PMC9737463 OTO - NOTNLM OT - Oryza sativa OT - defense system OT - jasmonic acid OT - pathogen OT - salicylic acid OT - transcriptomics COIS- The authors declare no conflict of interest. EDAT- 2022/12/12 06:00 MHDA- 2022/12/15 06:00 PMCR- 2022/12/04 CRDT- 2022/12/11 01:21 PHST- 2022/11/18 00:00 [received] PHST- 2022/11/29 00:00 [revised] PHST- 2022/11/29 00:00 [accepted] PHST- 2022/12/11 01:21 [entrez] PHST- 2022/12/12 06:00 [pubmed] PHST- 2022/12/15 06:00 [medline] PHST- 2022/12/04 00:00 [pmc-release] AID - ijms232315308 [pii] AID - ijms-23-15308 [pii] AID - 10.3390/ijms232315308 [doi] PST - epublish SO - Int J Mol Sci. 2022 Dec 4;23(23):15308. doi: 10.3390/ijms232315308.